<i>Bifidobacterium animalis</i>
            subsp.
            <i>lactis</i>
            fermented milk product reduces inflammation by altering a niche for colitogenic microbes

Veiga, Patrick; Gallini, Carey Ann; Beal, Chloé; Michaud, Monia; Delaney, Mary L.; DuBois, Andrea M.; Khlebnikov, Artem; Vlieg, Johan E. T. van Hylckama; Punit, Shivesh; Glickman, Jonathan N.; Onderdonk, Andrew B.; Glimcher, Laurie H.; Garrett, Wendy S.

doi:10.1073/pnas.1011737107

Cited by 197 publications

(180 citation statements)

References 43 publications

Supporting

Mentioning

164

Contrasting

Unclassified

Order By: Relevance

“…Our observation is in accord with pre- vious reports suggesting that monocarboxylic acid transporters provide a link between bacteria and the host (47)(48)(49). In human healthy adults, no or low (0 -2 mM) lactate is detected in fecal samples (50,51) because the luminal lactate is absorbed by host and is also used as an energy source by other commensal bacteria (52)(53)(54). However, increased fecal lactate has been associated with intestinal malabsorption and colitis (55).…”

Section: Discussionsupporting

confidence: 74%

Impact of the Metabolic Activity of Streptococcus thermophilus on the Colon Epithelium of Gnotobiotic Rats

Rul

Ben-Yahia

Chegdani

et al. 2011

Journal of Biological Chemistry

View full text Add to dashboard Cite

The thermophilic lactic acid bacterium Streptococcus thermophilus is widely and traditionally used in the dairy industry. Despite the vast level of consumption of S. thermophilus through yogurt or probiotic functional food, very few data are available about its physiology in the gastrointestinal tract (GIT). The objective of the present work was to explore both the metabolic activity and host response of S. thermophilus in vivo. Our study profiles the protein expression of S. thermophilus after its adaptation to the GIT of gnotobiotic rats and describes the impact of S. thermophilus colonization on the colonic epithelium. S. thermophilus colonized progressively the GIT of germ-free rats to reach a stable population in 30 days (10 8 cfu/g of feces). This progressive colonization suggested that S. thermophilus undergoes an adaptation process within GIT. Indeed, we showed that the main response of S. thermophilus in the rat's GIT was the massive induction of the glycolysis pathway, leading to formation of lactate in the cecum. At the level of the colonic epithelium, the abundance of monocarboxylic acid transporter mRNAs (SLC16A1 and SLC5A8) and a protein involved in the cell cycle arrest (p27 kip1 ) increased in the presence of S. thermophilus compared with germ-free rats. Based on different mono-associated rats harboring two different strains of S. thermophilus (LMD-9 or LMG18311) or weak lactate-producing commensal bacteria (Bacteroides thetaiotaomicron and Ruminococcus gnavus), we propose that lactate could be a signal produced by S. thermophilus and modulating the colon epithelium.Streptoccocus thermophilus belongs to the group of the thermophilic lactic acid bacteria and is traditionally and widely used as a starter in manufacturing dairy products (Emmental, Gruyere, Parmigiano, mozarella, yogurt, etc.). Yogurt, which results from the fermentation of milk by S. thermophilus and Lactobacillus delbrueckii sp. bulgaricus (L. bulgaricus), fulfills the current specifications required to be recognized as a probiotic product (1). The health beneficial effect of yogurt consumption is linked to the metabolic properties of S. thermophilus and L. bulgaricus. As such, it improves lactose digestion in the gastrointestinal tract (GIT) 3 through their lactose-hydrolyzing activity present in yogurt and in the GIT, thus reducing symptoms of lactose intolerance (2, 3).Yogurt cultures have been shown to induce other health benefits, such as reduction of diarrhea or allergic disorders as well as modulation of the immune system (1, 4). S. thermophilus is also present at high concentration in VSL#3, a probiotic mixture of eight different bacterial strains that possesses beneficial effects in several intestinal conditions (5, 6).Recent data indicate that strains related to S. thermophilus LMD-9 are among the 57 bacteria species found in the intestinal microbiota of 90% of 124 European individuals (7). In comparison with the overall human intestinal microbiota, S. thermophilus is a numerically nondominant species with variable levels...

show abstract

Section: Discussionsupporting

confidence: 74%

Impact of the Metabolic Activity of Streptococcus thermophilus on the Colon Epithelium of Gnotobiotic Rats

