An annotated catalogue of the hawkmoths (Sphingidae) of Rancho Laguna Blanca (RLB) is provided. A total of 63 species was recorded, about two-thirds of the Paraguay fauna, including formal documentation of five previously unrecorded species, which takes the country total to 95. The reserve is located in a transitional area from Atlantic Forest to Cerrado. This catalogue is the first published single site list for such a habitat, and the only single site list published for Paraguay to date that is based on year round sampling supported by voucher specimens. The importance of the RNLB for conservation at both the national and international level is highlighted.