Hyperspectral imaging-based exosome microarray for rapid molecular profiling of extracellular vesicles

Abstract

One of the challenges for exploiting extracellular vesicle (EV) as a disease biomarker is to differentiate EVs released by similar cell types or phenotypes. The paper reports a high-throughput and...

An integrated magneto-opto-fluidic biosensor for rapid on-chip assay of respiratory viruses of livestock

“…The PC biosensor measures the change of the refractive index induced by the adsorption of biomaterials onto its surface. [27][28][29] Here, the nanoreplica molding approach was used to replicate the 1D PC structure from a 555.5 nm-period silicon grating (LightSmyth Technologies) to an optical adhesive (NOA 86, Norland Products) on a 180 μm-thick plastic sheet. A 100 nm TiO 2 thin film was coated on the replicated grating using an electron beam evaporator.…”

Towards nanovesicle-based disease diagnostics: a rapid single-step exosome assay within one hour throughin situimmunomagnetic extraction and nanophotonic label-free detection

“…41 These membrane markers have been used to distinguish macrophage-derived EV subtypes. 42 Three different PC biosensors were functionalized using the anti-CD63, anti-CD9, and anti-CD81 capture antibodies via the PVAm/GA coupling reaction. After the coating of these antibodies, the non-specific bindings on the sensor surfaces were blocked using a bovine serum albumin (BSA)/GA composite.…”

Proteomic profiling in extracellular vesicles for cancer detection and monitoring

“…Their results showed that a five‐marker panel (epidermal growth factor receptor, epithelial cell adhesion molecule (EPCAM), mucin 1, glypican 1 and wnt family member 2) showed 84% accuracy for cancer detection, which was better than the measurement based on any single‐marker detection [108]. Similarly, a photonic crystal assay was used to recognize EV surface antigens and identify macrophage‐derived EV populations [109]. In label‐free EV arrays, no fluorescence signals are produced during immunobinding reactions instead the transmission spectral shift caused by affinity combination provides the basis of detection.…”