Rul

Ben-Yahia

Chegdani

et al. 2011

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The addition of the probiotic caused a metabolic shift in the microbiota which resulted in a non-permissive environment for opportunistic pathogens. 59 A more recent report by Eloe-Fadrosh et al 43 examined the dynamics of the gut transcriptome following probiotic consumption. They identified an increase in flagellar motility, chemotaxis, and adhesion genes from resident commensal bacteria which were positively regulated by the addition of the probiotic to the host diet.…”

Section: Discussionmentioning

confidence: 99%

Impact of probiotic supplements on microbiome diversity following antibiotic treatment of mice

2016

View full text Add to dashboard Cite

Shifts in microbial populations of the intestinal tract have been associated with a multitude of nutritional, autoimmune, and infectious diseases. The limited diversity following antibiotic treatments creates a window for opportunistic pathogens, diarrhea, and inflammation as the microbiome repopulates. Depending on the antibiotics used, microbial diversity can take weeks to months to recover. To alleviate this loss of diversity in the intestinal microbiota, supplementation with probiotics has become increasingly popular. However, our understanding of the purported health benefits of these probiotic bacteria and their ability to shape the microbiome is significantly lacking. This study examined the impact of probiotics concurrent with antibiotic treatment or during the recovery phase following antibiotic treatment of mice. We found that probiotics did not appear to colonize the intestine themselves or shift the overall diversity of the intestinal microbiota. However, the probiotic supplementation did significantly change the types of bacteria which were present. In particular, during the recovery phase the probiotic caused a suppression of Enterobacteriaceae outgrowth (Shigella and Escherichia) while promoting a blooming of Firmicutes, particularly from the Anaerotruncus genus. These results indicate that probiotics have a significant capacity to remodel the microbiome of an individual recovering from antibiotic therapy.

show abstract

“…Histology. Sections were examined and degree of colitis was scored as described previously (50) and in SI Materials and Methods.…”

Section: Methodsmentioning

confidence: 99%

QseC inhibition as an antivirulence approach for colitis-associated bacteria

Rooks

Veiga

Reeves

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Hosts and their microbes have established a sophisticated communication system over many millennia. Within mammalian hosts, this dynamic cross-talk is essential for maintaining intestinal homeostasis. In a genetically susceptible host, dysbiosis of the gut microbiome and dysregulated immune responses are central to the development of inflammatory bowel disease (IBD). Previous surveys of stool from the T-bet −/− Rag2 −/− IBD mouse model revealed microbial features that discriminate between health and disease states. Enterobacteriaceae expansion and increased gene abundances for benzoate degradation, two-component systems, and bacterial motility proteins pointed to the potential involvement of a catecholamine-mediated bacterial signaling axis in colitis pathogenesis. Enterobacteriaceae sense and respond to microbiota-generated signals and host-derived catecholamines through the two-component quorum-sensing Escherichia coli regulators B and C (QseBC) system. On signal detection, QseC activates a cascade to induce virulence gene expression. Although a single pathogen has not been identified as a causative agent in IBD, adherent-invasive Escherichia coli (AIEC) have been implicated. Flagellar expression is necessary for the IBD-associated AIEC strain LF82 to establish colonization. Thus, we hypothesized that qseC inactivation could reduce LF82's virulence, and found that an absence of qseC leads to down-regulated flagellar expression and motility in vitro and reduced colonization in vivo. We extend these findings on the potential of QseC-based IBD therapeutics to three preclinical IBD models, wherein we observe that QseC blockade can effectively modulate colitogenic microbiotas to reduce intestinal inflammation. Collectively, our data support a role for QseC-mediated bacterial signaling in IBD pathogenesis and indicate that QseC inhibition may be a useful microbiota-targeted approach for disease management.colitis | Escherichia coli | QseC | antivirulence | gut microbiome

show abstract

Bifidobacterium animalis subsp. lactis fermented milk product reduces inflammation by altering a niche for colitogenic microbes

Cited by 197 publications

References 43 publications

Impact of the Metabolic Activity of Streptococcus thermophilus on the Colon Epithelium of Gnotobiotic Rats

Impact of the Metabolic Activity of Streptococcus thermophilus on the Colon Epithelium of Gnotobiotic Rats

Impact of probiotic supplements on microbiome diversity following antibiotic treatment of mice

QseC inhibition as an antivirulence approach for colitis-associated bacteria

Contact Info

Product

Resources

About