Hypocholesterolemia is associated negatively with hemolysate lipid peroxidation in sickle cell anemia patients

Abstract

The oxidative stress levels in plasma and hemolysate and cholesterol levels in plasma of sickle cell anemia patients, carriers and controls were evaluated. A total of 40 cases-17 patients, 13 carriers and 10 controls-were involved in the study. Plasma and hemolysate malondialdehyde (MDA) were detected via thiobarbituric acid reaction with a fluorimetric detector by high-performance liquid chromatography system. Plasma cholesterol was determined by enzymatic colorimetric method. Mean MDA levels of SCA patients were higher than those of the carriers' and healthy children's both in plasma and in hemolysate (P < 0.005). The mean plasma and hemolysate MDA levels were 25.3 ± 1.6 nmol/l and 86.7 ± 19.3 nmol/l in patients, 19.1 ± 0.8 nmol/l and 54.1 ± 10.8 nmol/l in carriers and 19.6 ± 0.8 nmol/l and 56.8 ± 9.3 nmol/l in healthy children. Mean plasma total cholesterol levels were 92.1 ± 19.1 mg/dl in patients, 116.2 ± 23.3 mg/dl in carriers and 126.6 ± 16.4 mg/dl in controls (P < 0.005). There was a significant negative correlation of -0.520 between hemolysate MDA and plasma cholesterol levels in patients (P < 0.05). The degree of correlation increased up to -0.782 (P = 0.008) in the patients with HbSS phenotype. This negative correlation between MDA and cholesterol may imply a potential association between oxidative stress and hypocholesterolemia in sickle cell anemia.

Hypocholesterolemia in Sickle Cell Anemia: What is the Reason behind it?

“…Oxidative stress can be another reason for hypocholesterolemia in SCA. In a recent study of our group a significant negative correlation was found between plasma cholesterol and hemolysate MDA levels of steady state SCA patients [6]. This data may indicate a decrease of either erythrocyte or plasma lipoprotein cholesterol perhaps due to a hypermetabolic state in response to oxidative stress.…”

Normal Non-HDL Cholesterol, Low Total Cholesterol, and HDL Cholesterol Levels in Sickle Cell Disease Patients in the Steady State: A Case-Control Study of Tema Metropolis

“…SCD is characterized by defect in plasma and erythrocyte lipids associated with chronic oxidative stress [6]. These two morbid processes disturb lipid homeostasis which in turn may lead to atherosclerosis in these patients [7, 8].…”

Structural Modification of Plasma Albumin in Sickle Cell Anemia

“…Besides the manifestations of chronic hemolysis, vaso-occlusion, endothelial injury and inflammation [1], SCA is also a disease of increased oxidative stress [2]. Previous studies have reported increased levels of malondialdehyde [2] and protein carbonyl [3] in the plasma of SCA patients.…”