Hypoxanthine Biosensor Based on Immobilization of Xanthine Oxidase on Modified Pt Electrode and Its Application for Fish Meat

Baş, Salih Zeki; Gülce, Handan; Yıldız, Salih

doi:10.1080/00914037.2013.854215

Cited by 8 publications

(1 citation statement)

References 47 publications

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“…Baş et al developed a biosensor based on immobilization of XO on a Pt electrode. This method could successfully detect hypoxanthine in fish samples, which can be used for the evaluation of fish freshness [14]. Sadeghi et al [15] have immobilized AO on Fe 3 O 4 -graphene oxide/poly-vinylpyrrolidone/polyaniline Nanocomposite in order to construct an amperometric biosensor for the determination of benzaldehyde.…”

Section: Introductionmentioning

confidence: 99%

Aldehyde oxidase assay by capillary electrophoresis: From off‐line, online up to immobilized enzyme reactor

Huang

Celá

Adams

et al. 2020

J of Separation Science

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Capillary electrophoresis is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of enzyme kinetics during drug development. Homogeneous or heterogeneous approaches can be followed for the enzymatic incubation. In this study, an immobilization procedure of aldehyde oxidase on magnetic particles was developed considering their integration with capillary electrophoresis. A number of magnetic nano/microparticle types were tested for this purpose, showing that aldehyde oxidase was most active when immobilized on bare silica magnetic nanoparticles. Primarily, the reusability of the enzyme immobilized on bare silica nanoparticles was tested. Three consecutive incubations with substrate could be performed, but the activity considerably dropped after the first incubation. One reason could be an enzyme detachment from the nanoparticles, but no release was detected neither at 4°C nor at 37°C during 5 h. The drop in enzymatic activity observed in consecutive incubations, could also be due to inactivation of the enzyme over time at given temperature. For the immobilized enzyme stored at 4°C, the activity decreased to 83% after 5 h, in contrast with a steep decrease at 37°C to 37%.

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Section: Introductionmentioning

confidence: 99%