Hypoglycemic activity of Nymphaea stellata leaves ethanolic extract in alloxan induced diabetic rats

Dhanabal, S. P.; Raja, M. K. Mohan Maruga; Ramanathan, Meena; Suresh, B.

doi:10.1016/j.fitote.2007.02.009

Cited by 45 publications

(30 citation statements)

References 5 publications

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“…The pancreas is especially susceptible to the action of alloxan induced free radical-damage; subsequently, there is a decrease in endogenous insulin and this affects the utilization of glucose by the body tissues. It also leads to elevated blood glucose level, decreased protein content and increased levels of serum cholesterol, triglycerides and other bad lipids [24,25]. The rats were considered diabetic whenever their FBS was ≥ 7.0 mMol/l and more [18,26].…”

Section: Discussionmentioning

confidence: 99%

Bioassay-Guided Isolation and Structural Elucidation of Antidiabetic Principle of Methanol Leaf Extract of Newbouldia laevis (P.Beauv)

Bosha¹,

Aondohulugh²,

Ottah³

et al. 2015

JPP

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NLE (Newbouldia laevis (P.Beauv)) is used in folk medicine to treat diabetes. The aim of the study was to isolate and characterize the active compound responsible for the antidiabetic activity.This was carried out using standard in vivo and in vitro models in rats. The antidiabetic activities were evaluated using alloxan-induced diabetes in male and female albino rats after an over night fast and various doses of NLE and glibenclamide, the reference drug 2.0 mg/kg. Bioassay-guided isolation/fractionation techniques were used to isolate the active compound. Characterization of the active compound was carried out including molecular and structural elucidation using NMR (nuclear magnetic resonance) and Gas-Chromatography Mass spectroscopy. The extract caused 60.2% reduction in the FBS (fasting blood sugar) of diabetic rats. Bioassay-guided fractionation of NLE yielded ten (10) fractions with F9 (fraction nine) as the active fraction, which caused 66.0% reduction of FBS in alloxan-induced diabetic rats. Further purification using preparative TLC (thin layer chromatography), gave sub-fraction 9.2 as the active compound. Sub-fraction 9.2 reduced the FBS by 61.4%. The characterization of F9:2 using nuclear magnetic resonance and MS (mass spectroscopy) confirmed it to be a polyunsaturated fatty acid with a molecular weight of 358.56 g.

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Section: Discussionmentioning

confidence: 99%

Bioassay-Guided Isolation and Structural Elucidation of Antidiabetic Principle of Methanol Leaf Extract of Newbouldia laevis (P.Beauv)

Bosha¹,

Aondohulugh²,

Ottah³

et al. 2015

JPP

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show abstract

“…Alloxan, a beta cytotoxin, destroys beta-cells of islets in the langerhans of pancreas resulting in a decreased endogenous insulin secretion leading to a decreased utilization of glucose by body tissues [19] . It results in the elevation of blood glucose level, decreases protein content, and increases levels of cholesterol and triglycerides [20] .…”

Section: Discussionmentioning

confidence: 99%

Histological changes and antidiabetic activities of Icacina trichantha tuber extract in beta–cells of alloxan induced diabetic rats

Monday

Uzoma

2013

Asian Pacific Journal of Tropical Biomedicine

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“…Thus, L. micranthus significe it`s antidiabetic and antihyperlipidemic property and strengthen the presence of various triterpenoids, flavonoids and phenolic compounds. Alloxan is a hydrophilic and unstable analogue of glucose and selectively destroys beta cells of islets of pancreas, results in the elevation of blood glucose level, decreases protein content, and increases levels of cholesterol and triglycerides [36]. Alloxan and its product dialuric acid establish a redox potential and forms superoxide radicals.…”

Section: Discussionmentioning

confidence: 99%

Gc-Ms Study of Two Column Fractions from Methanol Extracts of Loranthus Micranthus and Their In Vivo Antidiabetic Activity on Alloxan Induced Diabetic Rats

M¹

2015

J Diabetes Metab

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The methanol extracts of Loranthus micranthus fractions were collected from column chromatography. From TLC, the fraction 17 and 20 were selected based on the clear distiguishable of bands and few phytochemicals. Same fractions when subjected to GC-MS, fraction-17 yielded 13 and fraction-20 yielded 11 prominent compounds. Diabetic rats that were treated with the fraction 17 and 20 showed significantly increase in body weight (16.52 and 12.43 % respectively). Hypoglycemic effect and antihyperlipidemic characters (decrease in the previously elevated levels of serum cholesterol and serum triglycerides) were observed. The two fractions treated rats have also showed the regeneration of pancreatic connective tissue, acini, blood vessels, blood capillaries, pancreatic ducts and their walls, islets capsule and cells in accordance to proper cytoarchitecture. The in vivo antidiabetic property may be due to the presence of single or all phytochemicals in the fraction. Greater activity was observed in fraction-17. However, further experimental studies are still needed for identification of the functionally relevant phytochemical(s) in management of diabetes in rats.sp. were the common host plants of L. micranthus [10]. Herbals are the choice over synthetic drugs from past centuries having multiple therapeutic functions and fewer side effects. In this context it was an effort to attend towards antidibetic studies of L. micranthus in an analytical and systematic ways. L. micranthus has been widely used in ethnomedicine for various purposes, including antihypertensive, anticancer, antispasmodic, and antidiabetic, in treatment of epilepsy, headache, infertility, menopausal syndrome and rheumatism [11,12] and many of medicinal activities are proven by recent researches. It decreases the blood glucose level and controls the loss of body weight in diabetes mellitus [13].Presently the study was aimed to carry out GS-MS and in vivo antidiabetic activities of Loranthus micranthus on alloxan induced diabetic rats. Based on our earlier studies of qualitative, quantitative and some biological activities, column fractions from methanol extracts of L. micranthus were selected for in vivo antidiabetic studies on rats. Body weight, blood glucose level, lipid profile and histopathological changes of rats were considered in the study. Materials and Methods Collection and processing of plantThe fresh leaves of Loranthus micranthus growing on the host plant

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Hypoglycemic activity of Nymphaea stellata leaves ethanolic extract in alloxan induced diabetic rats

Cited by 45 publications

References 5 publications

Bioassay-Guided Isolation and Structural Elucidation of Antidiabetic Principle of Methanol Leaf Extract of Newbouldia laevis (P.Beauv)

Bioassay-Guided Isolation and Structural Elucidation of Antidiabetic Principle of Methanol Leaf Extract of Newbouldia laevis (P.Beauv)

Histological changes and antidiabetic activities of Icacina trichantha tuber extract in beta–cells of alloxan induced diabetic rats

Gc-Ms Study of Two Column Fractions from Methanol Extracts of Loranthus Micranthus and Their In Vivo Antidiabetic Activity on Alloxan Induced Diabetic Rats

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