<i>Arcobacter Cryaerophilus</i> and <i>Arcobacter Butzleri</i> Isolated from Preputial Fluid of Boars and Fattening Pigs in Brazil

Oliveria, Sergio J. de; Wesley, Irene V.; Baetz, A L; Harmon, Karen M.; Kader, Ivonyr Irene Tróglio Abdel; Uzeda, Milton de

doi:10.1177/104063879901100513

Cited by 22 publications

(12 citation statements)

References 16 publications

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“…The results demonstrated the invasive and virulence capacity of the A. butzleri strains, which was suggested to be hostdependent with respect to species and breed (20). Experimental infection in udder also produced clinical mastitis with fever and a reduced milk yield (21). In another study, venereal transmission was suggested as A. butzleri and A. cryaerophilus were isolated from the preputial fluid of boars and fattening pigs (22).…”

Section: Introductionmentioning

confidence: 87%

Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri

Adesiji¹,

Emikpe²,

Opalekunde³

2011

AML

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Background.Arcobacter is an emerging zoonotic enteropathogen associated with spontaneous abortion, diarrhea and mastitis in domestic animals.Materials and methods. Thirty male pathogen-free albino rats were infected with a single challenge of Arcobacter butzleri (10 8 cfu/ml) isolated from the stool of healthy pigs with the aim of investigating the effects of A. butzleri on rat testicular histology and spermatogenesis.Results. In previously healthy male albino rats, A. butzleri caused testicular degeneration associated with reduced sperm count and motility.Conclusions. The result of this study suggests that A. butzleri produces testicular degeneration and the associated disruption of spermatogenesis in albino rats; hence, its infertility potential in livestock industry and its economic importance should be further investigated. Key words:Arcobacter butzleri, testicular degeneration, infertility,pathogenicity, sperm count, albino rats INTRODUCTIONArcobacter is an emerging enteropathogen with an increasing evidence of the zoonotic potential associated with prolonged diarrhoea and occasional systemic infections such as bactereamia and peritonitis in humans (1). An evidencebased semi-quantitative method for prioritization of foodborne zoonoses ranked A. butzleri as a microbe of "significant importance" (2). Arcobacter was first isolated from aborted bovine and porcine fetuses (3), especially A. butzleri and A. skirrowii which were isolated from aborted fetuses and placenta of bovine, porcine and ovine origin (4). Although no remarkable pathological changes were identified, the clinical signs associated with Arcobacter infection include infertility, chronic discharge during oestrus, stillbirth and late-term abortions (5).The pathogenicity and virulence mechanisms of Arcobacter species are still poorly understood, despite its increasing incidence and isolation from clinical and food products (6). The virulence of A. cryaerophilus was also first described when it was observed that the strains tested induced the accumulation of fluid and electrolytes in the rat ileal loop assay and showed an in vitro invasion of Hep-2 128Yemisi Olukemi Adesiji, Benjamin Obukowho Emikpe, Ahmed Bayo Opalekunde cells (7). Several studies were reported on their adhesion capacity (8-10), invasiveness (9, 11), and cytotoxicity (12)(13)(14) in several cell lines. Collectively, in these studies, the strains tested showed adhesion, invasion, and cytotoxicity, toxicity and adherence being the most commonly observed effects. The differences observed among different studies may be due to the origin of the strains (environmental versus clinical) as well as to different cell lines used in the studies, A. butzleri being the most invasive species in experimental animal infections (15). Other animal models and in vivo experiments have been reviewed in another recent publication (16). The presence of adhesion molecules in A. butzleri have been proven by the capacity of the strains tested to agglutinate human, rabbit, and sheep erythrocytes, and hemagglut...

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Section: Introductionmentioning

confidence: 87%

Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri

Adesiji¹,

Emikpe²,

Opalekunde³

2011

AML

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“…A. cryaerophilus have been isolated from aborted pig fetuses from some countries, including Brazil (SCHROEDER-TUCKER et al, 1996;OLIVEIRA et al, 1997;1999). A. butzleri have been detected from enteritis in humans (LERNER et al, 1994;LAWERS et al, 1996;LAU et al, 2002;FERNANDEZ et al, 2004;VANDENBERG et al, 2004) and also the microorganisms were isolated from poultry and pig carcasses (RIDSDALE et al, 1998;OLIVEIRA et al, 2001;.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Arcobacter spp from the milk of dairy cows in Brazil

et al. 2007

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“…However, they have also been implicated in reproduction disorders as late-term abortions and a higher rate of stillbirths and have been isolated from internal organs of aborted pig fetuses and placental, uterine, and oviductal tissues (5,10,36). They are present in vaginal swabs of normal producing sows and were isolated from preputial fluid of boars on farms with reproductive problems, though they have not been detected in the semen (6,24). Nevertheless, insemination with experimentally infected semen seemed to induce a decrease in conception rates in sows.…”

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Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms

Smet

Zutter

Debruyne

et al. 2011

Appl Environ Microbiol

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Healthy pigs are an important reservoir for the emerging human pathogen Arcobacter which can result in contamination of porcine carcasses and pork and the spread of arcobacters into the environment. Up to now, the excretion of arcobacters by pigs has been studied, but information about the transmission routes in fattening pigs is lacking. The present study aimed to elucidate the Arcobacter population dynamics in pigs during the fattening period on four farrow-to-finish farms. On each farm, 30 clinically healthy, 12-week-old piglets were selected. Fecal samples were collected on 10 sampling occasions until a slaughter age of 30 weeks was reached. Arcobacter spp. were isolated by a selective method and identified by multiplex PCR. The genetic diversity was examined by amplified fragment length polymorphism and enterobacterial repetitive intergenic consensus PCR. The Arcobacter presence in the fecal samples on the four farms ranged from 11.3 to 50.0%, with excretion levels of up to 10 4 CFU/g feces. The ratio in which Arcobacter species were isolated varied between the farms and over time. Characterization revealed a high degree of genotypic diversity among the isolates. Arcobacter strains persisted and spread within the finishing unit during the fattening period. The occurrence of both unique and shared genotypes in pigs in adjacent and nonadjacent pens demonstrates that transmission routes other than fecal-oral transmission occur.

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Arcobacter Cryaerophilus and Arcobacter Butzleri Isolated from Preputial Fluid of Boars and Fattening Pigs in Brazil

Cited by 22 publications

References 16 publications

Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri

Histopathological and functional changes in the testicles of albino rats experimentally infected with Arcobacter butzleri

Isolation of Arcobacter spp from the milk of dairy cows in Brazil

Arcobacter Population Dynamics in Pigs on Farrow-to-Finish Farms

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