Hypouricemic and nephroprotective effects of palmatine from Cortex Phellodendri Amurensis: A uric acid modulator targeting Keap1-Nrf2/NLRP3 axis

Ai, Gaoxiang; Huang, Rong-Lei; Xie, Jianhui; Zhong, Linjiang; Wu, Xiaoyan; Qin, Zehui; Su, Zi‐Ren; Chen, Jiannan; Yang, Xiaobo; Dou, Yao-Xing

doi:10.1016/j.jep.2022.115775

Cited by 15 publications

(7 citation statements)

References 60 publications

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“…Atractylenolide I can alleviate hyperuricemia through AGE‐RAGE‐ and HIF‐1‐related pathways [22]. Palmatine improve hyperuricemia by regulating the expression of urate transport‐related protein URAT1 and inhibiting the expression of xanthine oxidase [23]. In addition, palmatine can upregulate the expression of NQO1, GCLC, GCLM, and HO‐1, downregulate the expression of Keap1, and promote Nrf2 translocation to reduce uric acid and protecting kidney [23].…”

Section: Resultsmentioning

confidence: 99%

“…Palmatine improve hyperuricemia by regulating the expression of urate transport‐related protein URAT1 and inhibiting the expression of xanthine oxidase [23]. In addition, palmatine can upregulate the expression of NQO1, GCLC, GCLM, and HO‐1, downregulate the expression of Keap1, and promote Nrf2 translocation to reduce uric acid and protecting kidney [23]. Atractylenolide II and atractylenolide III upregulate the expression of PPAR‐α and downregulate the expression of PPAR‐γ and Glut4 by activating AMPK signaling pathway for lowering blood sugar and blood lipid levels [24, 25].…”

Section: Resultsmentioning

confidence: 99%

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Chemical profiling of Simiao pill and quantification of main effective constituents in it by ultra‐high‐performance liquid chromatography coupled with Q Exactive Orbitrap and triple quadrupole mass spectrometry

Yang,

Sun,

Wei

et al. 2024

J of Separation Science

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Simiao pill is one of the most commonly used prescriptions in traditional Chinese medicine for the treatment of hyperuricemia and gout. However, methods based on more accurate and comprehensive qualitative and quantitative analyses of the active ingredients are not yet perfect due to limited methodology. This not only hinders the elucidation of the pharmacological mechanism of Simiao pill, but also its comprehensive clinical development and utilization. In this study, we employed ultra‐high‐performance liquid chromatography‐Q Exactive Orbitrap‐mass spectrometry technology to perform rapid analysis and identification of the chemical constituents in Simiao pill. A total of 101 chemical components were identified, including 26 alkaloids, 15 terpenoids, 11 flavonoids, eight steroids, six fatty acids, five limonoids, four saponins, five phenylpropanoids, and 21 other compounds. In addition, we established a new method by high‐throughput ultra‐high‐performance liquid chromatography‐Q Exactive Orbitrap‐mass spectrometry combined with ultra‐high‐performance liquid chromatography‐triple quadrupole‐tandem mass spectrometry technology for quantification of 14 main active ingredients, such as adenosine (1), phellodendrine (2), mangnoflorine (3), β‐ecdysterone (4), 25R‐inokosterone (5), 25S‐inokosterone (6), jatrorrhizine (7), palmatine (8), chikusetsu saponin IVa (9), limonin (10), atractylenolide III (11), atractylenolide I (12), obacunone (13), and atractylenolide II (14) in Simiao pill. This work laid a foundation for further analysis and quality control of effective components in Simiao pill.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Chemical profiling of Simiao pill and quantification of main effective constituents in it by ultra‐high‐performance liquid chromatography coupled with Q Exactive Orbitrap and triple quadrupole mass spectrometry

Yang,

Sun,

Wei

et al. 2024

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…It has various pharmacological properties, such as antibacterial and anti-inflammatory activities, liver and kidney protection, and uric acid-lowering effects. [ 59 ] Researchers have found that PAL decreases the mRNA expression and levels of pro-inflammatory cytokines in a dose-dependent manner. Superoxide dismutase (SOD) and glutathione (GSH) levels were remarkably enhanced, while malondialdehyde levels were reduced.…”

