Hypoglycemic and Hypolipidemic Effects of Phellinus Linteus Mycelial Extract from Solid-State Culture in A Rat Model of Type 2 Diabetes

Abstract

Hypoglycemic and hypolipidemic effects of P. linteus have been observed in numerous studies, but the underlying molecular mechanisms are unclear. In this study, we prepared P. linteus extract (PLE) from mycelia of solid-state culture, and evaluated its hypoglycemic and hypolipidemic effects in rat models of high-fat diet (HFD)-induced and low-dose streptozotocin (STZ)-induced type 2 diabetes. PLE treatment effectively reduced blood glucose levels, and improved insulin resistance and lipid and lipoprotein profiles. The hypoglycemic effect of PLE was based on inhibition of key hepatic gluconeogenesis enzymes (FBPase, G6Pase) expression and hepatic glycogen degradation, and consequent reduction of hepatic glucose production. PLE also: (i) enhanced expression of CPT1A and ACOX1 (key proteins involved in fatty acid β-oxidation) and low-density lipoprotein receptor (LDLR) in liver, thus promoting clearance of triglycerides and LDL-C; (ii) inhibited expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) in liver, thus reducing cholesterol production; (iii) displayed strong hepatoprotective and renal protective effects. Our findings indicate that PLE has strong potential functional food application in adjuvant treatment of type 2 diabetes with dyslipidemia.

Phellinus linteus polysaccharide extract improves insulin resistance by regulating gut microbiota composition

“…24 Mycelia were obtained by solidstate fermentation under uniform conditions, dried, and crushed into powder. 24 Mycelia were obtained by solidstate fermentation under uniform conditions, dried, and crushed into powder.…”

“…24 Six rats were selected randomly as the normal group (NC). All experimental procedures were approved by the Animal Care and Use Committee of Huazhong Agricultural University (protocol # SCXK2017-0061), and performed in accordance with internationally accepted guidelines and ethical principles.…”

“…24 Detailed information for Quantitative real-time PCR (qRT-PCR) primers of claudin 1 (Cldn1), zonula occludens 1 (ZO1), occludin (Ocln), and β-actin is shown in Table 1. Reverse transcription and PCR amplification were performed as described previously.…”

“…[19][20][21] The epithelial barrier blocks entry of pro-inflammatory factors such as LPS into the bloodstream and helps reduce chronic inflammatory response. 23,24 PLPE is rich in polysaccharides, which produce beneficial physiological effects including anti-tumor, 25 anti-inflammation, 26 and hepatoprotective. 23,24 PLPE is rich in polysaccharides, which produce beneficial physiological effects including anti-tumor, 25 anti-inflammation, 26 and hepatoprotective.…”

Optimized production and safety evaluation of hispidin‐enriched Sanghuangporus sanghuang mycelia

“…Phellinus linteus , also known as “sanghuang” in China, “meshimakobu” in Japan, and “sangwhang” in Korea, is a well‐known fungus and has been recognized as beneficial to health for more than 2,000 years (Chen, Tian, Miao, & Zhao, ). Numerous studies have demonstrated that P. linteus possesses antitumor, anti‐inflammatory, anti‐allergic, anti‐angiogenic, antioxidant, hypoglycemic, hypolipidemic, and immunomodulatory activities (Liu, Wang, Li, Mei, & Liang, ; Sliva, ). The pharmacological properties of P. linteus are often associated with the presence of high‑molecular‑weight and low‑molecular‑weight compounds.…”