<i>Achyranthes aspera</i>Root Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest

Arora, Shagun; Tandon, Simran

doi:10.1155/2014/129697

Cited by 23 publications

(19 citation statements)

References 31 publications

(30 reference statements)

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“…Bufalin was shown to be potent against human OS tissues and cell lines via inducing apoptosis (20,21); however, the specific underlying mechanism has remained elusive. Mitochondria-mediated signaling pathways have a pivotal role in the process of apoptosis (22)(23)(24)(25), and have been identified to be the underlying mechanism of bufalin-induced apoptosis in various types of tumor cell (21). As the specific mechanisms of action of bufalin vary between different tumor types or even between different phenotypes of the same tumor type, the mechanisms of the effects of bufalin on OS cell lines require to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Bufalin induces apoptosis in the U-2OS human osteosarcoma cell line via triggering the mitochondrial pathway

Chen¹,

Li²,

Li³

et al. 2015

Molecular Medicine Reports

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Abstract. Bufalin has been shown to induce apoptosis in osteosarcoma cells; however, the underlying mechanism has not been elucidated. The purpose of the present study was to investigate whether mitochondria-mediated signaling pathways trigger the process of apoptosis in the U-2OS osteosarcoma cell line. Bufalin inhibited the proliferation and induced apoptosis in U-2OS cells in a time-and dose-dependent manner. Bufalin-induced apoptosis was accompanied with a significant reduction of the mitochondrial membrane potential, release of mitochondrial cytochrome c into the cytosol, activation of caspase-3, caspase-9 and poly(adenosine diphosphate ribose) polymerase, as well as downregulation of B-cell lymphoma 2 (Bcl-2)/Bcl-2-associated X protein. Cyclosporin A, a specific inhibitor of the mitochondrial permeability transition pore, attenuated bufalin-induced apoptosis. In conclusion, the present study revealed that bufalin induced apoptosis in the U-2OS human osteosarcoma cell line via triggering of the mitochondrial pathway.

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Section: Introductionmentioning

confidence: 99%

Bufalin induces apoptosis in the U-2OS human osteosarcoma cell line via triggering the mitochondrial pathway

Chen¹,

Li²,

Li³

et al. 2015

Molecular Medicine Reports

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“…For a cell to be able to divide into two daughter cells, the synthesis and duplication of the DNA are a requirement, and any abnormality in this would lead to an obstacle in cell cycle progression. Numerous checkpoints exist to aid cells in the identification and repair of DNA damage by halting/stalling the progression through the phases of the cell cycle, including the G 0 /G 1 , S and G 2 /M checkpoints (40). The current study observed arrest at the S to G 2 /M phase transition of the cell cycle upon TCS treatment, leading to a halt in cell cycle progression.…”

mentioning

confidence: 49%

“…important target in cancer therapy (40). G 1 phase arrest by TCS has been previously reported in breast cancer cells (7) and A549 lung cancer cells (41).…”

mentioning

confidence: 92%

Trichosanthin reduces the viability of SU-DHL-2 cells via the activation of the extrinsic and intrinsic apoptotic pathways

Zhu

Sun

Cai

et al. 2015

Molecular Medicine Reports

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Previous studies have indicated that trichosanthin (TCS) exerts antitumor activity by inducing apoptosis in numerous tumor cell lines. However, the effects of TCS on lymphoma remain to be elucidated. The current study demonstrated that TCS inhibited the proliferation of thirteen lymphoma cell lines in a dose‑dependent manner, with SU‑DHL‑2 cells exhibiting the greatest sensitivity to TCS. Treatment of SU‑DHL‑2 cells with TCS led to cell cycle arrest at the S to G2/M phase transition. Furthermore, flow cytometric analysis, Hoechst 33258 staining and western blotting indicated that TCS induced the apoptosis of SU‑DHL‑2 cells in a time‑ and concentration‑dependent manner. In addition, the activation of caspase‑3 and ‑7 and poly (ADP‑ribose) polymerase were observed. Pharmacological pan-caspase inhibition was observed to reduce TCS‑induced apoptosis. Inhibition of caspase‑8 or ‑9 alone was observed to partially reverse the effect of TCS on apoptosis. In conclusion, the current study indicates that TCS may induce apoptosis in SU‑DHL‑2 cells via the extrinsic and intrinsic pathways.

