Atopobium and Fusobacterium as novel candidates for sarcoidosis-associated microbiota

Abstract

Sarcoidosis is a granulomatous disease that mainly affects the lung. A role of microbial factors in disease pathogenesis is assumed, but has not been investigated systematically in a large cohort.This cross-sectional study compared the lung microbiota of 71 patients with sarcoidosis, 15 patients with idiopathic pulmonary fibrosis (non-infectious controls) and 10 healthy controls (HCs). Next-generation sequencing of 16S DNA was used on bronchoalveolar lavage samples to characterise the microbial composition, which was analysed for diversity and indicator species. Host genotypes for 13 known sarcoidosis risk variants were determined and correlated with microbial parameters.The microbial composition differed significantly between sarcoidosis and HC samples (redundancy analysis ANOVA, p=0.025) and between radiographic Scadding types. spp. was detected in 68% of sarcoidosis samples, but not in HC samples. spp. was significantly more abundant in sarcoidosis samples compared with those from HCs. Mycobacteria were found in two of 71 sarcoidosis samples. Host-genotype analysis revealed an association of the rs2076530 () risk allele with a decrease in bacterial burden (p=0.002).Our results indicate Scadding type-dependent microbiota in sarcoidosis BAL samples. spp. and spp. were identified as sarcoidosis-associated bacteria, which may enable new insights into the pathogenesis and treatment of the disease.

Malnutrition Accelerates Colorectal Cancer Progression through Macrophage Activated by B Cells Immune via Gut Microbiota

“…We next identi ed Atopobium, Selenomonas and Faecalibacterium may predict the different malnutrition status in CRC patients using ROC analysis. It brought to our attention that Atopobium and Fusobacterium were identi ed as sarcoidosis-associated bacteria, which may enable new insights into the pathogenesis and treatment of CRC [12]. In CRC, the abundance of Atopobium parvulum and Actinomyces odontolyticus, were signi cantly increased only in multiple polypoid adenomas and/or intramucosal carcinomas, indicating those two bacteria may be considered as the microbiome therapy target in CRC [13].…”

Are infectious diseases risk factors for sarcoidosis or a result of reverse causation? Findings from a population-based nested case–control study

“…In fact, infectious agents gained the most research attention throughout the years largely due to the clinical and histological similarities between sarcoidosis and tuberculosis. Except mycobacteria [4,5], the role of other bacteria such as propionibacteria [6][7][8][9], as well as viruses [10], fungi [11,12] and the lung microbiome overall [13,14] has been investigated in numerous molecular studies. The causal role of these infectious agents is still obscure as results are conflicting.…”

To progress understanding of disease triggers and modifiers in sarcoidosis, stratification is the key

“…In this issue of the European Respiratory Journal, ZIMMERMANN et al [6] present the largest study so far and describe the microbial composition in 71 patients with sarcoidosis, 10 healthy controls and 15 IPF patients. Mycobacterial DNA was only present in two sarcoidosis patients.…”

“…Because the sample size in the ZIMMERMANN et al [6] study was large, the authors stratified the patients by lung phenotype according to radiographic Scadding stages. In comparison with controls, ZIMMERMANN et al [6] found that the increase in Atopobium sp. was associated with stage I (n=17) and II (n=39) and the increase in Fusobacterium sp.…”

“…BTNL2 is a disease risk locus for sarcoidosis [20]. In the study by ZIMMERMANN et al [6], stratification of patients by genotype of BTNL2 (rs2076530) showed that carriership of the risk allele A was significantly associated with a decrease in bacterial burden. BTNL2 is part of the HLA region and has immunomodulating properties.…”