Transfer of human proinsulin gene into Cucumber (Cucumis sativus L.) via agrobacterium method

Abstract

Nowadays, approximately 5.8% in adult population around the world are suffering by diabetes. It can be caused by an increase in risk factors such as being overweight. Also it has been estimated that the number of patients will be doubled in near future and the demands for insulin hormone will be growing up by 3 to 4 % annually. Therefore, it's necessary to develop new methods for hormone production with high rate of capacity in future. By advanced technology of transgenic DNA, the transgenic plants are introduced as an attractive system for expression and production of many kinds of pharmaceutical proteins. In this study, we investigated transfer of Human Proinsulin Gene into the Cucumber (Cucumissativus L.). Transgenic cucumber could be a great prospect for future source of eatable insulin pharmaceutical drugs to be taken by patients.Agrobacterium tumefaciensstrain LBA4404 carrying proinsulin genes with CaMV 35S promoter was used for the transformation purpose. The transgenic plants were analyzed by PCR, RT-PCR, SDS-PAGE, Dot blot and Electrochemiluminescence techniques. Production of proinsulin in cucumber could be a great prospect in molecular farming of human proinsulin.

Synthetic biology-inspired cell engineering in diagnosis, treatment, and drug development

“…265 Recombinant human insulin has also been successfully produced in transgenic animals and plants, such as mice, 266 Arabidopsis thaliana, 267 and cucumber. 268 The production of monoclonal antibodies (mAbs) has taken tremendous attention in the pharmaceutical industry. A modular synthetic biology approach is proposed to rationally engineer E. coli according to three functional modules to facilitate high-titer production of immunoglobulin G (IgG).…”

Lipid Droplets from Plants and Microalgae: Characteristics, Extractions, and Applications

“…With a rapid increase in diabetic patients globally, the existing techniques for producing insulin using mammalian cells, Escherichia coli , and yeast expression systems will not be able to meet the growing demand for affordable insulin due to the limited production capacity and high production cost [ 124 , 125 , 126 , 127 ]. Therefore, it is essential to develop new expression systems for producing insulin.…”