Hypertonic Saline Resuscitation Restores Hemorrhage-Induced Immunosuppression by Decreasing Prostaglandin E2and Interleukin-4 Production

Coimbra, Raúl; Junger, Wolfgang G.; Hoyt, David B.; Liu, Forrest C.; Loomis, William; Evers, Michael F.

doi:10.1006/jsre.1996.0329

Cited by 99 publications

(53 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the other hand, when hypertonic saline was added to the culture media containing PGE 2 , there was a complete reversal on the PGE 2 -induced immunosupression. 47,48 These in vitro findings were confirmed in vivo using a hemorrhagic shock model in mice, in which the infusion of 7.5% NaCl reversed the cellular immune function depression that occurs after hemorrhage. Plasma levels of several cytokines were determined, and it was concluded that hyper-tonic saline solution prevents immunossupression probably by decreasing plasma levels of IL-4 and PGE 2 .…”

Section: Immune and Inflammatory Effects Of Hs/ Hsdmentioning

confidence: 87%

“…45 More recently, a series of experiments have shown that hypertonic saline solution, even without dextran, significantly interferes with the immune responses, both in vitro and in vivo. [46][47][48][49][50][51][52][53][54][55][56][57] The addition of hypertonic saline solution to a lymphocyte culture media, in concentrations similar to those obtained with systemic infusion of 4 mL/kg of 7.5% NaCl in humans, determined a significant increase in lymphocyte proliferation. 46 The addition of prostaglandin E 2 (PGE 2 ) to the culture media, in the absence of hypertonic saline, caused a reduction in T-cells proliferation, when compared to control cultures.…”

Section: Immune and Inflammatory Effects Of Hs/ Hsdmentioning

confidence: 99%

See 1 more Smart Citation

Small Volume Hypertonic Resuscitation of Circulatory Shock

Rocha-e-Silva

Figueiredo

2005

Clinics

View full text Add to dashboard Cite

Small volume hypertonic resuscitation is a relatively new conceptual approach to shock therapy. It was originally based on the idea that a relatively large blood volume expansion could be obtained by administering a relatively small volume of fluid, taking advantage of osmosis. It was soon realized that the physiological vasodilator property of hypertonicity was a useful byproduct of small volume resuscitation in that it induced reperfusion of previously ischemic territories, even though such an effect encroached upon the malefic effects of the ischemia-reperfusion process. Subsequent research disclosed a number of previously unsuspected properties of hypertonic resuscitation, amongst them the correction of endothelial and red cell edema with significant consequences in terms of capillary blood flow. A whole set of actions of hypertonicity upon the immune system are being gradually uncovered, but the full implication of these observations with regard to the clinical scenario are still under study. Small volume resuscitation for shock is in current clinical use in some parts of the world, in spite of objections raised concerning its safety under conditions of uncontrolled bleeding. These objections stem mainly from experimental studies, but there are few signs that they may be of real clinical significance. This review attempts to cover the earlier and the more recent developments in this field.

show abstract

Section: Immune and Inflammatory Effects Of Hs/ Hsdmentioning

confidence: 87%

Section: Immune and Inflammatory Effects Of Hs/ Hsdmentioning

confidence: 99%

Small Volume Hypertonic Resuscitation of Circulatory Shock

Rocha-e-Silva

Figueiredo

2005

Clinics

View full text Add to dashboard Cite

show abstract

“…Studies based on different animal models suggest that HTS and HES could either directly or indirectly alter the expression of cytokines and activation of signaling pathways, including those above (Coimbra et al, 1996;Loomis et al, 2001;Ke et al, 2006;Wang et al, 2009;Chen et al, 2013;Esnault et al, 2013;Liu et al, 2013;Choi et al, 2014;Dong et al, 2014;Huang et al, 2014;Igarashi et al, 2014;Wright et al, 2014;Öztürk et al, 2015;Chang et al, 2016). Thus, the MDSC-related cytokines and signaling pathways in hemorrhagic shock resuscitation with different kinds of fluids need further research.…”

Section: Fig 3 Variations Of Mdscs and M-mdsc/g-mdsc Ratio In Mice Bmentioning

confidence: 99%

Distribution and differentiation of myeloid-derived suppressor cells after fluid resuscitation in mice with hemorrhagic shock

Jiang

Fang

Ling

et al. 2017

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:Objective: To investigate the distribution and differentiation of myeloid-derived suppressor cells (MDSCs) in hemorrhagic shock mice, which are resuscitated with normal saline (NS), hypertonic saline (HTS), and hydroxyethyl starch (HES). Methods: BALB/c mice were randomly divided into control, NS, HTS, and HES resuscitation groups. Three subgroups (n=8) in each resuscitation group were marked as 2, 24, and 72 h. Flow cytometry was used to detect the MDSCs, monocytic MDSCs (M-MDSCs), and granulocytic/neutrophilic MDSCs (G-MDSCs) in peripheral blood nucleated cells (PBNCs), spleen single-cell suspension, and bone marrow nucleated cells (BMNCs). Results: The MDSCs in BMNCs among three resuscitation groups were lower 2 h after shock, in PBNCs of the HTS group were higher, and in spleen of the NS group were lower (all P<0.05 vs. control). The M-MDSC/G-MDSC ratios in PBNCs of the HTS and HES groups were lower (both P<0.05 vs. control). At 24 h, the MDSCs in PBNCs of the NS and HTS groups were higher, while the spleen MDSCs in the HTS group were higher (all P<0.05 vs. control). The M-MDSC/ G-MDSC ratios were all less in PBNCs, spleen, and BMNCs of the NS and HTS groups, and were lower in BMNCs of the HES group (all P<0.05 vs. control). At 72 h, the elevated MDSCs in PBNCs were presented in the HTS and HES groups, and in spleen the augment turned up in three resuscitation groups (all P<0.05 vs. control). The inclined ratios to M-MDSC were exhibited in spleen of the NS and HTS groups, and in PBNCs of the NS group; the inclination to G-MDSC in BMNCs was shown in the HES group (all P<0.05 vs. control). Conclusions: HTS induces the earlier elevation of MDSCs in peripheral blood and spleen, and influences its distribution and differentiation, while HES has a less effect on the distribution but a stronger impact on the differentiation of MDSCs, especially in bone marrow.

show abstract

“…Рядом авторов предоставлена информация о том, что ги пертоничность при использовании ГР способна усиливать про лиферацию лимфоцитов и ингибировать функции нейтрофи лов в культуре клеток крови человека и различных моделях животных [46,47]. J. Kolsen Peterson и соавт.…”

Section: о б з о р ыunclassified