Construction of biosensor for hypoxanthine determination by immobilization of xanthine oxidase and uricase in polypyrrole-paratoluenesulfonate film

Erol, Ezgi; Yıldırım, Ertan; Çete, Servet

doi:10.1007/s10008-020-04715-x

Cited by 20 publications

(7 citation statements)

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“…Previous studies of the effect of pH on the activity of PaoABC, another molybdenum hydroxylase, also highlight the critical role of the electrostatic interactions between the protein and the redox mediator [48] . Moreover, previous studies on XOD amperometric biosensors also recommend working under basic pH [24,49] . In our case, a pH of 8.3 was selected for subsequent experiments.…”

Section: Resultsmentioning

confidence: 92%

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Wiring Xanthine Oxidase Using an Osmium‐Complex‐Modified Polymer for Application in Biosensing.

et al. 2022

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Dedicated to Prof. Dr. Wolfgang Schuhmann on the occasion his 65 th birthdayXanthine is a metabolite of interest as a medical and food freshness biomarker. We modified a screen-printed electrode to detect this analyte by co-entrapping xanthine oxidase (XOD) and an Os-complex redox polymer over carbon nanotubes. In nature, XOD transfers its electrons to oxygen in solution. We demonstrate that the introduction of the redox polymer allows routing the electrons efficiently to the electrode surface, even under air-saturated conditions, enabling superior catalytic current and sensing performance. The bioanode was optimized by adjusting the electrode materials and the ratio of enzyme to redox polymer. The impacts of pH and ionic strength of the electrolyte on the sensor performance were also studied. We found that these variables can affect the electrostatic interaction between the enzyme and the redox polymer, and therefore impact the catalytic current extracted from xanthine oxidation. The XOD-bioanode design was combined with a bienzymatic cathode operating on glucose to demonstrate a biofuel cell (BFC). The resulting device could generate a power output of 16.56 μW cm À 2 at 0.25 V and an open-circuit voltage (OCV) of 0.50 V using 500 μM xanthine as biofuel. The proposed xanthine/glucose BFC showed promising features for application as a self-powered xanthine biosensor.

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Section: Resultsmentioning

confidence: 92%

“…[48] Moreover, previous studies on XOD amperometric biosensors also recommend working under basic pH. [24,49] In our case, a pH of 8.3 was selected for subsequent experiments.…”

Section: Investigation Of the Effect Of Phmentioning

confidence: 99%

Wiring Xanthine Oxidase Using an Osmium‐Complex‐Modified Polymer for Application in Biosensing.

et al. 2022

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“…Oxidative stress can damage cell components and cause dysfunction by inducing reactive oxygen species (ROS) generation under abiotic and biotic stresses (Demidchik, 2015; Casartelli et al, 2019; Erol et al, 2020). The change in gene expression induced by oxidative stress aims to prevent the overproduction of ROS and improve resistance to abiotic and biotic stress (Demidchik, 2015; You & Chan, 2015).…”

Section: Functional Involvements Of Map3ks In Abiotic Stressesmentioning

confidence: 99%

Modulation of phosphorylation status of MAP3 kinases under abiotic stress responses

Jeong,

Lim,

Kim

et al. 2024

Physiologia Plantarum

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Plants must face various environmental stresses that they cannot avoid, and they have developed numerous mechanisms to survive these challenges. Phosphate‐mediated signal transduction is a common method for regulating gene expression and protein activity in response to stresses. Phosphate groups can be transferred to other proteins by proteins called kinases. MAP3Ks are the largest kinase family in plants, and they comprise various group and individual pathways collectively known as MAPK cascades. When plants encounter environmental stresses, several MAP3Ks act as positive or negative regulators by phosphorylating downstream proteins within the MAPK cascade, as well as transcription factors, cytoskeletal proteins, enzymes, and kinases, through their kinase activity. In addition, other upstream kinases and phosphatases regulate MAP3Ks by adding or removing phosphate groups. Various MAP3Ks have been isolated in many plant species, and evidence suggests that they function as post‐translational modulators under various abiotic stresses, including ABA responses as well as salinity, osmotic, drought, oxidative, cold, and heat stress. This review focuses on how plant MAP3Ks affect various abiotic stresses as regulators and substrates through post‐translational phosphorylation.

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“…The one-step immobilization technique has helped to produce functionalized films possessing an optimal working pH close to that of free enzymes to conserve their conformation and microenvironment. PPY, PEDOT: PSS, and polyaminobenzoic acid (PABA) are some common polymers used in biosensor fabrication [ 54 , 55 , 56 , 57 ]. A biosensor for 17β-estradiol detection is fabricated based on a benzothiadiazole derivative of EDOT [ 58 ].…”

Section: Retention Of Biorecognition Molecules By Conducting Polymmentioning

confidence: 99%

Recent Advances of Conducting Polymers and Their Composites for Electrochemical Biosensing Applications

Luong

Narayan

Solanki

et al. 2020

JFB

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Conducting polymers (CPs) have been at the center of research owing to their metal-like electrochemical properties and polymer-like dispersion nature. CPs and their composites serve as ideal functional materials for diversified biomedical applications like drug delivery, tissue engineering, and diagnostics. There have also been numerous biosensing platforms based on polyaniline (PANI), polypyrrole (PPY), polythiophene (PTP), and their composites. Based on their unique properties and extensive use in biosensing matrices, updated information on novel CPs and their role is appealing. This review focuses on the properties and performance of biosensing matrices based on CPs reported in the last three years. The salient features of CPs like PANI, PPY, PTP, and their composites with nanoparticles, carbon materials, etc. are outlined along with respective examples. A description of mediator conjugated biosensor designs and enzymeless CPs based glucose sensing has also been included. The future research trends with required improvements to improve the analytical performance of CP-biosensing devices have also been addressed.

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Construction of biosensor for hypoxanthine determination by immobilization of xanthine oxidase and uricase in polypyrrole-paratoluenesulfonate film

Cited by 20 publications

References 58 publications

Wiring Xanthine Oxidase Using an Osmium‐Complex‐Modified Polymer for Application in Biosensing.

Wiring Xanthine Oxidase Using an Osmium‐Complex‐Modified Polymer for Application in Biosensing.

Modulation of phosphorylation status of MAP3 kinases under abiotic stress responses

Recent Advances of Conducting Polymers and Their Composites for Electrochemical Biosensing Applications

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