Hyperthermia with Magnetic Nanowires for Inactivating Living Cells

Ds, Choi; J, Park; S, Kim; Gracias, David H.; Mk, Cho; Yk, Kim; Fung, Andrew O.; Lee, Jun Young; Chen, Y; Khanal, Sohana; Baral, Saroj Sundar; Kim, Jae Hyun

doi:10.1166/jnn.2008.273

Cited by 61 publications

(48 citation statements)

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“…Further, studies on destruction of living cells have paved the way for possible cancer treatment therapies. For instance, it has been shown that cells with internalized Ni NWs can be heated and killed under a radio frequency electromagnetic field (Choi et al 2008). Similarly, an alternating current magnetic field has been proven to increase the inflammation of cells with internalized Ni NWs due to an increase in the pro-inflammatory cytokine interleukin-6 (Choi et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and intracellular dissolution of nickel nanowires

Perez

Contreras²,

Vilanova

et al. 2016

Nanotoxicology

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The assessment of cytotoxicity of nanostructures is a fundamental step for their development as biomedical tools. As widely used nanostructures, nickel nanowires (Ni NWs) seem promising candidates for such applications. In this work, Ni NWs were synthesized and then characterized using vibrating sample magnetometry, energy dispersive X-Ray analysis and electron microscopy. After exposure to the NWs, cytotoxicity was evaluated in terms of cell viability, cell membrane damage and induced apoptosis/necrosis on the model human cell line HCT 116. The influence of NW to cell ratio (10:1 to 1000:1) and exposure times up to 72 hours was analyzed for Ni NWs of 5.4 µm in length, as well as for Ni ions. The results show that cytotoxicity markedly increases past 24 hours of incubation. Cellular uptake of NWs takes place through the phagocytosis pathway, with a fraction of the dose of NWs dissolved inside the cells. Cell death results from a combination of apoptosis and necrosis, where the latter is the outcome of the secondary necrosis pathway. The cytotoxicity of Ni ions and Ni NWs dissolution studies suggest a synergistic toxicity between NW aspect ratio and dissolved Ni, with the cytotoxic effects markedly increasing after 24 hours of incubation.Just Accepted by Nanotoxicology © 2015 Taylor & Francis. This provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon.DISCLAIMER: The ideas and opinions expressed in the journal's Just Accepted articles do not necessarily reflect those of Taylor & Francis (the Publisher), the Editors or the journal. The Publisher does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of the material contained in these articles. The reader is advised to check the appropriate medical literature and the product information currently provided by the manufacturer of each drug to be administered to verify the dosages, the method and duration of administration, and contraindications. It is the responsibility of the treating physician or other health care professional, relying on his or her independent experience and knowledge of the patient, to determine drug dosages and the best treatment for the patient. Just Accepted articles have undergone full scientific review but none of the additional editorial preparation, such as copyediting, typesetting, and proofreading, as have articles published in the traditional manner. There may, therefore, be errors in Just Accepted articles that will be corrected in the final print and final online version of the article. Any use of the Just Accepted articles is subject to the express understanding that the papers have not yet gone through the full quality control process prior to publication. Cytotoxicity and intracellular dissolution of nickel nanowiresJose E. Perez 1, 2 , Maria F. Contreras 1 , Enrique Vilanova 2 , Laura P. Felix 1, 2 , Michael B.

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Section: Introductionmentioning

confidence: 99%

Cytotoxicity and intracellular dissolution of nickel nanowires

Perez

Contreras²,

Vilanova

et al. 2016

Nanotoxicology

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“…The most carcinogenic ni-compounds are those that do not induce apoptosis. 12 Ni-induced apoptosis was reported on Chinese hamster ovary (CHO) cells, T-cell hybridoma, Jurkat cells, and mouse epidermal JB-6 cells. 5 By definition, a nanowire (NW) is a nanostructure that has a diameter restricted to tens of nanometers (10 -9 m) or less and an unconstrained length which shows an aspect ratio (length-to-width) of $1000 higher.…”

