Achyrocline satureioides(Lam.) D.C. Hydroalcoholic Extract Inhibits Neutrophil Functions Related to Innate Host Defense

Abstract

Achyrocline satureioides (Lam.) D.C. is a herb native to South America, and its inflorescences are popularly employed to treat inflammatory diseases. Here, the effects of the in vivo actions of the hydroalcoholic extract obtained from inflorescences of A. satureioides on neutrophil trafficking into inflamed tissue were investigated. Male Wistar rats were orally treated with A. satureioides extract, and inflammation was induced one hour later by lipopolysaccharide injection into the subcutaneous tissue. The number of leukocytes and the amount of chemotactic mediators were quantified in the inflammatory exudate, and adhesion molecule and toll-like receptor 4 (TLR-4) expressions and phorbol-myristate-acetate- (PMA-) stimulated oxidative burst were quantified in circulating neutrophils. Leukocyte-endothelial interactions were quantified in the mesentery tissue. Enzymes and tissue morphology of the liver and kidney were evaluated. Treatment with A. satureioides extract reduced neutrophil influx and secretion of leukotriene B4 and CINC-1 in the exudates, the number of rolling and adhered leukocytes in the mesentery postcapillary venules, neutrophil L-selectin, β2-integrin and TLR-4 expression, and oxidative burst, but did not cause an alteration in the morphology and activities of liver and kidney. Together, the data show that A. satureioides extract inhibits neutrophil functions related to the innate response and does not cause systemic toxicity.

Effect of the treatment with Achyrocline satureioides (free and nanocapsules essential oil) and diminazene aceturate on hematological and biochemical parameters in rats infected by Trypanosoma evansi

“…kidney without cellular alterations (Baldissera et al, 2014c). In animals treated with hydroalcoholic extract, A. satureioides did not change the levels of creatinine and urea (Barioni et al, 2013). According to Javaid et al (2012), the A. satureioides is considered in literature as a potent nephroprotective plant.…”

Hydroalcoholic Extract from Inflorescences of Achyrocline satureioides (Compositae) Ameliorates Dextran Sulphate Sodium‐Induced Colitis in Mice by Attenuation in the Production of Inflammatory Cytokines and Oxidative Mediators

“…More particularly, there are several reports indicating that tea prepared mainly by communities of South Brazil, from the inflorescences of A. satureioides , leads to the relief of the symptoms of gastric ulcers [10, 11] and inflammatory diseases of the gastrointestinal tract, such as Crohn's disease [11, 12]. In line with its traditional use, the anti-inflammatory activity of extracts from A. satureioides has been confirmed in different studies [11, 13, 14]. Santin et al [15] previously confirmed the antiulcer gastric potential of this plant and its antispasmodic effect in gastrointestinal smooth muscle preparation has also been described [13].…”

“…However, despite these findings and popular usage to treat IBD, no reports are available about the intestinal anti-inflammatory effects A. satureioides preparations. Therefore, the present study has been designed to examine the intestinal anti-inflammatory effects of the hydroalcoholic extract of inflorescences of A. satureioides (HEAS), which has already been chemically characterized being the major components identified as quercetin and luteolin [14], in dextran sulfate sodium (DSS) induced colitis in mice.…”

Anti-Inflammatory and Healing Potential of Phytoderivatives in Cutaneous Rabbits Wounds

“…[21,48] It is a plant that works to reduce the influx of neutrophils and secretion of leukotriene B4 at the site of inflammation, blocking the expression/activity of molecules involved in the interaction between immunocirculating cells and endothelial cells, reducing the body's innate response without causing systemic toxicity. [49] Aqueous and ethanolic extracts of A. satureioides promote proliferation of keratinocyte and human lung fibroblasts lines cells mainly at low concentrations without showing cytotoxicity at higher concentrations in vitro [50] reinforcing its popular use for wound healing by acting in different stages of the repair process of injured tissues and in intensified inflammatory processes, this bio-activity is an important therapeutic strategy.…”