Aeromonas hydrophilafrom marketed mullet (Mugil cephalus) in Egypt: PCR characterization ofβ-lactam resistance and virulence genes

Ramadan, Hazem; Ibrahim, Nermin A.; Samir, Mai; El-Moaty, Amany Abd; Gad, Tamer M.

doi:10.1111/jam.13734

Cited by 46 publications

(31 citation statements)

References 58 publications

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“…About 48·00% of the oyster‐borne Aeromonas species from a natural oyster bed was resistant to tetracycline, cephalothin, sulfamethoxazole‐trimethoprim, chloramphenicol and nalidixic acid (Evangelista‐Barreto et al ). Additionally, high resistance rates to ampicillin and cephalosporins have been reported in A. hydrophila isolates from marketed fish (Ramadan et al ). However, all mussel‐borne Aeromonas species showed susceptibility to high‐generation cephalosporins (cefotaxime and ceftriaxone), quinolones (ciprofloxacin and ofloxacin) and aminoglycosides (kanamycin and amikacin) in this study.…”

Section: Resultsmentioning

confidence: 99%

“…However, all mussel‐borne Aeromonas species showed susceptibility to high‐generation cephalosporins (cefotaxime and ceftriaxone), quinolones (ciprofloxacin and ofloxacin) and aminoglycosides (kanamycin and amikacin) in this study. Aeromonas strains are naturally susceptible to quinolones and aminoglycosides and the resistance has been less frequently observed (Janda and Abbott ; Ramadan et al ). Our results are in line with previous reports which implies that the bivalve‐borne aeromonads can exhibit multidrug resistance, although they are not periodically treated with antimicrobials (De Silva et al ; Dahanayake et al ).…”

Section: Resultsmentioning

confidence: 99%

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Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard‐shelled mussel ( Mytilus coruscus ) in Korea

Hossain

Wickramanayake

Dahanayake

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: Aeromonas sp. are known as common pathogenic bacteria isolated from seafood. The virulence factors and antimicrobial resistance profiles of mussel-borne Aeromonas sp. are poorly understood. This study demonstrated for the first time the existence of virulence markers and antimicrobial resistance of Aeromonas sp. from mussels in Korea. Majority of the isolates were positive for phenotypic virulence characteristics and harboured several virulence genes which reveal the potential virulence of mussel-borne Aeromonas sp. Multiple antimicrobial resistance was also observed among the isolates. Our study highlights the importance of food safety standards in mussel consumption. AbstractHard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. caviae (n = 3), A. allosaccharophila (n = 2) and A. bivalvium (n = 1) by gyrB gene sequencing. The sequence divergence between and within the species ranged from 3Á70 to 10Á40% and 0-1Á50% respectively. Every species formed a distinct group in a neighbour-joining phylogenetic tree. The DNase, gelatinase, caseinase, b-haemolysis, biofilm and lipase activities were observed in 33 (100Á00%), 31 (93Á93%), 30 (90Á90%), 27 (81Á81%), 21 (63Á63%) and 17 (51Á51%) isolates respectively. The virulence genes were detected by PCR in the following frequencies: fla (90Á09%), aer (87Á88%), hlyA (87Á88%), ahyB (81Á19%), gcaT (75Á76%), ser (69Á70%), lip (66Á67%), alt (57Á58%), ast (51Á51%) and act (21Á21%). Every isolate was resistant to at least three of 18 antimicrobials in the disk diffusion test. The multiple antimicrobial resistance index values ranged from 0Á11 to 0Á44 among the isolates. Our study suggests that mussels can be a potential reservoir of virulent and multidrugresistant Aeromonas sp.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard‐shelled mussel ( Mytilus coruscus ) in Korea

Hossain

Wickramanayake

Dahanayake

et al. 2020

Lett Appl Microbiol

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“…However, high resistance rates to ampicillin and cephalosporins with less resistance to aminoglycosides and quinolones have been documented in A. hydrophila isolated from marketed fish (Ramadan et al . ). The outcomes of our study together with these previous reports imply that the aeromonads from bivalves can show multidrug resistance, although they are not frequently treated with antibiotics.…”

Section: Discussionmentioning

confidence: 97%

Aeromonasspp. from marketed Yesso scallop (Patinopecten yessoensis): molecular characterization, phylogenetic analysis, virulence properties and antimicrobial susceptibility

et al. 2018

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“…Recently, the studies of antimicrobial profiles in Aeromonas has increased, due to the necessity of responsible use of antibiotics [181,182,[332][333][334][335][336][337]. Wimalasena et al [337] suggested that in relation to animals, pet turtles could be a health risk to humans, due to the antimicrobial resistant Aeromonas strains found in these animals.…”

Section: Antimicrobial Resistancementioning

confidence: 99%

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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The genus Aeromonas belongs to the Aeromonadaceae family and comprises a group of Gram-negative bacteria widely distributed in aquatic environments, with some species able to cause disease in humans, fish, and other aquatic animals. However, bacteria of this genus are isolated from many other habitats, environments, and food products. The taxonomy of this genus is complex when phenotypic identification methods are used because such methods might not correctly identify all the species. On the other hand, molecular methods have proven very reliable, such as using the sequences of concatenated housekeeping genes like gyrB and rpoD or comparing the genomes with the type strains using a genomic index, such as the average nucleotide identity (ANI) or in silico DNA–DNA hybridization (isDDH). So far, 36 species have been described in the genus Aeromonas of which at least 19 are considered emerging pathogens to humans, causing a broad spectrum of infections. Having said that, when classifying 1852 strains that have been reported in various recent clinical cases, 95.4% were identified as only four species: Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). Since aeromonads were first associated with human disease, gastroenteritis, bacteremia, and wound infections have dominated. The literature shows that the pathogenic potential of Aeromonas is considered multifactorial and the presence of several virulence factors allows these bacteria to adhere, invade, and destroy the host cells, overcoming the immune host response. Based on current information about the ecology, epidemiology, and pathogenicity of the genus Aeromonas, we should assume that the infections these bacteria produce will remain a great health problem in the future. The ubiquitous distribution of these bacteria and the increasing elderly population, to whom these bacteria are an opportunistic pathogen, will facilitate this problem. In addition, using data from outbreak studies, it has been recognized that in cases of diarrhea, the infective dose of Aeromonas is relatively low. These poorly known bacteria should therefore be considered similarly as enteropathogens like Salmonella and Campylobacter.

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Aeromonas hydrophilafrom marketed mullet (Mugil cephalus) in Egypt: PCR characterization ofβ-lactam resistance and virulence genes

Abstract: These data provide insights into the potential association of antimicrobial resistance and virulence genes in A. hydrophila from marketed mullet in Egypt, which could pose threats to humans even if a weak positive correlation exists between both genes.

Cited by 46 publications

References 58 publications

Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard‐shelled mussel ( Mytilus coruscus ) in Korea

Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard‐shelled mussel ( Mytilus coruscus ) in Korea

Aeromonasspp. from marketed Yesso scallop (Patinopecten yessoensis): molecular characterization, phylogenetic analysis, virulence properties and antimicrobial susceptibility

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

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