Hypoxia-inducible factor 1α in the central nervous system of the scallop Mizuhopecten yessoensis Jay, 1857 (Bivalvia: Pectinidae) during anoxia and elevated temperatures

Kotsyuba, E. P.

doi:10.1134/s1063074017040071

Cited by 5 publications

(6 citation statements)

References 43 publications

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“…The HIF-1 signaling pathway was significantly enriched in the Manila clam and small abalone under hypoxia stress (Sun et al, 2019;. In the Yesso scallop, Kotsyuba reported that the activity of HIF-1a depended on the duration of anoxia and the temperature (Kotsyuba, 2017). In the present study, the HIF-1 signaling pathway was significantly enriched for the upregulated DEGs under severe heat and hypoxia.…”

Section: Hif-1 Signaling Pathway Mediates Severe Heat and Hypoxia Stresssupporting

confidence: 54%

Transcriptome analysis provides insight into adaptive mechanisms of scallops under environmental stress

et al. 2022

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High temperature and hypoxia greatly threaten marine life and aquaculture. Scallops, a diverse and ecologically important group of high economic value, mostly thrive in fluctuating environments, and are vulnerable to environmental stress. In the present study, the molecular response mechanism of scallops to a combination of environmental stressors was investigated via transcriptome analysis of the gill tissues in three scallop species, the Yesso scallop (Patinopecten yessoensis), Zhikong scallop (Chlamys farreri) and bay scallop (Argopecten irradians) that were exposed to transient heat, hypoxia and a combination thereof. The Yesso scallop had the most differentially expressed genes (DEGs) compared with the other two scallop species, indicating the highest sensitivity of the Yesso scallop to environmental stress. With increased temperature and decreased dissolved oxygen, the number of DEGs was greatly increased in the three scallop species, indicative of the enhancement in gene expression regulation in scallops in response to severe environmental changes. Heat and hypoxia had a synergistic effect on scallops. GO and KEGG enrichment analysis of DEGs under different stressors revealed overlapping molecular mechanisms of response in scallops following exposure to heat and hypoxia. Several immune and apoptosis-related pathways were highly enriched in the upregulated DEGs of the three scallops, suggesting that immune system activation and apoptosis promotion occurred in scallops in response to environmental stress. Heat shock proteins (HSPs) were significantly upregulated under heat and hypoxia, which likely assisted in correct protein folding to facilitate the adaption of the scallops to the altered environment. Additionally, the HIF-1 signaling pathway—the key pathway associated with hypoxia response—was triggered by extremely acute environmental changes. Comparative transcriptome analysis revealed 239 positively selected genes among the different scallops, including those involved in immune system and environmental adaptation, suggesting a long-term mechanism of environmental adaptation. The present study provides new insights into the molecular response mechanism in scallops to multiple environmental stressors and improves our understanding of the adaptive mechanisms of marine organisms under changing global climate conditions.

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Section: Hif-1 Signaling Pathway Mediates Severe Heat and Hypoxia Stresssupporting

confidence: 54%

Transcriptome analysis provides insight into adaptive mechanisms of scallops under environmental stress

et al. 2022

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“…Hypoxia inducible factors (HIFs) play regulatory functions in these responses, and their mechanism of regulation is well understood in terrestrial organisms (Gorr et al, 2006; Ke and Costa, 2006; Wang et al, 1995). The regulation of physiological responses by HIFs was also observed in marine animals such as bivalves, crustaceans and fish (Kotsyuba, 2017; Piontkivska et al, 2011; Soitamo et al, 2001; Li and Brouwer, 2007). On the other hand, the behavioral responses include avoidance of hypoxic water (Eby and Crowder, 2002; Wu et al, 2002) and suppression of heartbeat rates (Eby and Crowder, 2002; Marshall and McQuaid, 1993).…”

Section: Introductionmentioning

confidence: 87%

Identification of possible hypoxia sensor for behavioral responses in a marine annelid, Capitella teleta

