<i>bla</i>
            <sub>VIM-2</sub>
            -Harboring Integrons Isolated in India, Russia, and the United States Arise from an Ancestral Class 1 Integron Predating the Formation of the 3′ Conserved Sequence

Toleman, Mark A.; Vinodh, Hemalatha; Sekar, Uma; Kamat, Vijaylakshmi; Walsh, Timothy R.

doi:10.1128/aac.01043-06

Cited by 49 publications

(45 citation statements)

References 16 publications

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“…This result is consistent with findings from Japan and Tunis (Hammami et al, 2010) in which the integron carrying aacA7 and bla VIM-2 at the 59 end did not possess the 39CS. In addition, similar results have been reported in P. aeruginosa isolates (strain RON-1) isolated in Paris (Poirel et al, 2006), Portugal (Quinteira & Peixe, 2006) and India (Toleman et al, 2007). However, the downstream regions of the bla VIM-2 gene of these isolates were found to be different when compared with the P. aeruginosa isolate used in our study.…”

Section: Discussionsupporting

confidence: 72%

Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia

Khosravi

Tay

Vadivelu

2011

Journal of Medical Microbiology

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In this study, 90 non-replicate imipenem-resistant Pseudomonas aeruginosa (IRPA) Malaysian isolates collected between October 2005 and March 2008 were subjected to a screening test for detection of the integron and the gene cassette. Class 1 integrons were detected in 54 IRPA clinical isolates, whilst three isolates contained class 2 integrons. Analysis of the gene cassettes associated with the class 1 integrons showed the detection of accC1 in isolates carrying bla and aacA7 in isolates carrying bla VIM-2 . aadA6 was detected in two isolates carrying bla . Using random amplification of polymorphic DNA analysis, 14 PCR fingerprint patterns were generated from the 32 isolates carrying metallo-b-lactamase (MBL) genes (35.5 %), whilst 20 patterns were generated from the 58 non-MBL gene isolates (64.4 %). Based on the differences in the fingerprinting patterns, two clusters (A and B) were identified among the MBL-producing isolates. Cluster A comprised 18 isolates (56 %) carrying the bla VIM gene, whereas cluster B comprised 14 (44 %) isolates carrying the bla IMP gene. The non-MBL isolates were divided into clusters C and D. Cluster C comprised 22 non-MBL isolates harbouring class 1 integrons, whilst cluster D consisted of three isolates carrying class 2 integrons. These findings suggest that the class 1 integron is widespread among P. aeruginosa isolated in Malaysia and that characterization of cassette arrays of integrons will be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes. INTRODUCTIONThe recent emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa has become a serious problem in healthcare settings worldwide, including in Asian countries (Leung et al., 2008;Raja & Singh, 2007;Sekiguchi et al., 2007). The rapid emergence of antibiotic resistance among clinical isolates of bacteria is due to dissemination of antibiotic resistance genes by horizontal transfer (Leverstein-van Hall et al., 2003). Carbapenems, including imipenem and meropenem, are the most potent antimicrobial agents for the treatment of P. aeruginosa infections (Lee et al., 2011). However, carbapenem resistance due to metallo-b-lactamase (MBL) production of P. aeruginosa has been reported (Varaiya et al., 2008). Several families of MBLs have been documented in P. aeruginosa, including IMP, VIM, SPM, GIM, SIM, KHM, AIM and NDM (Toleman & Walsh, 2008). The genes of both IMP-and VIM-type MBLs (bla IMP and bla VIM ) in clinical isolates of P. aeruginosa are often encoded on mobile gene cassettes inserted into class 1 integrons (Pasteran et al., 2005;Peleg et al., 2004;Yong et al., 2009;Zhao et al., 2009). Integrons have been identified as a primary source of resistance genes and are suspected to serve as reservoirs of antimicrobial resistance genes within microbial populations (Xu et al., 2009). They are able to capture one or more gene cassettes from the environment and incorporate them using site-specific recombination. To date, there are more than nine cla...

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Section: Discussionsupporting

confidence: 72%

Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia

Khosravi

Tay

Vadivelu

2011

Journal of Medical Microbiology

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“…A class 1 integron carrying aacA7 and bla VIM-2 was described in Romania (30), but the downstream region of the bla VIM-2 gene was different when compared with the sequences from our study. In particular, the former integron had three gene cassettes in the variable region, aacA7, bla VIM-2 , dhfrB, similar to integrons found in Russia, United States and India (31). Almost all class 1 integrons described in our study had only two cassettes (aacA7 and bla VIM-2 ).…”

Section: Discussionmentioning

confidence: 76%

Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania

Mereuţă¹,

Badescu²,

Dorneanu³

et al. 2013

Romanian Review of Laboratory Medicine

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“…Another study from India also reported bla VIM-2 from P. aeruginosa [22]. In a nation-wide survey conducted to characterise 301 MBL producing Pseudomonas species in 10 medical centres from India, the MBL genes were detected in 18.9% of the isolates and 5 VIM variants were reported with VIM-2 being the most common.…”

Section: Discussionmentioning

confidence: 99%

blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India

Amudhan

Sekar

Arunagiri

et al. 2012

J Infect Dev Ctries

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Introduction: The emergence and rapid spread of bla IMP and bla VIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. Methodology: A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61), Acinetobacter baumannii (116), Acinetobacter lwoffii (1) and Pseudomonas stutzeri (1) isolated from patients hospitalized for 48 hours or more were included in the study. The minimum inhibitory concentrations (MIC) to imipenem and meropenem were determined and interpreted according to Clinical Laboratory Standards Institute guidelines. The Modified Hodge Test (MHT) and inhibitor potentiated disk diffusion tests with ethylenediaminetetraacetic acid (EDTA) were used for screening of carbapenamases and MBL production respectively. Polymerase chain reaction (PCR) was performed for the detection of MBL (bla VIM and bla IMP ) genes. Gene sequencing was performed for representative isolates. Results: MHT was positive in 94.4% (n = 169). MBL screening with EDTA was positive in 80.4% (n = 144). MBL genes bla VIM and bla IMP were detected in 92 (51.4%) isolates. Bla VIM alone was detected in 89 isolates while two isolates had bla IMP alone. One isolate had both bla

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bla _VIM-2 -Harboring Integrons Isolated in India, Russia, and the United States Arise from an Ancestral Class 1 Integron Predating the Formation of the 3′ Conserved Sequence

Cited by 49 publications

References 16 publications

Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia

Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia

Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania

blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India

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bla VIM-2 -Harboring Integrons Isolated in India, Russia, and the United States Arise from an Ancestral Class 1 Integron Predating the Formation of the 3′ Conserved Sequence

Cited by 49 publications

References 16 publications

Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia

Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia

Spread of VIM-2 metallo-beta-lactamase in Pseudomonas aeruginosa and Acinetobacter baumannii clinical isolates from Iaşi, Romania

blaIMP and blaVIM mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India

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Product

Resources

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bla _VIM-2 -Harboring Integrons Isolated in India, Russia, and the United States Arise from an Ancestral Class 1 Integron Predating the Formation of the 3′ Conserved Sequence