Growth Stimulation, Phosphate Resolution, and Resistance to Fungal Pathogens of Some Endogenous Fungal Strains in the Rhizospheres of Medicinal Plants in Vietnam

Abstract

Endophytic fungi are recognized for their many potential applications in agriculture, such as supporting cropland expansion and increasing the yield and resistance of plants by creating antibiotics that inhibit the growth of pathogenic microorganisms. In addition, they can produce enzymes that break down hard-to-solubilize substances within soil, dissolve phosphates, fix nitrogen, reduce metals, and produce hormones that promote plant growth (auxin, cytokinin, and gibberellins) to keep crops healthy. In this report, three strains of endophytic fungi, namely, N1, N2, and N3, were isolated from the roots of Stevia rebaudiana (Bert.) Hemsl., Polyscias fruticosa, and Angelica dahurica in some localities in Vietnam. Through a screening process, it was found that they can produce high levels of indole acetic acid (IAA), resolve phosphates, and resist disease, and they were selected to as an alternative to chemical fertilizers to make probiotics in order to increase medicinal plant yields. The results show that the three strains of fungi have the ability to degrade phosphate to 341.90, 1498.46, and 390.79 ppm; the content of IAA produced in the culture medium reached 49.00, 52.35, and 33.34 ppm. Based on some morphological characteristics and an internal transcribed spacer gene sequence analysis of the fungal strains, N1, N2, and N3 were named Penicillium simplicissimum CN7, Talaromyces flavus BC1, and Trichoderma konilangbra DL3, respectively, which have the ability to inhibit the growth of pathogenic fungal strains, such as fungus C. gloeosporioides (CD1), fungus F. oxysporum, fungus L. theobromae N13, and N. dimidiatum. They grow significantly over a period of 5 to 6 days.

Potential in enhancement of salinity tolerance to crops in Vietnam’s Mekong Delta using endophytes: A review

“…As regards, endophytes can be applied as bio-fertilizers or supplemental microbes in establishing agro-ecosystems [39,40]. However, endophyte has been a less explored area of research, there are few reports Vietnam [41][42][43][44][45]. A big data of microbes isolated from soil, sea, river, ect.…”

Recent advances in the use of Trichoderma-containing multicomponent microbial inoculants for pathogen control and plant growth promotion

“…In addition to conditioning for different adverse environmental effects, Trichoderma species also affect the development and growth of plants. The effect of Trichoderma strains on tomato (Macías-Rodríguez et al 2018;Wang et al 2021;Sehim et al 2023), cucumber (Liu et al 2021;Yedidia et al 2001), cotton (Silva et al 2022), as well as medicinal (Huong et al 2022) and ornamental plants (Andrzejak and Janowska 2022) has already been investigated in numerous studies. As a result of the Trichoderma treatments, an improvement in stem, leaf and root development, as well as increased amount of photosynthetic pigments was observed in several cases.…”

Isolation of endophytic fungi from Cotoneaster multiflorus and screening of drought-tolerant fungi and evaluation of their growth-promoting effects

“…IAA, as a class of endogenous auxins that can increase plant growth and promote plant root formation, plays a very important role in the process of plant growth and development (Kashyap et al, 2023). It can be found not only in plants but also in fungi (Spaepen and Vanderleyden, 2011), such as Huong et al (2022), who found that from stevia [Stevia rebaudiana (Bertoni) Bertoni] endophytic fungi, Penicillium simplicissimum CN7 and Talaromyces flavus BC1 separated from the root part and Trichoderma konilangbra DL3 have the ability to produce IAA. When, Chen et al (2011) studied the endophytic fungus of Rehmannia glutinosa (Gaert.)…”