<i>A Priori</i> Dietary Ω‐3 Lipid Supplementation Results in Local Pancreatic Macrophage and Pulmonary Inflammatory Response Attenuation in a Model of Experimental Acute Edematous Pancreatitis (AEP)

Sharif, Suhail; Broman, Michael; Babcock, Tricia A.; Ong, Evan; Jho, David H.; Rudnicki, Marek; Helton, W. Scott; Espat, N. Joseph

doi:10.1177/0148607106030004271

Cited by 8 publications

(8 citation statements)

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“…Our data demonstrate that the increased n-3 PUFA tissue content also lowers neutrophil infiltration in the lung and thus show a lowered pulmonary inflammatory reaction as a further sign of decreased systemic inflammation and multiorgan involvement. These results are consistent with previous studies showing beneficial effects of n-3 PUFA on acute pancreatitis [25–27]. …”

Section: Discussionsupporting

confidence: 93%

“…These data now need verification in other animal models as well as in the context of human disease. Together with the previous animal [25–27], as well as human studies [43, 44] examining acute pancreatitis and n-3 PUFA supplementation, our data support a potential benefit for n-3 PUFA supplementation particularly in chronic pancreatitis.…”

Section: Discussionsupporting

confidence: 85%

“…Our previous results in the fat-1 mouse model showed that an increased tissue status of n-3 PUFA led to formation of resolvins and protectins and to protection from acute DSS induced colitis [24]. Recent studies have implicated a role of n-3 PUFA in the dampening of acute pancreatic inflammation [25–28]. …”

Section: Introductionmentioning

confidence: 99%

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Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis

Weylandt

Nadolny

Kahlke

et al. 2008

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Summary Pancreatitis is a severe debilitating disease with high morbidity and mortality. Treatment is mostly supportive, and until now there are no clinically useful strategies for anti-inflammatory therapy. Although omega-3 polyunsaturated fatty acids (n-3 PUFA) are known to have anti-inflammatory effects, the utility of these fatty acids in the alleviation of pancreatitis remained to be investigated. The aim of this study was to examine the effect of n-3 PUFA on both acute and chronic pancreatitis in a well-controlled experimental system. We used the fat-1 transgenic mouse model, characterised by endogenously increased tissue levels of n-3 PUFA, and their wild-type littermates to examine the effect of n-3 PUFA on both acute and chronic cerulein induced pancreatitis. Disease activity and inflammatory status were assessed by both histology and molecular methods. In acute pancreatitis, fat-1 mice showed a trend towards decreased necrosis and significantly reduced levels of plasma IL-6 levels as well as reduced neutrophil infiltration in the lung. In chronic pancreatitis there was less pancreatic fibrosis and collagen content accompanied by decreased pancreatic stellate cell activation in the fat-1 animals with increased n-3 PUFA tissue levels as compared to wild-type littermates with high levels of omega-6 (n-6) PUFA in their tissues. Our data provide evidence for a reduction of systemic inflammation in acute pancreatitis and of tissue fibrosis in chronic pancreatitis by increasing the tissue content of omega-3 polyunsaturated fatty acids. These results suggest a beneficial potential for n-3 PUFA supplementation in acute and particularly chronic pancreatitis.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 85%

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Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis

Weylandt

Nadolny

Kahlke

et al. 2008

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…For example, Sharif et al have shown that oral gavage of X-3FAs to Spraque-Dawley (SD) rats lowered the macrophage infiltration in acute edematous pancreatitis [48]. In the same animal model, parenteral X-3FAs supplementation with fish oil increased the anti-inflammatory cytokine IL-10 and improved renal and respiratory function with acute pancreatitis [49].…”

Section: Discussionmentioning

confidence: 99%

Acute Pancreatitis in Obesity: Adipokines and Dietary Fish Oil

et al. 2011

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“…Sections (3 mm thick) were cut, dewaxed and stained with HE for histological examination using a BX-5D1TF microscope (Olympus, Tokyo, Japan). The pathological scores of pancreatitis ( 10 ) and lung tissue samples ( 11 ) were evaluated. For the pancreatic tissue samples, a scale of 0–4 was used for interstitial edema and hemorrhage, inflammatory cell infiltration and acinar cell vacuolization and necrosis, consistent with the improved scoring method, described by Sharif et al ( 10 ).…”

Section: Methodsmentioning

confidence: 99%

Efficacy of thymosin α1 and interferon α for the treatment of severe acute pancreatitis in a rat model

Wang

Zeng

Yang

et al. 2015

Molecular Medicine Reports

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The present study aimed to investigate the effects of treatment with thymosin α1 (TA1) or interferon α (IFNα) following the establishment of severe acute pancreatitis (SAP) in rats. A total of 144 Sprague-Dawley rats were randomly divided into four groups. The rats in all four groups were celiotomized, and the rats in the control group were administered with an intravenous injection of saline. The three other groups were administered with 5% 1 ml/kg sodium taurocholate via the cholangiopancreatic duct. SAP group rats were administered with an intravenous injection of saline; TA1 group rats received 26.7 µg/kg TA1; and interferon α (INFα) group rats received 4.0×105 U/kg IFNα. The rats were anesthetized and blood samples were collected from the animals 3, 12 and 24 h after surgery. The levels of T cell subsets, serum enzyme indicators, cytokines and procalcitonin (PCT) were measured. The general conditions of the rats were observed until sacrifice, and pancreatic and lung tissue samples were sampled for hematoxylin and eosin staining and histological scoring. The expression levels of aspartate transaminase, lactate dehydrogenase, α-amylase (AMY), P-type-amylase, lipase, PCT, tumor-necrosis factor α, interleukin (IL)-4, IL-5, and IL-18 in the TA1 and IFNα-treated rats were significantly lower, compared with those of the SAP rats within the first 24 h of model establishment (P<0.05). The TA1 and IFNα-treated rats exhibited significantly increased levels of CD3+, CD4+ and CD8+ T cells, and an increased ratio of CD4+/CD8+ cells, compared with SAP rats. Histological analysis revealed that the TA1 and IFNα-treated rats exhibited significantly ameliorated pancreas and lung damage, and mortality rates were reduced from 50.0% (6/12) to 25.0% (3/12) and 33.3% (4/12), respectively. The immunomodulatory agents TA1 and IFNα reduced acute inflammation, decreasing cell damage and enhancing immune function and survival rates in the SAP rats.

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A Priori Dietary Ω‐3 Lipid Supplementation Results in Local Pancreatic Macrophage and Pulmonary Inflammatory Response Attenuation in a Model of Experimental Acute Edematous Pancreatitis (AEP)

Abstract: Attenuation of both pancreatic MPhi inflammatory response and pulmonary leukocyte respiratory burst in AEP by EPA supports further investigation into the potential role for EPA dietary supplementation in the progression of pancreatitis-associated sequelae.

Cited by 8 publications

References 10 publications

Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis

Reduction of inflammation and chronic tissue damage by omega-3 fatty acids in fat-1 transgenic mice with pancreatitis

Acute Pancreatitis in Obesity: Adipokines and Dietary Fish Oil

Efficacy of thymosin α1 and interferon α for the treatment of severe acute pancreatitis in a rat model

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