Bacillus subtilis
 CK3 used as an aquatic additive probiotics enhanced the immune response of crayfish
 Procambarus clarkii
 against newly identified
 Aeromonas veronii
 pathogen

Yang, Hui; Zhao, Meng; Ji, Tongwei; Zhang, Yingying; Wei, Wenzhi; Liu, Qiu-Ning

doi:10.1111/are.15571

Cited by 6 publications

(7 citation statements)

References 46 publications

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“…Probiotics can improve fish immunity, which may be due to the fact that the added probiotics reduce the ammonia nitrogen content in the water body and create a relatively good living environment for fish (Banerjee & Ray, 2017; Kuebutornye et al ., 2019; Soltani et al ., 2019). Furthermore, probiotics can also produce antibacterial active substances, which can resist the colonization and invasion of pathogenic bacteria and improve the immunity of fish (Dawood et al ., 2019, 2020; Gabriel et al ., 2012; Maas et al ., 2021; Naiel et al ., 2022; Poolsawat et al ., 2020; Rohani et al ., 2022; Van Doan et al ., 2020; Yang et al ., 2021).…”

Section: Discussionmentioning

confidence: 99%

Effect of Bacillus amyloliquefaciensLSG2‐8 on the intestinal barrier function of Amur minnow (Rhynchocypris lagowskii)

Zhu

Wang

et al. 2023

Journal of Fish Biology

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Probiotics are widely used in aquaculture. This article aims to study the effect of Bacillus amyloliquefaciens LSG2‐8 on the intestinal barrier function of Rhynchocypris lagowskii. B. amyloliquefaciens LSG2‐8 were added to R. lagowskii basal diets (CK) as additives at four concentrations: 1.0 × 106 (D‐6), 1.0 × 107 (D‐7), 1.0 × 108 (D‐8) and 1.0 × 109 (D‐9) CFU g−1 by dry weight of basal diet. After a 56‐day feeding experiment, the activities of intestinal digestive enzymes and immunity‐related enzymes of R. lagowskii on group D‐6, D‐7, D‐8 and D‐9 diet were significantly higher than the control (P < 0.05). In molecular experiments, the authors found that the levels of TGF‐β mRNA, IL‐10 mRNA, ZO‐1 mRNA and claudin‐3 mRNA in group D‐8 R. lagowskii were significantly higher (P < 0.05) than those of the control and other groups. Furthermore, the levels of IL‐1β and IL‐8 mRNA of R. lagowskii on group D‐6, D‐7, D‐8 and D‐9 diet were significantly lower than those of the control (P < 0.05). In addition, the authors found that B. amyloliquefaciens LSG2‐8 can regulate the intestinal flora balance and improve the intestinal structure of R. lagowskii. In conclusion, B. amyloliquefaciens LSG2‐8 can improve the intestinal barrier function of R. lagowskii and can be used as a feed additive in aquaculture.

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Section: Discussionmentioning

confidence: 99%

Effect of Bacillus amyloliquefaciensLSG2‐8 on the intestinal barrier function of Amur minnow (Rhynchocypris lagowskii)

Zhu

Wang

et al. 2023

Journal of Fish Biology

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“…The prevalence and abundance of probiotics in GIT of fish fed with Diet B and C as compared to their absence in the gastrointestinal tract of the fishes fed with Diet A is an indication that insect protein (especially grasshopper protein) is of good and quality feed and can be used to supplement fish meal in feed diet of fishes. The probiotic bacteria presence in the supplementary feed has the capacity to increase the health status as well as growth performance of fish [18,19,37].…”

Section: Phylogenetic Tree Of Some Bacteria Isolatesmentioning

confidence: 99%

“…Probiotics have been employed as a disease control method in aquaculture activities particularly those caused by infection of bacteria [16][17][18][19]. Lactic acid bacteria which originate in the GIT of fish are form of probiotic bacteria that are of benefit to fish health through greater nutrient utilization and antimicrobial capabilities [17].…”

Section: Introductionmentioning

confidence: 99%

“…Lactic acid bacteria which originate in the GIT of fish are form of probiotic bacteria that are of benefit to fish health through greater nutrient utilization and antimicrobial capabilities [17]. Probiotics boost the immune response to pathogenic illnesses in fish [18][19][20]. Various studies have demonstrated that specific probiotic groups have antibacterial action in vitro on rainbow trout [21], common carp [22], Tilapia [23] and other species of freshwater fishes [24,25].…”

Section: Introductionmentioning

confidence: 99%

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Probiotic Bacteria in Gastro-Intestinal Tract of Hybrid Catfish Fed Insect Protein Meal

