Hypocholesterolemic Action of Lactobacillus acidophilus ATCC 43121 and Calcium in Swine with Hypercholesterolemia Induced by Diet

Abstract

Thirty-three Yorkshire barrows (92 kg), fed a high cholesterol diet for 14 d had mean concentrations of serum cholesterol of 294.6 +/- 7.8 mg/dl. Starting on d 15 and for an additional 15 d, crystalline cholesterol was removed from the diet and pigs were assigned to one of four treatments: including two levels of calcium (0.7% and 1.4%) with and without added viable Lactobacillus acidophilus ATCC 43121 (2.5 x 10(11) cells per feeding). Serum cholesterol levels decreased, as expected, for all groups. However, the declines were initiated sooner for the groups receiving L. acidophilus. and those receiving the high level of calcium than for the respective control groups. When averaged over days, pigs fed L. acidophilus had 11.8% lower total cholesterol than pigs fed a diet without L. acidophilus. Similarly, pigs fed 1.4% calcium had a significantly lower total cholesterol than pigs fed 0.7% calcium. The effects were greater on low density lipoprotein cholesterol than on high density lipoprotein cholesterol. In addition, during the overall 15-d experimental period, serum bile acids were reduced 23.9% by dietary L. acidophilus and by 21.4% by 1.4% dietary calcium compared with those of their controls. Total bile acid concentration was positively correlated with total cholesterol concentration for pigs fed L. acidophilus or 1.4% calcium. These data suggest that both L. acidophilus and calcium can enhance the reduction of serum cholesterol in pigs that had been fed a high cholesterol diet, probably through alteration in the enterohepatic circulation of bile acids.

Effects of Green Tea Extract and Lactobacillus Casei Strain Shirota on Levels of Serum Minerals, Cholesterol, Triglycerides, Glucose and Lactate in Rats Fed on High-carbohydrate and High-lipid Diets

“…Our study indicated that the rats fed on a high-carbohydrate diet and supplemented with L. casei strain Shirota had a decrease in their serum glucose levels. However, the rats fed on a highlipid diet did not show changes in serum cholesterol or triglyceride levels 38 . Wang et al suggested that L. plantarum MA2 decreased serum levels of total cholesterol, LDL cholesterol and triglyceride levels in rats fed on high-cholesterol diets 39 .…”

Yoghurt enriched with Lactobacillus acidophilus does not lower blood lipids in healthy men and women with normal to borderline high serum cholesterol levels

“…Lactobacillus acidophilus is the best studied of the`probiotic' cholesterol-lowering bacteria (Lin et al, 1989;Schaafsma et al, 1998;Harrison & Peat, 1975;Khedkar et al, 1993;de Rodas et al, 1996). Of all the Lactobacillus acidophilus-strains the L-1 strain, used in commercial products in the Netherlands, is thought to be one of the most effective strains to lower serum cholesterol (Buck & Gilliland, 1994).…”

Production of New Synbiotic Yoghurt with Local Probiotic Isolate and Oat and Study its Effect on Mice

“…The bile acid and cholesterol level in feces of the mice fed with yoghurt and probiotic bacteria (groups C & D) were higher than those of group B with highest concentration in group C. As deconjugated bile salts are more readily excreted in the feces than conjugated bile salts [37][38][39], bacteria with BSH activity may effectively reduce serum cholesterol by enhancing the excretion of bile salts, with a consequent increase in the synthesis of bile salts from serum cholesterol; or by decreasing the solubility of cholesterol, since bile acid is essential for gastrointestinal absorption of cholesterol [6] and thus reducing its uptake from the gut.…”