Anti - Helicobacter pylori and antiulcerogenic activity of Aframomum pruinosum seeds on indomethacin-induced gastric ulcer in rats

Abstract

Context: Peptic ulcer is one of the most common diseases affecting mankind. Although there are many products used for its treatment, most of these products produce severe adverse reactions requiring the search for novel compounds. Some Afromomum species are used traditionally to cure acute gastritis.Objective: To evaluate the antiulcer activity of the methanol extract of Aframomum pruinosum Gagnepain (Zingiberaceae) seeds against two major etiologic agents of peptic ulcer disease; Helicobacter pylori and non-steroidal anti-inflammatory drugs.Materials and methods: The anti-Helicobacter activity of A. pruinosum was evaluated using the broth microdilution method. After oral administration of indomethacin (5 mg/kg) for 5 consecutive days, gastric ulcerated animals were divided into control group and five other groups: three groups that recieved respectively 125, 250 and 500 mg/kg of plant extract, the fourth group received Maalox (50 mg/kg) and the fifth group, Misoprostol (100 μg/kg), respectively, for 5 days. Ulcer areas, gastric mucus content and nitric oxide gastric levels of animals were assessed 24 h after this treatment.Results:A. pruinosum extract shows a moderate anti-Helicobacter activity with an MIC value of 128 μg/mL. A. pruinosum extract, like Misoprostol and Maalox, markedly reduces the % of ulcerated area from 8.15 ± 0.33 to 1.71 ± 0.44% (500 mg/kg). It also increased significantly mucus and NO gastric production with respective values of 4.44 ± 1.35 and 965.81 ± 106.74 μmol/g (500 mg/kg).Discussion and conclusion: These findings suggest that A. pruinosum methanol extract possesses antiulcer properties as ascertained by the comparative decreases in ulcer areas, increase of mucus and NO gastric production.

Broad spectrum resistance in Helicobacter pylori isolated from gastric biopsies of patients with dyspepsia in Cameroon and efflux-mediated multiresistance detection in MDR isolates

“…The inoculated culture media were incubated at 37 °C under microaerophilic conditions (CampyGen gas pack) for 7 days. Isolates that exhibited Gram negative curved rods on Gram stain reaction and were positive for catalase, oxidase and urease tests were considered as H. pylori [27, 28]. Confirmed isolates were suspended in eppendorf tubes containing Brain Heart Infusion broth supplemented with 5% horse serum (BHI-serum) with 30% glycerol and stored at − 80 °C until future use.…”

“…Subcultures were incubated at 37 °C under microaerophilic conditions (CampyGen gas pack) for 3 days and for two passages to ensure reliable growth. Gram staining, rapid urease and the catalase/oxydase tests were performed to confirm the identification [28, 29].…”

Antiulcer Agents: From Plant Extracts to Phytochemicals in Healing Promotion

“…and even other species from the Aframomun genus, are traditionally used in Cameroon to cure gastritis. A methanolic extract from A. pruinosum seeds (125, 250 and 500 mg/kg) was evaluated as antiulcerogenic agent using two different models, NSAID- and H. pylori -induced gastric lesion in rats [ 113 ]. This study concluded that the extract from this plant possessed a moderate anti- Helicobacter and antiulcer activities, with a cytoprotective action due to the increase in mucus production stimulated by the endogenous prostaglandins and nitric oxide generation mechanisms [ 113 ].…”

Effect of Celastrus orbiculatus in inhibiting Helicobacter pylori induced inflammatory response by regulating epithelial mesenchymal transition and targeting miR-21/PDCD4 signaling pathway in gastric epithelial cells

“…In recent years, Chinese herbal medicine has been shown to exhibit bacteriostatic effects on H. pylori [17]. Chang et al found that geniposide, a bioactive extract of Gardenia, could obviously reduce H. pylori infections by interfering with the growth and virulence as well as attenuating the inflammatory response [18].…”