2018
DOI: 10.3892/mmr.2018.9644
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Hypoxia‑induced miR‑210 contributes to apoptosis of mouse spermatocyte GC‑2 cells by targeting Kruppel‑like factor 7

Abstract: The aim of the present study was to investigate the underlying mechanisms of hypoxia-induced microRNA (miR)-210 effects on mouse GC-2spd (GC-2) cells. GC-2 cells were subjected to hypoxia or normoxia for 12, 24, 48 and 72 h. Apoptosis of GC-2 cells was detected using terminal deoxynucleotidyl-transferase-meditated dUTP nick end labeling and flow cytometry. Reverse transcription-quantitative polymerase chain reaction was performed to analyze the expression of miR-210. Hypoxia-inducible factor-1α (HIF-1α), caspa… Show more

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Cited by 16 publications
(21 citation statements)
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References 40 publications
(38 reference statements)
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“…Blockage of hypoxia-induced reduction of GR protein by LNA-anti-microRNA-210 alleviated cardiomyocyte apoptosis [5]. A recent study has shown that hypoxia-induced microRNA-210 promoted apoptosis of mouse spermatocyte GC-2 cells by directly targeting Kruppel-like factor 7 [54]. Our present study demonstrated that hypoxia significantly upregulated microRNA-210 expression in Sca-1 + cardiac cells and increased Sca-1+ cell proliferation.…”
Section: Discussionsupporting
confidence: 62%
“…Blockage of hypoxia-induced reduction of GR protein by LNA-anti-microRNA-210 alleviated cardiomyocyte apoptosis [5]. A recent study has shown that hypoxia-induced microRNA-210 promoted apoptosis of mouse spermatocyte GC-2 cells by directly targeting Kruppel-like factor 7 [54]. Our present study demonstrated that hypoxia significantly upregulated microRNA-210 expression in Sca-1 + cardiac cells and increased Sca-1+ cell proliferation.…”
Section: Discussionsupporting
confidence: 62%
“…Growing evidence suggests that miRNAs play an epigenetic role in many physiological processes through regulating multiple target genes in post‐transcriptional process, including development, cell proliferation (Clotaire, Du, Wei, Yang, & Hua, ), differentiation (Gao et al, ) and apoptosis (Capra et al, ). Several miRNAs have been identified in spermatozoa that have implications as critical regulators of apoptosis (Capra et al, ; Dai et al, ; Lv et al, ; Ma et al, ). Apoptosis regulates spermatogenesis (Wang, Li, Gong, Zhao, & Wu, ) and spontaneous programmed cell death in mature spermatozoa (Aitken & Baker, ; Engel, Springsguth, & Grunewald, ).…”
Section: Discussionmentioning
confidence: 99%
“…These effects were eliminated by the knockdown of miR-210. Studies have found that miR-210 has other targets including PLK1, ISCU, Toll-like receptor, and Kruppel-like factor 7 [32][33][34][35]. Expanding on this data, we identified AIFM3 as a novel target of miR-210.…”
Section: Discussionmentioning
confidence: 59%