Section: Resultsmentioning

confidence: 99%

Exploring effect of herbal monomers in treating gouty arthritis based on nuclear factor-kappa B signaling: A review

Guo,

Ye,

Tang

et al. 2024

Medicine

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Gouty arthritis (GA) is an inflammatory disease caused by disorders of the purine metabolism. Although increasing number of drugs have been used to treat GA with the deepening of relevant research, GA still cannot be cured by simple drug therapy. The nuclear factor-kappa B (NF-κB) signaling pathway plays a key role in the pathogenesis of GA. A considerable number of Chinese herbal medicines have emerged as new drugs for the treatment of GA. This article collected relevant research on traditional Chinese medicine monomers in the treatment of GA using NF-κB, GA, etc. as keywords; and conducted a systematic search of relevant published articles using the PubMed database. In this study, we analyzed the therapeutic effects of traditional Chinese medicine monomers on GA in the existing literature through in vivo and in vitro experiments using animal and cell models. Based on this review, we believe that traditional Chinese medicine monomers that can treat GA through the NF-κB signaling pathway are potential new drug development targets. This study provides research ideas for the development and application of new drugs for GA.

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“…The increasing requirements for quality control of TCMs have resulted in the gradual replacement of the traditional single-compound measurement method with multi-component measurement, which has become a prominent approach in evaluating quality [21,22]. Various chemical compounds have been extracted from PAC, mainly concentrated on alkaloids that are considered to be the primary bioactive constituents contributing to its overall pharmacological effects [23,24] The diverse configurations of alkaloids exhibit varying biological activities in order to achieve therapeutic effects. The selected index ingredients in this study were chosen to be highly correlated with clinical efficacy to the greatest extent possible.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the key ingredient from the main production areas of Phellodendri Amurensis Cortex using ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry and ultra‐high‐performance liquid chromatography coupled to triple‐quadrupole mass spectrometry

Sun,

Zhao,

Fang

et al. 2024

J of Separation Science

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Phellodendri Amurensis Cortex (PAC) is a medicinal herb that has been generally used to treat diarrhea and jaundice. In order to comprehensively evaluate the PAC in the main production areas quality, a qualitative and quantitative method with highly effective, sensitive, and reliable was developed. The chemical compositions of PAC were analyzed, and fingerprints were established by ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐Q‐TOF‐MS). Then, the determination of berberine, canthin‐6‐one, dictamnine, γ‐fagarine, and magnoflorine from PAC samples was simultaneously performed using UPLC‐QQQ‐MS. Furthermore, the chemical components of PAC from different regions were compared and analyzed by combining hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. A total of 58 compounds were identified, including 36 alkaloids, four phenylpropanoids, seven terpenoids, four flavonoids and their glycosides, an organic acid compound, and six other components. The fingerprint results show that samples have good similarity. Meanwhile, the content of the five ingredients in different habitats is quite different. By multivariate statistical analysis, 18 batches of PAC could be divided into three categories, and 20 components were identified as differential markers of various origins. A comprehensive method of PAC quality evaluation and chemical composition difference analysis was established, which provided the scientific basis for quality evaluation and further pharmacological mechanism research.

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Hypouricemic and nephroprotective effects of palmatine from Cortex Phellodendri Amurensis: A uric acid modulator targeting Keap1-Nrf2/NLRP3 axis

Cited by 15 publications

References 60 publications

Chemical profiling of Simiao pill and quantification of main effective constituents in it by ultra‐high‐performance liquid chromatography coupled with Q Exactive Orbitrap and triple quadrupole mass spectrometry

Chemical profiling of Simiao pill and quantification of main effective constituents in it by ultra‐high‐performance liquid chromatography coupled with Q Exactive Orbitrap and triple quadrupole mass spectrometry

Exploring effect of herbal monomers in treating gouty arthritis based on nuclear factor-kappa B signaling: A review

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