Bacillus subtilis strain BS06 protects soybean roots from Fusarium oxysporum infection

Abstract

Soybean, as a major oil crop, is one of the most widely planted crops in the world. Fusarium oxysporum causes soybean root rot, leading to great economic losses to soybean planting every year globally. Chemical fungicide for controlling soybean F. oxysporum diseases may cause environmental problems and have human health risks. Biological control methods avoid these shortcomings, however, few studies have focused on biocontrol of soybean diseases caused by F. oxysporum. Aiming at this problem, we obtained biocontrol bacteria against soybean F. oxysporum by plate confrontation method. The type of the strain with the highest biocontrol activity was identified by molecular biological methods, and then verified it biocontrol effects through greenhouse experiments. One of our isolated strain named BS06 strain had the highest activity, which was identified as Bacillus subtilis. Our study showed that BS06 strain could effectively control soybean F. oxysporum disease and significantly reduce F. oxysporum to infect soybean roots. Compared with control and carbendazim treatments, BS06 treatment had higher root biomass, plant height, leaf chlorophyll content, stem base diameter and control efficiency. Our results indicated that BS06 could effectively protect soybean root, that might BS06 strain produce substances to inhibit F. oxysporum, which was potentially useful for soybean planting.

Metabolomic and Transcriptome Analysis of the Inhibitory Effects of Bacillus subtilis Strain Z-14 against Fusarium oxysporum Causing Vascular Wilt Diseases in Cucumber

“…7,8 B. subtilis BS06 isolated from soybean rhizosphere can be used to effectively control F. oxysporum (FO) and significantly reduce FO infection of soybean roots. 9 In recent years, the biological control of FO was accomplished with new strains of Bacillus and enhanced control mechanism. Bacillus bm02 isolated from tomato rhizosphere significantly reduced the severity of tomato Fusarium wilt, which released many bioactive compounds such as phenylacetic acid (PAA) and methylphenylacetic acid (MPA) with inhibitory effects on plant pathogens such as FO.…”

Multi-Omics Techniques for Analysis Antifungal Mechanisms of Lipopeptides Produced by Bacillus velezensis GS-1 against Magnaporthe oryzae In Vitro

“…In recent years, Bacillus spp. have provided a useful strategy for management of F. graminearum , F. oxysporum , and Xanthomonas axonopodis [ 8 , 12 , 21 , 24 , 34 ]. In addition, Bacillus spp.…”

“…have been used to control rice blast in the field [ 2 , 3 , 4 , 5 ]. Among microbial candidates used for biocontrol, Bacillus species are most attractive because they can produce spores, and a series of bioactive compounds allow them to survive in adverse environmental conditions [ 6 , 7 , 8 ]. Moreover, Bacillus species have many functions, which include promotion of plant growth and stress resistance, improvement in soil fertility, and remediation of contaminated soil [ 9 , 10 , 11 , 12 , 13 ].…”

“…The endophytic Bacillus subtilis and Bacillus megaterium isolated from wheat inhibited vegetative growth and conidia germination of Fusarium graminearum significantly [ 14 ]. B. subtilis BS06 could effectively protect soybean root caused by Fusarium oxysporum [ 8 ]. The antagonistic strain B. velezensis SDTB038 achieved good control of potato late blight in greenhouses and fields and promoted potato plant growth [ 12 ].…”

A non-destructive testing method for early detection of ginseng root diseases using machine learning technologies based on leaf hyperspectral reflectance

“…Root diseases were often accompanied with a decrease in chlorophyll content of leaves ( Fujimoto et al., 2021 ; Jia et al., 2021 ), which indicated the reduction of plant photosynthesis and external stresses ( Monteoliva et al., 2021 ). In addition, the contents of antioxidant enzymes in leaves are also affected by root diseases ( Harrach et al., 2013 ).…”