Arabidopsis thaliana Cells: A Model to Evaluate the Virulence of Pectobacterium carotovorum

Abstract

Pectobacterium carotovorum are economically important plant pathogens that cause plant soft rot. These enterobacteria display high diversity world-wide. Their pathogenesis depends on production and secretion of virulence factors such as plant cell wall-degrading enzymes, type III effectors, a necrosis-inducing protein, and a secreted virulence factor from Xanthomonas spp., which are tightly regulated by quorum sensing. Pectobacterium carotovorum also present pathogen-associated molecular patterns that could participate in their pathogenicity. In this study, by using suspension cells of Arabidopsis thaliana, we correlate plant cell death and pectate lyase activities during coinfection with different P. carotovorum strains. When comparing soft rot symptoms induced on potato slices with pectate lyase activities and plant cell death observed during coculture with Arabidopsis thaliana cells, the order of strain virulence was found to be the same. Therefore, Arabidopsis thaliana cells could be an alternative tool to evaluate rapidly and efficiently the virulence of different P. carotovorum strains.

Curcumin from Curcuma longa affects the virulence of Pectobacterium wasabiae and P. carotovorum subsp. carotovorum via quorum sensing regulation

“…The use of phyto-compounds is novel and attractive when particularly these virulence factor inhibitors are non-toxic and have no adverse effect on the beneficial bacterial consortia of the host at the used concentration. Concomitantly, Terta et al (2010) reported that A. thaliana cells could be an alternative tool to evaluate the virulence of Pectobacterium spp. Moreover, they observed pathogenicity caused by Pectobacterium sp.…”

Deciphering the dual effect of lipopolysaccharides from plant pathogenicPectobacterium

“…40 An A. thaliana cell suspension was incubated (24 h) in their culture medium in presence of different concentrations of LPS with or without the appropriate pharmacological effectors. Then, cells (500 mL) were incubated for 10 min in 1 mL phosphate buffer (0.1 M -pH 7) supplemented with Evans Blue to a final concentration of 0.001%.…”

“…This type of cell death resembles the one detected during the early phase of infection of A. thaliana suspension cells with P. carotovorum. 40 Upon co-culture of plant cells with 10 8 cfu.mL ¡1 Pcc an early small increase in plant cell death occurred during the first 3 h before pectate-lyase activities were detectable, 40 with dead cells displaying plasmolysis. After 12 h and pectate lyase activity increase, plant cell death largely increased and cell integrity was not preserved ( 40 and Fig.…”

“…21 LPS from Pectobaterium were also shown to decrease Pcc-induced soft rot symptoms on potato tubers. 61 Since we have recently showed that A. thaliana cells could be used as a model to analyze Pcc virulence, 40 we checked if pretreatment with LPS from Pectobaterium or non-pathogenic bacteria could counteract the A. thaliana cell death induced by Pcc. As we previously reported, 40 the rate of A. thaliana cell death 8 cfu.mL ¡1 Pcc was 100% (Fig.…”

“…25 Variations in sugar composition of the OPS from Pectobcaterium species was used for differentiation into serogroup 37 while the OPS from P. atrosepticum present a specific higher branched monosaccharide. 38 In this study, using A. thaliana cell suspensions as a host model system, [39][40] we compared the effects of LPS from phytopathogenic bacteria, i.e. Pectobacterium carotovorum subsp carotovorum (Pcc) and Pa, and non-phytopathogenic bacteria i.e., Pseudomonas aeruginosa and Escherichia coli, on early cellular responses classically associated with defense responses and PCD.…”