Agave Leaves as a Substrate for the Production of Cellulases by Penicillium sp. and the Obtainment of Reducing Sugars

Silva-Mendoza, Julio; Gómez-Treviño, Alberto; López-Chuken, Ulrico Javier; Blanco-Gámez, Edgar Allan; Chávez-Guerrero, L.; Cantú-Cárdenas, María Elena

doi:10.1155/2020/6092165

Cited by 7 publications

(2 citation statements)

References 34 publications

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“…Endoglucanases (12.5 U/mL) and exoglucanases (7.8 U/mL) were also detected at conditions selected for optimal β -glucosidase production. Silva-Mendoza et al [ 22 ] also observed optimal endoglucanase and exoglucanase production in minimal media supplemented with wheat bran over a wide pH (4.0–6.0) and temperature (30–70 °C) range over longer periods (5 days).…”

Section: Resultsmentioning

confidence: 95%

Optimisation of β-Glucosidase Production in a Crude Aspergillus japonicus VIT-SB1 Cellulase Cocktail Using One Variable at a Time and Statistical Methods and its Application in Cellulose Hydrolysis

Singh

Sitholé

Govinden

2023

IJMS

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Pulp and paper mill sludge (PPMS) is currently disposed of into landfills which are reaching their maximum capacity. Valorisation of PPMS by enzymatic hydrolysis using cellulases is an alternative strategy. Existing commercial cellulases are expensive and contain low titres of β-glucosidases. In this study, β-glucosidase production was optimised by Aspergillus japonicus VIT-SB1 to obtain higher β-glucosidase titres using the One Variable at a Time (OVAT), Plackett Burman (PBD), and Box Behnken design (BBD)of experiments and the efficiency of the optimised cellulase cocktail to hydrolyse cellulose was tested. β-Glucosidase production was enhanced from 0.4 to 10.13 U/mL, representing a 25.3-fold increase in production levels after optimisation. The optimal BBD production conditions were 6 days of fermentation at 20 °C, 125 rpm, 1.75% soy peptone, and 1.25% wheat bran in (pH 6.0) buffer. The optimal pH for β-glucosidase activity in the crude cellulase cocktail was (pH 5.0) at 50 °C. Optimal cellulose hydrolysis using the crude cellulase cocktail occurred at longer incubation times, and higher substrate loads and enzyme doses. Cellulose hydrolysis with the A. japonicus VIT-SB1 cellulase cocktail and commercial cellulase cocktails resulted in glucose yields of 15.12 and 12.33 µmol/mL glucose, respectively. Supplementation of the commercial cellulase cocktail with 0.25 U/mg of β-glucosidase resulted in a 19.8% increase in glucose yield.

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Section: Resultsmentioning

confidence: 95%

Optimisation of β-Glucosidase Production in a Crude Aspergillus japonicus VIT-SB1 Cellulase Cocktail Using One Variable at a Time and Statistical Methods and its Application in Cellulose Hydrolysis

Singh

Sitholé

Govinden

2023

IJMS

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“…and Trichoderma sp. also have the ability to produce cellulases [18,[36][37][38][39], pectinase enzymes [40],and xylanase enzymes [41,42], which are all plant growth promoters.…”

Section: Growth Dynamics Of Fungal Strainsmentioning

confidence: 99%

Growth Stimulation, Phosphate Resolution, and Resistance to Fungal Pathogens of Some Endogenous Fungal Strains in the Rhizospheres of Medicinal Plants in Vietnam

Huong¹,

Hoài²,

Thao

et al. 2022

Molecules

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Endophytic fungi are recognized for their many potential applications in agriculture, such as supporting cropland expansion and increasing the yield and resistance of plants by creating antibiotics that inhibit the growth of pathogenic microorganisms. In addition, they can produce enzymes that break down hard-to-solubilize substances within soil, dissolve phosphates, fix nitrogen, reduce metals, and produce hormones that promote plant growth (auxin, cytokinin, and gibberellins) to keep crops healthy. In this report, three strains of endophytic fungi, namely, N1, N2, and N3, were isolated from the roots of Stevia rebaudiana (Bert.) Hemsl., Polyscias fruticosa, and Angelica dahurica in some localities in Vietnam. Through a screening process, it was found that they can produce high levels of indole acetic acid (IAA), resolve phosphates, and resist disease, and they were selected to as an alternative to chemical fertilizers to make probiotics in order to increase medicinal plant yields. The results show that the three strains of fungi have the ability to degrade phosphate to 341.90, 1498.46, and 390.79 ppm; the content of IAA produced in the culture medium reached 49.00, 52.35, and 33.34 ppm. Based on some morphological characteristics and an internal transcribed spacer gene sequence analysis of the fungal strains, N1, N2, and N3 were named Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3, respectively, which have the ability to inhibit the growth of pathogenic fungal strains, such as fungus C. gloeosporioides (CD1), fungus F. oxysporum, fungus L. theobromae N13, and N. dimidiatum. They grow significantly over a period of 5 to 6 days.

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Value Added Products from Agriculture, Paper and Food Waste: A Source of Bioenergy Production

Chandra

Srinivasulu

Yadav

et al. 2021

Clean Energy Production Technologies

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Agave Leaves as a Substrate for the Production of Cellulases by Penicillium sp. and the Obtainment of Reducing Sugars

Cited by 7 publications

References 34 publications

Optimisation of β-Glucosidase Production in a Crude Aspergillus japonicus VIT-SB1 Cellulase Cocktail Using One Variable at a Time and Statistical Methods and its Application in Cellulose Hydrolysis

Optimisation of β-Glucosidase Production in a Crude Aspergillus japonicus VIT-SB1 Cellulase Cocktail Using One Variable at a Time and Statistical Methods and its Application in Cellulose Hydrolysis

Growth Stimulation, Phosphate Resolution, and Resistance to Fungal Pathogens of Some Endogenous Fungal Strains in the Rhizospheres of Medicinal Plants in Vietnam

Value Added Products from Agriculture, Paper and Food Waste: A Source of Bioenergy Production

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