Hypoviral‐regulated HSP90 co‐chaperone p23 (<i>CpCop23</i>) determines the colony morphology, virulence, and viral response of chestnut blight fungus<i>Cryphonectria parasitica</i>

Ko, Yo-Han; Chun, Jeesun; Yang, Han-Eul; Kim, Dae‐Hyuk

doi:10.1111/mpp.13308

Cited by 4 publications

(1 citation statement)

References 65 publications

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“…To investigate the function of the CaRab7 gene, CaRab7 null mutants were generated by vector construction and transformation as previously described ( 76 ). For genomic DNA (gDNA) isolation, the mycelia of virus-free strain YC-31-P23 were cultured at 28°C at 180 rpm for 5 days, and gDNA was extracted using the cetyltrimethylammonium bromide (CTAB) procedure.…”

Section: Methodsmentioning

confidence: 99%

A novel partitivirus conferring hypovirulence by affecting vesicle transport in the fungus Colletotrichum

Zhu,

Qiu,

Gao

et al. 2024

mBio

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Colletotrichum spp. are economically important phytopathogenic fungi that cause anthracnose in a variety of plant species worldwide. Hypovirulence-associated mycoviruses provide new options for the biological control of plant fungal diseases. Here, we found a novel partitivirus from Colletotrichum alienum and named it Colletotrichum alienum partitivirus 1 (CaPV1). CaPV1 contained two dsRNA segments encoding an RNA-dependent RNA polymerase and a capsid protein and was classified under the genus Gammapartitivirus of the family Partitiviridae . CaPV1 significantly decreased host virulence, mycelial growth, appressorial development, and appressorium turgor but increased conidial production with abnormal morphology. In addition, CaPV1 could be successfully transfected into other Colletotrichum species, including C. fructicola , C. spaethianum , and C. gloeosporioides, and caused hypovirulence, indicating the broad application potential of this virus. CaPV1 caused significant transcriptional rewiring of the host fungus C. alienum . Notably, some genes related to vesicle transport in the CaPV1-infected strain were downregulated, consistent with the impaired endocytosis pathway in this fungus. When the Rab gene CaRab7, which is associated with endocytosis in vesicle transport, was knocked out, the virulence of the mutants was reduced. Overall, our findings demonstrated that CaPV1 has the potential to control anthracnose caused by Colletotrichum , and the mechanism by which Colletotrichum induces hypovirulence is caused by affecting vesicle transport. IMPORTANCE Colletotrichum is a kind of economically important phytopathogenic fungi that cause anthracnose disease in a variety of plant species worldwide. We found a novel mycovirus of the Gammapartitiviru s genus and Partitiviridae family from the phytopathogenic fungus Colletotrichum alienum and named it CaPV1. This study revealed that CaPV1 infection significantly decreased host virulence and fitness by affecting mycelial growth, appressorial development, and appressorium turgor. In addition, CaPV1 could also infect other Colletotrichum species, including C. fructicola , C. spaethianum , and C. gloeosporioides , by viral particle transfection and resulting in hypovirulence of these Colletotrichum species. Transcriptomic analysis showed that CaPV1 caused significant transcriptional rewiring of the host fungus C. alienum , especially the genes involved in vesicle transport. Moreover, endocytosis and gene knockout assays demonstrated that the mechanism underlying CaPV1-induced hypovirulence is, at least in part, caused by affecting the vesicle transport of the host fungus. This study provided insights into the mechanisms underlying the pathogenesis of Colletotrichum species and mycovirus-fungus interactions, linking the role of mycovirus and fungus vesicle transport systems in shaping fungal pathogenicity.

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Section: Methodsmentioning

confidence: 99%

A novel partitivirus conferring hypovirulence by affecting vesicle transport in the fungus Colletotrichum

Zhu,

Qiu,

Gao

et al. 2024

mBio

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Hypoviral‐regulated HSP90 co‐chaperone p23 (CpCop23) determines the colony morphology, virulence, and viral response of chestnut blight fungusCryphonectria parasitica

Chun

Yang

et al. 2023

Molecular Plant Pathology

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We previously identified a protein spot that showed down‐regulation in the presence of Cryphonectria hypovirus 1 (CHV1) and tannic acid supplementation as a Hsp90 co‐chaperone p23 gene ( CpCop23 ). The CpCop23 ‐null mutant strain showed retarded growth with less aerial mycelia and intense pigmentation. Conidia of the CpCop23 ‐null mutant were significantly decreased and their viability was dramatically diminished. The CpCop23 ‐null mutant showed hypersensitivity to Hsp90 inhibitors. However, no differences in responsiveness were observed after exposure to other stressors such as temperature, reactive oxygen species, and high osmosis, the exception being cell wall‐disturbing agents. A severe reduction in virulence was observed in the CpCop23 ‐null mutant. Interestingly, viral transfer to the CpCop23 ‐null mutant from CHV1‐infected strain via anastomosis was more inefficient than a comparable transfer with the wild type as a result of decreased hyphal branching of the CpCop23 ‐null mutant around the peripheral region, which resulted in less fusion of the hyphae. The CHV1‐infected CpCop23 ‐null mutant exhibited recovered mycelial growth with less pigmentation and sporulation. The CHV1‐transfected CpCop23 ‐null mutant demonstrated almost no virulence, that is, even less than that of the CHV1‐infected wild type (UEP1), a further indication that reduced virulence of the mutant is not attributable exclusively to the retarded growth but rather is a function of the CpCop23 gene. Thus, this study indicates that CpCop23 plays a role in ensuring appropriate mycelial growth and development, spore viability, responses to antifungal drugs, and fungal virulence. Moreover, the CpCop23 gene acts as a host factor that affects CHV1‐infected fungal growth and maintains viral symptom development.

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Changes in VOCs from a chestnut blight fungus Cryphonectria parasitica by a hypovirus infection: mycoviral infection alters fungal smell for insect vectors

Ko,

Chun,

Lee

et al. 2024

Chem. Biol. Technol. Agric.

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Hypoviral‐regulated HSP90 co‐chaperone p23 (CpCop23) determines the colony morphology, virulence, and viral response of chestnut blight fungusCryphonectria parasitica

Cited by 4 publications

References 65 publications

A novel partitivirus conferring hypovirulence by affecting vesicle transport in the fungus Colletotrichum

A novel partitivirus conferring hypovirulence by affecting vesicle transport in the fungus Colletotrichum

Hypoviral‐regulated HSP90 co‐chaperone p23 (CpCop23) determines the colony morphology, virulence, and viral response of chestnut blight fungusCryphonectria parasitica

Changes in VOCs from a chestnut blight fungus Cryphonectria parasitica by a hypovirus infection: mycoviral infection alters fungal smell for insect vectors

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