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“…Several workers reported chromatin condensation and DNA fragmentation of different cancer cells by different mushroom extracts such as EAC extract (10 g/ml) of Cyathus striatus treatment on HPAF-II and PL45 cells 41 , the extract of Agaricus blazei against leukemia NB-4 and K-562 cells 42 , and extract of A. bisporus on CaSki cells after 48 h treatment 38 . Treatment of COLO-205 cells with of three mushrooms (Auricularia polytricha, Macrolepiota procera and Pleurotus ostreatus) revealed the characteristic pattern of DNA laddering on agarose gel electrophoresis 43 . Our DNA laddering assay result is corroborated with other's results.…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer property of Lenzites betulina (L) Fr. on cervical cancer cell lines and its anti-tumor effect on HeLa-implanted mice

Sanyal

Ghosh

2019

Preprint

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In global scenario cervical cancer is increasing. New drugs from natural compounds are in search. Mushrooms are now recognized as miniature pharmaceutical factories producing hundreds of novel constituents. We have taken ethanolic extract Lenzities betulina (LBE) wild mushroom for evaluation of its as anti-cancer property against cervical cancer cell lines e.g. HeLa, CaSki and SiHa and anti tumor activity against HeLa implanted tumor on mice. The extraction was done by dip and stirring method in 90% ethanol for 72 h. For evaluation of anti-cervical cancer, several assays were performed such as MTT assay, cell morphology by phase contrast microscope and F-action polymerization by Laser scanning confocal microscope and nuclear morphology DAPI staining under inverted fluorescence microscope, MMP, ROS, cell cycle, autophagy and stem cell population by flow cytometry and DNA laddering were done. Western blotting was done for protein expression. To evaluate anti-metastatic activity, anti-cologenic assay and wound healing assay were adopted. For chemo-analysis of the LBE, GC-MS was done. The results from Cytotoxicity assay showed that at highest dose of LBE (1000 µg/ml) after 24 h, percentage of cell inhibitions were 85.13 %, 77.13 % and 47.70 % against HeLa , CaSki and SiHa respectively and the calculated IC 50 values were 492.52 ± 2.6 µg/ml, 612.22 ± 4.2 µg/ml, and 1210.30 ± 6.4 µg/ml respectively . Depending upon the cytotoxicity screening, HeLa cell line was considered for the further studies. Cell morphology study exhibited that LBE treated HeLa cells became round from normal spindle shape. DAPI staining showed that LBE treated nucleus became condensed and fragmented. DNA fragmentation at 230 and 300 base pair zone from agarose gel assay was observed. LBE induced ROS generation and reduced MMP. It up regulated the expression of apoptotic genes and p53 while down regulated Bcl2, pro-caspase 3 and pro caspase-9 gene. Cell cycle was arrested at G2/M checkpoint. Autophagic induction was exhibited by vacuole formation in treated cells. CSC population of treated cells was reduced and F-actin polymerization was observed in treated cells. In addition, LBE suppressed metastatic nature by inhibition of cell migration and colonization. The inhibition of growth of the tumors in HeLa cell-implanted mice showed that treatment with 50 mg LBE/kg of body weight of mice led to a marked reduction in the volume (93.22 ± 9.2 %) and weight (90.42 ±9.55 %) of the tumors. The GC-MS profile of LBE shows that out of 69 compounds, 9, 12-Octadecadienoic acid (Z, Z) and 8, (3.beta22E) are in a significantly higher proportion with the percentage peak area 22.13 and 19.72 respectively. Library search for bioactivity showed that these compounds are anti-cancerous and interestingly 4'-Hydroxy-6methoxyaurone binding with P-glycoprotein inhibits the cancer cells to become drug resistant. In conclusion, LBE is very prominent anti-cervical cancer having a lot of anti-cancerous compounds which are probably acting synergistically. This report of anti-ce...

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Achyranthes asperaRoot Extracts Induce Human Colon Cancer Cell (COLO-205) Death by Triggering the Mitochondrial Apoptosis Pathway and S Phase Cell Cycle Arrest

Cited by 23 publications

References 31 publications

Bufalin induces apoptosis in the U-2OS human osteosarcoma cell line via triggering the mitochondrial pathway

Bufalin induces apoptosis in the U-2OS human osteosarcoma cell line via triggering the mitochondrial pathway

Trichosanthin reduces the viability of SU-DHL-2 cells via the activation of the extrinsic and intrinsic apoptotic pathways

Anti-cancer property of Lenzites betulina (L) Fr. on cervical cancer cell lines and its anti-tumor effect on HeLa-implanted mice

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