<i>Agrobacterium-</i>mediated transformation of lettuce (<i>Lactuca sativa</i>L.) to express IgG-binding protein A and human pro-insulin as a fusion protein

Mohebodini, Mehdi; Jalali-Javaran, Mokhtar; Alizadeh, Houshang; Mahboudi, F; Yarbakht, Melina

doi:10.1080/14620316.2014.11513143

Cited by 11 publications

(6 citation statements)

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“…As a result, the percentage of recombinant protein in total protein of transgenic cucumber leaf tissue was about 0.01%.This low amount of recombinant protein production could be due to many reasons, such as recombinant protein degradation by plant protease or transfer red gene being satin the region of genome that has impossible or very low expression (PRASAD et al 2004, DORAN 2006.In various studies performed in transgenic plants,productconcentrationof0.01% to 0.1% of total protein and even less has been reported (DANIELL et al 2001). The amount of proinsulin protein in present study is about the same range as in other investigations, and this amount of expression is evaluated to be acceptable range of nuclear gene transformation (MOHEBODINI et al 2014).…”

Section: Electrochemiluminescencesupporting

confidence: 58%

“…Human Proinsulin gene cloning in plant expression binary vector pCAMBIA1304 were done as in (MOHEBODINI et al 2014). DNA of the binary vector (pCAMINS) was transferred into the A. tumefaciens strain LBA4404 by the freeze-thaw method.…”

Section: Bacterial Strains and Plasmid Constructmentioning

confidence: 99%

“…Nowadays, many biopharmaceutical proteins and peptides have been produced in transgenic plants (ERICKSON et al 2002). In more recent studies, the human insulin was expressed in transgenic Arabidopsis seeds (NYKIFORUK et al, 2006;MOHEBODINI et al, 2014). The Glucagon-like peptide-1 (GLP-1) was transformed into cucumbers and biological activity test results showed that the serum glucose level of diabetic rats was significantly decreased after oral administration of transgenic cucumber fruitage (ZHAO et al,.…”

Section: Introductionmentioning

confidence: 99%

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Transfer of human proinsulin gene into Cucumber (Cucumis sativus L.) via agrobacterium method

et al. 2017

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Nowadays, approximately 5.8% in adult population around the world are suffering by diabetes. It can be caused by an increase in risk factors such as being overweight. Also it has been estimated that the number of patients will be doubled in near future and the demands for insulin hormone will be growing up by 3 to 4 % annually. Therefore, it's necessary to develop new methods for hormone production with high rate of capacity in future. By advanced technology of transgenic DNA, the transgenic plants are introduced as an attractive system for expression and production of many kinds of pharmaceutical proteins. In this study, we investigated transfer of Human Proinsulin Gene into the Cucumber (Cucumissativus L.). Transgenic cucumber could be a great prospect for future source of eatable insulin pharmaceutical drugs to be taken by patients.Agrobacterium tumefaciensstrain LBA4404 carrying proinsulin genes with CaMV 35S promoter was used for the transformation purpose. The transgenic plants were analyzed by PCR, RT-PCR, SDS-PAGE, Dot blot and Electrochemiluminescence techniques. Production of proinsulin in cucumber could be a great prospect in molecular farming of human proinsulin.

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Section: Electrochemiluminescencesupporting

confidence: 58%

Section: Bacterial Strains and Plasmid Constructmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transfer of human proinsulin gene into Cucumber (Cucumis sativus L.) via agrobacterium method

et al. 2017

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“…In addition, the responses of callus induction and direct shoot regeneration on plant growth regulators were genotype‐dependent in Lactuca (Mohebodini et al, ). Roots were regenerated on medium with 0.05 mg/L NAA, and A. tumefaciens LBA4404 was used during transformation processes in Lactuca (Mohebodini, Jalali‐Javaran, Alizadeh, Mahboudi, & Yarbakht, ). In another study of L. sativa , the callus initiation medium contained 0.1 mg/L indole acetic acid (IAA) and 0.5 mg/L kinetin (KIN) (Michelmore, Marsh, Seely, & Landry, ).…”

