Blastomyces dermatitidisseptinsCDC3, CDC10,andCDC12impact the morphology of yeast and hyphae, but are not required for the phase transition

Marty, Amber J.; Gauthier, Gregory M.

doi:10.3109/13693786.2012.699685

Cited by 5 publications

(3 citation statements)

References 42 publications

(84 reference statements)

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“…Thus, as had been seen for other thermally dimorphic fungus, T. cutaneum B3 also showed morphological switch that was related to the temperature of cultivation. But the relationship between dimorphic transition and temperature of cultivation in T. cutaneum was the reverse of that described by Penicillium marneffei [53] and Blastomyces dermatitidis [54].…”

Section: Dimorphic Transition In T Cutaneum Is Reversiblementioning

confidence: 76%

Influence of environmental and nutritional conditions on yeast–mycelial dimorphic transition in Trichosporon cutaneum

Zhu

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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Trichosporon cutaneum is able to metabolize high hydrophobic natural compounds such as fatty acids and also can be used as an effective biodegrader to remove a number of toxic aromatic compounds from the environment. However, its growth characteristics were poorly investigated and no yeast-mycelium dimorphism process has been established yet. In the present study, we provided first insights into the effect of nitrogen sources, carbon source, and amino acids together with pH and temperature on morphological switch of T. cutaneum B3. The results showed under close to neutral or weakly alkaline pH conditions, T. cutaneum B3 produced mostly yeast-like cells; while under acidic pH conditions, it produced mostly hyphal-like cells. Under buffered conditions, low nitrogen concentration (<0.2%) would facilitate T. cutaneum B3 to produce yeast-like cells, while the presence of relative high nitrogen concentration (>1%) would induce hyphal-like cells. Under non-buffered conditions, ammonium sulphate, diammonium phosphate, urea and Nacetylglucosamine may via alteration of environmental pH affect yeast-mycelium dimorphism transitions. Methionine, tryptophan and histidine invariably induce pseudohyphal or hyphal morphology. 25 and 28 C can promote yeast-like cells growth, while cultivated at 37 C can induce hyphal-like cells growth. Thus, the nitrogen source, alteration of environmental pH and temperature of cultivation played an important role in inducing yeast-mycelium dimorphism transition. Our study confirms the yeast-mycelium dimorphism process of T. cutaneum B3 and highlights that it seems to be a suitable yeast model for further molecular genetics investigation of dimorphism and applications in fermentation morphology engineering.

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Section: Dimorphic Transition In T Cutaneum Is Reversiblementioning

confidence: 76%

Influence of environmental and nutritional conditions on yeast–mycelial dimorphic transition in Trichosporon cutaneum

Zhu

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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“…To quantify the percentage of cells with yeast morphology, germ tube development, and hyphal growth, cells were stained with calcofluor white (Sigma Aldrich) [ 74 ]. Calcofluor white staining facilitated differentiation of germ tubes and hyphae by the absence or presence of septae, respectively.…”

Section: Methodsmentioning

confidence: 99%

Fungal Morphology, Iron Homeostasis, and Lipid Metabolism Regulated by a GATA Transcription Factor in Blastomyces dermatitidis

et al. 2015

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In response to temperature, Blastomyces dermatitidis converts between yeast and mold forms. Knowledge of the mechanism(s) underlying this response to temperature remains limited. In B. dermatitidis, we identified a GATA transcription factor, SREB, important for the transition to mold. Null mutants (SREBΔ) fail to fully complete the conversion to mold and cannot properly regulate siderophore biosynthesis. To capture the transcriptional response regulated by SREB early in the phase transition (0–48 hours), gene expression microarrays were used to compare SREB∆ to an isogenic wild type isolate. Analysis of the time course microarray data demonstrated SREB functioned as a transcriptional regulator at 37°C and 22°C. Bioinformatic and biochemical analyses indicated SREB was involved in diverse biological processes including iron homeostasis, biosynthesis of triacylglycerol and ergosterol, and lipid droplet formation. Integration of microarray data, bioinformatics, and chromatin immunoprecipitation identified a subset of genes directly bound and regulated by SREB in vivo in yeast (37°C) and during the phase transition to mold (22°C). This included genes involved with siderophore biosynthesis and uptake, iron homeostasis, and genes unrelated to iron assimilation. Functional analysis suggested that lipid droplets were actively metabolized during the phase transition and lipid metabolism may contribute to filamentous growth at 22°C. Chromatin immunoprecipitation, RNA interference, and overexpression analyses suggested that SREB was in a negative regulatory circuit with the bZIP transcription factor encoded by HAPX. Both SREB and HAPX affected morphogenesis at 22°C; however, large changes in transcript abundance by gene deletion for SREB or strong overexpression for HAPX were required to alter the phase transition.

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“…When initiated to grow in the hyphal state, C. albicans cdc10Δ, cdc11Δ and cdc12-6 temperature sensitive mutants develop curved hyphae and cell wall deposition is abnormal (Figure 2c)[34,44*]. Likewise, when septins are depleted from Blastomyces dermatitidis , hyphae are curved and thicker than control cells [47]. In A. nidulans , aspbΔ mutant cells display the emergence of bent germ tubes in contrast to wild-type conidia which generally grow via a single straight germ tube [36].…”

Section: What Is the Role Of Septins In Determining The Stability Andmentioning

confidence: 99%

Fungal pathogens are platforms for discovering novel and conserved septin properties

Bridges

Gladfelter

2014

Current Opinion in Microbiology

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Septins are filament-forming GTP-binding proteins that act as scaffolds in diverse cell functions including division, polarity and membrane remodeling. In a variety of fungal pathogens, it has been observed that septins are required for virulence because cells are unable to survive or are misshapen when septins are mutated. Cell morphology is interconnected with pathogenesis and thus septin mutants displaying aberrant cell morphologies are commonly deficient in host tissue invasion. The degree to which septins orchestrate versus maintain changes in fungal cell morphology during pathogenesis remains to be determined. Aside from the importance of septins in the process of pathogenesis, animal and plant fungal pathogens display complexity in septin form, dynamics, and function not seen in S. cerevisiae making these organisms important models for uncovering diversity in septin behavior. Additionally, host septins have recently been implicated in the process of C. albicans invasion, motivating the need to examine host septins in fungal pathogenesis. Understanding the role of septins in the host-pathogen interaction not only illuminates pathogenesis mechanisms but importantly also expands our understanding of septin biology in general.

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Blastomyces dermatitidisseptinsCDC3, CDC10,andCDC12impact the morphology of yeast and hyphae, but are not required for the phase transition

Cited by 5 publications

References 42 publications

Influence of environmental and nutritional conditions on yeast–mycelial dimorphic transition in Trichosporon cutaneum

Influence of environmental and nutritional conditions on yeast–mycelial dimorphic transition in Trichosporon cutaneum

Fungal Morphology, Iron Homeostasis, and Lipid Metabolism Regulated by a GATA Transcription Factor in Blastomyces dermatitidis

Fungal pathogens are platforms for discovering novel and conserved septin properties

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