Section: Introductionmentioning

confidence: 99%

Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells

Hossain¹,

Kleve²

2011

IJN

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Background The ability to evade apoptosis is one of the key properties of cancer. The apoptogenic effect of nickel nanowires (Ni NWs) on cancer cell lines has never been adequately addressed. Due to the unique physicochemical characteristics of Ni NWs, we envision the development of a novel anticancer therapeutics specifically for pancreatic cancer. Thus, we investigated whether Ni NWs induce ROS-mediated apoptosis in human pancreatic adenocarcinoma (Panc-1) cells. Methods In this study Ni NWs were fabricated using the electrodeposition method. Synthesized Ni NWs were physically characterized by energy dispersive X-ray analysis, UV-Vis spectroscopy of NanoDrop 2000 (UV-Vis), magnetization study, scanning electron microscopy, and transmission electron microscopy. Assessment of morphological apoptotic characteristics by phase contrast microscopy (PCM), Ni-NWs-induced apoptosis staining with ethidium bromide (EB) and acridine orange (AO) followed by fluorescence microscopy (FM) was performed. For molecular biological and biochemical characterization, Panc-1 cell culture and cytotoxic effect of Ni NWs were determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Quantitative apoptosis was analyzed by flow cytometry staining with propidium iodide through cell cycle arrest and generation of ROS using 2′, 7′-dichlorofluorescein diacetate fluorescence intensity. In all experiments, Panc-1 cancer cells without any treatment were used as the negative controls. Results The intracellular uptake of Ni NWs through endocytosis by Panc-1 cells was observed by PCM. EB and AO staining of FM and MTT assay qualitatively and quantitatively confirmed the extent of apoptosis. Flow cytometric cell cycle arrest and ROS generation indicated Ni NWs as inducers of apoptotic cell death. Conclusion We investigated the role of Ni NWs as inducers of ROS-mediated apoptosis in Panc-1 cells. These results suggested that Ni NWs could be an effective apoptotic agent for Panc-1 cells and have good potential for further research into a clinical treatment selective for pancreatic cancer.

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“…Furthermore, Ni NWs have proven to be efficient in cell separation, manipulation, and purification 16,17,21,22,[24][25][26] as well as in delivering cargoes of various macro-particles including biological entities. 27 Recent studies have investigated their use as therapeutic agents for hyperthermia 28 and cell inflammation induction 29 in cultures of human embryonic cells. They have also been shown to work as apoptotic agents for pancreatic cancer cells.…”

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confidence: 99%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

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In this paper, we show that magnetic nanowires with weak magnetic fields and low frequencies can induce cell death via a mechanism that does not involve heat production. We incubated colon cancer cells with two concentrations (2.4 and 12 μg/mL) of nickel nanowires that were 35 nm in diameter and exposed the cells and nanowires to an alternating magnetic field (0.5 mT and 1 Hz or 1 kHz) for 10 or 30 minutes. This low-power field exerted a force on the magnetic nanowires, causing a mechanical disturbance to the cells. Transmission electron microscopy images showed that the nanostructures were internalized into the cells within 1 hour of incubation. Cell viability studies showed that the magnetic field and the nanowires separately had minor deleterious effects on the cells; however, when combined, the magnetic field and nanowires caused the cell viability values to drop by up to 39%, depending on the strength of the magnetic field and the concentration of the nanowires. Cell membrane leakage experiments indicated membrane leakage of 20%, suggesting that cell death mechanisms induced by the nanowires and magnetic field involve some cell membrane rupture. Results suggest that magnetic nanowires can kill cancer cells. The proposed process requires simple and low-cost equipment with exposure to only very weak magnetic fields for short time periods.

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Hyperthermia with Magnetic Nanowires for Inactivating Living Cells

Cited by 61 publications

References 0 publications

Cytotoxicity and intracellular dissolution of nickel nanowires

Cytotoxicity and intracellular dissolution of nickel nanowires

Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells

Non-chemotoxic induction of cancer cell death using magnetic nanowires

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