Aliyu‐Paiko

Iloba

Adediran

et al. 2023

AJFAR

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The evaluations of insects’ protein as viable alternative protein source in fish diets are gaining more attention revealing that the protein has more comparative advantage than other protein sources. Therefore, this study was conducted to isolate and identify (using phylotypic and genotypic methods) bacteria isolates in the gastrointestinal tract (GIT) of African hybrid catfish fed supplemented grasshopper meal. Four feeding diets were adopted in this study; Diet A (conventional feed), Diet B (100% Grasshopper meal), Diet C (50% fishmeal and 50% Grasshopper meal) and Diet D (100% Fish meal). At the end of the 12- week experimental period, the fish were sacrificed for their GIT contents to determine bacteria diversity. In the catfish fed diet A, the bacteria isolates identified were Streptococcus pyogenes, Enterobacter aerogene, Staphylococcus aureus, Shigella flexneri, Escherichia coli, Leuconostoc latis and Klebsella pneumonia. In catfish fed diet B, Bacillus subtilis, Lactococcus bulgaricus, Lysinibacillus macroides, Lysinibacillus capsici, Stenotrophomonas pavanii and Lysinibacillus sp were isolated. Similarly, the fish fed diet C recorded the presence of Bacillus subtilis, Lactococcus bulgaricus, Lysinibacillus sp., Staphylococcus aureus, Lysinibacillus macroides, Stenotrophomonas pavanii and Enterobacter aerogene. The bacteria isolated in the GIT of fish fed diet D were Bacillus cereus, Staphylococcus aureus, Pedicoccus acidilactis, Escherichia coli, Salmonella typhi, Leuconostoc latis and Shigella flexneri. Probiotic bacteria were more in fish fed with diets B and C, an indication that the different ratio of supplemented grasshopper meal is good for the fish, as they have the potentials to boost the fish immune system and improve the microbial balance of its gastro-intestinal tract.

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“…It causes not only human diseases such as meningitis, diarrhea, and trauma infection ( Cui et al, 2007 ; Prediger et al, 2020 ; Zhang et al, 2020 ), but also aquaculture diseases such as rot skin disease, septicemia, and rotten tail disease ( Dong et al, 2017 ; Lazado and Zilberg, 2018 ; Liu Z. G. et al, 2018 ; Ran et al, 2018 ). In addition, A. veronii is resistant to many antibiotics together with penicillin, ampicillin, oxacillin and piperacillin ( Yang et al, 2022 ). So it’s intractable to prevent and treat, and it is urgent to study the regulation mechanism of its virulence.…”

Section: Introductionmentioning

confidence: 99%

Peptide Aptamer PA3 Attenuates the Viability of Aeromonas veronii by Hindering of Small Protein B-Outer Membrane Protein A Signal Pathway

Liu

Chang

et al. 2022

Front. Microbiol.

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The small protein B (SmpB), previously acting as a ribosome rescue factor for translation quality control, is required for cell viability in bacteria. Here, our study reveals that SmpB possesses new function which regulates the expression of outer membrane protein A (ompA) gene as a transcription factor in Aeromonas veronii. The deletion of SmpB caused the lower transcription expression of ompA by Quantitative Real-Time PCR (qPCR). Electrophoretic mobility shift assay (EMSA) and DNase I Footprinting verified that the SmpB bound at the regions of −46 to −28 bp, −18 to +4 bp, +21 to +31 bp, and +48 to +59 bp of the predicted ompA promoter (PompA). The key sites C52AT was further identified to interact with SmpB when PompA was fused with enhanced green fluorescent protein (EGFP) and co-transformed with SmpB expression vector for the fluorescence detection, and the result was further confirmed in microscale thermophoresis (MST) assays. Besides, the amino acid sites G11S, F26I, and K152 in SmpB were the key sites for binding to PompA. In order to further develop peptide antimicrobial agents, the peptide aptamer PA3 was screened from the peptide aptamer (PA) library by bacterial two-hybrid method. The drug sensitivity test showed that PA3 effectively inhibited the growth of A. veronii. In summary, these results demonstrated that OmpA was a good drug target for A. veronii, which was regulated by the SmpB protein and the selected peptide aptamer PA3 interacted with OmpA protein to disable SmpB-OmpA signal pathway and inhibited A. veronii, suggesting that it could be used as an antimicrobial agent for the prevention and treatment of pathogens.

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Bacillus subtilis CK3 used as an aquatic additive probiotics enhanced the immune response of crayfish Procambarus clarkii against newly identified Aeromonas veronii pathogen

Abstract: The Aeromonads, including Aeromonas caviae, Aeromonas hydrophila, Aeromonas veronii and Aeromonas sobria, are widely distributed in aquatic ecosystems (such as lakes, rivers and coastal water)

Cited by 6 publications

References 46 publications

Effect of Bacillus amyloliquefaciensLSG2‐8 on the intestinal barrier function of Amur minnow (Rhynchocypris lagowskii)

Effect of Bacillus amyloliquefaciensLSG2‐8 on the intestinal barrier function of Amur minnow (Rhynchocypris lagowskii)

Probiotic Bacteria in Gastro-Intestinal Tract of Hybrid Catfish Fed Insect Protein Meal

Peptide Aptamer PA3 Attenuates the Viability of Aeromonas veronii by Hindering of Small Protein B-Outer Membrane Protein A Signal Pathway

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