Evaluation of the key ingredient from the main production areas of Phellodendri Amurensis Cortex using ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry and ultra‐high‐performance liquid chromatography coupled to triple‐quadrupole mass spectrometry

Abstract

Phellodendri Amurensis Cortex (PAC) is a medicinal herb that has been generally used to treat diarrhea and jaundice. In order to comprehensively evaluate the PAC in the main production areas quality, a qualitative and quantitative method with highly effective, sensitive, and reliable was developed. The chemical compositions of PAC were analyzed, and fingerprints were established by ultra‐high‐performance liquid chromatography‐quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐Q‐TOF‐MS). Then, the determination of berberine, canthin‐6‐one, dictamnine, γ‐fagarine, and magnoflorine from PAC samples was simultaneously performed using UPLC‐QQQ‐MS. Furthermore, the chemical components of PAC from different regions were compared and analyzed by combining hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. A total of 58 compounds were identified, including 36 alkaloids, four phenylpropanoids, seven terpenoids, four flavonoids and their glycosides, an organic acid compound, and six other components. The fingerprint results show that samples have good similarity. Meanwhile, the content of the five ingredients in different habitats is quite different. By multivariate statistical analysis, 18 batches of PAC could be divided into three categories, and 20 components were identified as differential markers of various origins. A comprehensive method of PAC quality evaluation and chemical composition difference analysis was established, which provided the scientific basis for quality evaluation and further pharmacological mechanism research.

Integration of High-Resolution LC-Q-TOF Mass Spectrometry and Multidimensional Chemical−Biological Analysis to Detect Nanomolar-Level Acetylcholinesterase Inhibitors from Different Parts of Zanthoxylum nitidum

“…When RDA fragmentation occurred, the compound belonged to protoquinoline-type alkaloids . The characteristics of berberine-type alkaloids and furanquinoline-type alkaloids were the loss of CH 3 (15 Da) and C 2 H 6 (30 Da), respectively . When (CH 3 ) 2 NH (45 Da) was lost, the compound belonged to benzyltetrahydroisoquinoline-type alkaloids or aporphine-type alkaloids .…”

Analytical strategies to identify multicomponent quality markers for commercial Hua‐ju‐hong using multidimensional chemical analysis

“…Multi-component measurement has become a prominent approach for quality control of CMM and has resulted in the gradual replacement of the traditional single-compound measurement method [42,43]. However, naringin is still the main single compound for quality control of HJH nowadays.…”