Section: Discussionmentioning

confidence: 99%

Application of CRISPR/Cas9 to Tragopogon (Asteraceae), an evolutionary model for the study of polyploidy

Shan

Mavrodiev

et al. 2018

Molecular Ecology Resources

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Tragopogon (Asteraceae) is an excellent natural system for studies of recent polyploidy. Development of an efficient CRISPR/Cas9-based genome editing platform in Tragopogon will facilitate novel studies of the genetic consequences of polyploidy. Here, we report our initial results of developing CRISPR/Cas9 in Tragopogon. We have established a feasible tissue culture and transformation protocol for Tragopogon. Through protoplast transient assays, use of the TragCRISPR system (i.e. the CRISPR/Cas9 system adapted for Tragopogon) was capable of introducing site-specific mutations in Tragopogon protoplasts. Agrobacterium-mediated transformation with Cas9-sgRNA constructs targeting the phytoene desaturase gene (TraPDS) was implemented in this model polyploid system. Sequencing of PCR amplicons from the target regions indicated simultaneous mutations of two alleles and four alleles of TraPDS in albino shoots from Tragopogon porrifolius (2x) and Tragopogon mirus (4x), respectively. The average proportions of successfully transformed calli with the albino phenotype were 87% and 78% in the diploid and polyploid, respectively. This appears to be the first demonstration of CRISPR/Cas9-based genome editing in any naturally formed neopolyploid system. Although a more efficient tissue culture system should be developed in Tragopogon, application of a robust CRISPR/Cas9 system will permit unique studies of biased fractionation, the gene-balance hypothesis and cytonuclear interactions in polyploids. In addition, the CRISPR/Cas9 platform enables investigations of those genes involved in phenotypic changes in polyploids and will also facilitate novel functional biology studies in Asteraceae. Our workflow provides a guide for applying CRISPR/Cas9 to other nongenetic model plant systems.

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“…Such plants may be grouped into leaf-based and seed-based species. Leafy plants such as lettuce, are used as production platforms with moderately high expression levels: yields of 0.24% [6] and 0.13% [7] have been reported. Leafy non-food crops, such as varieties of tobacco with low nicotine and low alkaloid levels or alfalfa, are particularly suitable hosts because they are perennial plants with high biomass production [8].…”

Section: A "Stable" Expression Platform Using Agrobacterium-mediated mentioning

confidence: 99%

Development of Systems for the Production of Plant-Derived Biopharmaceuticals

Moon

Park

et al. 2019

Plants

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Over the last several decades, plants have been developed as a platform for the production of useful recombinant proteins due to a number of advantages, including rapid production and scalability, the ability to produce unique glycoforms, and the intrinsic safety of food crops. The expression methods used to produce target proteins are divided into stable and transient systems depending on applications that use whole plants or minimally processed forms. In the early stages of research, stable expression systems were mostly used; however, in recent years, transient expression systems have been preferred. The production of the plant itself, which produces recombinant proteins, is currently divided into two major approaches, open-field cultivation and closed-indoor systems. The latter encompasses such regimes as greenhouses, vertical farming units, cell bioreactors, and hydroponic systems. Various aspects of each system will be discussed in this review, which focuses mainly on practical examples and commercially feasible approaches.

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Agrobacterium-mediated transformation of lettuce (Lactuca sativaL.) to express IgG-binding protein A and human pro-insulin as a fusion protein

Cited by 11 publications

References 1 publication

Transfer of human proinsulin gene into Cucumber (Cucumis sativus L.) via agrobacterium method

Transfer of human proinsulin gene into Cucumber (Cucumis sativus L.) via agrobacterium method

Application of CRISPR/Cas9 to Tragopogon (Asteraceae), an evolutionary model for the study of polyploidy

Development of Systems for the Production of Plant-Derived Biopharmaceuticals

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