Ogino

Toyohara

2019

Biology Open

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Hypoxia often occurs in summer and causes deleterious effects on marine benthic animals. A marine annelid, Capitella teleta , is tolerant to hypoxia, as shown by the fact that it inhabits organically polluted areas, where severe hypoxia is often observed. To understand how this species adapts to the environment, we focused on its hypoxia sensor, and we showed that TRPAbasal was a possible contributor to hypoxia detection in C. teleta . To examine the involvement of TRPA1 in the response of C. teleta to hypoxia, we exposed C. teleta to hypoxic water with or without a TRPA1-specific inhibitor, A-967079. Hypoxic stimulation induced escape behavior in C. teleta from the sediment, and this behavior was suppressed by the inhibitor. The cloned TRPA gene from C. teleta was phylogenetically categorized into TRPAbasal , and contains an oxygen-dependent degradation domain, which is important for the detection of hypoxia. Whole-mount in situ hybridization analysis showed that the gene was transcribed in the prostomium, where sensing functions are localized. These results suggested that the worm has a hypoxia-sensing system possibly utilizing CtTRPAbasal, and this system contributes to expanding the organism's niches in hypoxic environments by detecting whether hypoxia exceeds a level that would imperil its survival.

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“…The mRNA expression level of HIF‐1α was analysed using qRT‐PCR assays in the Step One Plus Real‐Time PCR (Applied Biosystems, USA) using SYBR® Premix Ex Taq™ kit (Takara, Japan) (Kotsyuba, 2017). Each sample was triplicate for q RT‐PCR analyses.…”

Section: Methodsmentioning

confidence: 99%

“…HIF‐1α regulates the expression of genes involved in oxygen metabolism in response to hypoxic conditions (Karamjit et al, 2007). The hypoxia‐mediated molecular mechanism of the HIF‐1α genes has been revealed in marine molluscs, including the Pacific oysters Crassostrea gigas (Kawabe & Yokoyama, 2012; Piontkivska et al, 2011), the scallop Mizuhopecten yessoensis (Kotsyuba, 2017), abalone Haliotis discus hannai (Kim et al, 2021) and Haliotis diversicolor (Cai et al, 2014). Unlike many marine molluscs, clams are usually distributed over a wide spatial range in coastal tidal mudflats where hypoxia frequently occurs (Ge et al, 2022; Kawabe & Yokoyama, 2012; Ni et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Ni¹,

Wang²,

Xie³

et al. 2022

Aquaculture Research

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Aquatic hypoxia has negative or detrimental effects on clams. To cope with hypoxia stress, a complex series of physiological and biochemical strategies can be employed in clams. Hypoxia‐inducible factor‐1α (HIF‐1α) and antioxidant enzymes are commonly found in aquatic animals as an adaptation against hypoxia. In the present study, we cloned the HIF‐1α sequence in clam Cyclina sinensis, and it consists of 2654 base pairs (bp) carrying a single open‐reading frame that encompasses 2235 bp of the coding region. The predicted C. sinensis HIF‐1α protein (744 amino acids) has the conserved HLH‐PAS domain, which contained one N‐terminal basic helix–loop–helix (bHLH) domain and two Per‐ARNT‐Sim A/B (PAS) domains, followed by PAS‐associated C‐terminal (PAC) domain. HIF‐1α is expressed predominantly in gills. Exposed to hypoxia, the transcript level of HIF‐1α was significantly up‐regulated in response to hypoxia, and then, the transcript level declined continuously with the time elapsed. The antioxidant enzymes (SOD, CAT and GSH‐PX) in gills were increased initially and then decreased after acute hypoxia exposure. Our study indicates that the HIF‐1α gene and antioxidant enzymes may play an important role in cope with acute hypoxia exposure.

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Hypoxia-inducible factor 1α in the central nervous system of the scallop Mizuhopecten yessoensis Jay, 1857 (Bivalvia: Pectinidae) during anoxia and elevated temperatures

Cited by 5 publications

References 43 publications

Transcriptome analysis provides insight into adaptive mechanisms of scallops under environmental stress

Transcriptome analysis provides insight into adaptive mechanisms of scallops under environmental stress

Identification of possible hypoxia sensor for behavioral responses in a marine annelid, Capitella teleta

Unravelling the characterization of hypoxia‐inducible factor‐1α ( HIF‐1α ) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

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