Hyperspectral chemical imaging reveals spatially varied degradation of polycarbonate urethane (PCU) biomaterials

Dorrepaal, Ronan M.; Lawless, Bernard; Burton, Hanna E.; Espino, Daniel M.; Shepherd, Duncan E.T.; Gowen, Aoife

doi:10.1016/j.actbio.2018.03.045

Cited by 9 publications

(7 citation statements)

References 38 publications

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“…In conclusion, hard block degradability of carbonate-based hard block degradable polymers could be proven for the first time through nonmatching degradation speeds of BHPC_211 and BHPC_312. While PCUs based on carbonates in the soft block are often described as nondegradable, , BHPC_211 and BHPC_312 exhibited considerable degradability. Additionally, carbonates like BHPC are known to erode faster enzymatically than hydrolytically. , Therefore, it is expected that BHPC_211 will reach higher degradation rates in vivo than the degradation rates reported herein.…”

Section: Resultsmentioning

confidence: 99%

Hard Block Degradable Polycarbonate Urethanes: Promising Biomaterials for Electrospun Vascular Prostheses

et al. 2019

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We report biodegradable thermoplastic polyurethanes for soft tissue engineering applications, where frequently used carboxylic acid ester degradation motifs were substituted with carbonate moieties to achieve superior degradation properties. While the use of carbonates in soft blocks has been reported, their use in hard blocks of thermoplastic polyurethanes is unprecedented. Soft blocks consist of poly(hexamethylene carbonate), and hard blocks combine hexamethylene diisocyanate with the newly synthesized cleavable carbonate chain extender bis(3-hydroxypropylene)carbonate (BHPC), mimicking the motif of poly(trimethylene carbonate) with highly regarded degradation properties. Simultaneously, the mechanical benefits of segmented polyurethanes are exploited. A lower hard block concentration in BHPC-based polymers was more suitable for vascular grafts. Nonacidic degradation products and hard block dependent degradation rates were found. Implantation of BHPC-based electrospun degradable vascular prostheses in a small animal model revealed high patency rates and no signs of aneurysm formations. Specific vascular graft remodeling and only minimal signs of inflammatory reactions were observed.

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Section: Resultsmentioning

confidence: 99%

Hard Block Degradable Polycarbonate Urethanes: Promising Biomaterials for Electrospun Vascular Prostheses

et al. 2019

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“…Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy operates by measuring the changes that occur in an internally reflected infrared beam when the beam comes into contact with a sample. ATR-FTIR has been reported to investigate protein adsorption on biomaterials, surface segregation, and restructuring in poly( l -lactide) and poly( d , l -lactide- co -glycolide) films and degradation in polycarbonate urethane (PCU) biomaterials . Recently, Mukherjee et al applied ATR–FTIR chemical imaging to develop multivariate models for prediction of surface wettability of polymeric materials.…”

Section: Introductionmentioning

confidence: 99%

“…ATR-FTIR has been reported to investigate protein adsorption on biomaterials, 5 surface segregation, and restructuring in poly(L-lactide) and poly(D,Llactide-co-glycolide) films 6 and degradation in polycarbonate urethane (PCU) biomaterials. 7 Recently, Mukherjee et al 8 applied ATR−FTIR chemical imaging to develop multivariate models for prediction of surface wettability of polymeric materials. Good results were obtained with R 2 P of 0.98 and root-mean-square errors of prediction (RMSEP) around 5°w hen tested on an independent experimental set.…”

Section: Introductionmentioning

confidence: 99%

Predictive Modeling of the In Vitro Responses of Preosteoblastic MC3T3-E1 Cells on Polymeric Surfaces Using Fourier Transform Infrared Spectroscopy

Leśniak

Gowen

2020

ACS Appl. Mater. Interfaces

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Biomaterials' surface properties elicit diverse cellular responses in biomedical and biotechnological applications. Predicting the cell behavior on a polymeric surface is an ongoing challenge due to its complexity. This work proposes a novel modeling methodology based on attenuated total reflection−Fourier transform infrared (ATR−FTIR) spectroscopy. Spectra were collected on wetted polymeric surfaces to incorporate both surface chemistry and information on water−polymer interactions. Results showed that predictive models built with spectra from wetted surfaces ("wet spectra") performed much better than models built using spectra acquired from dry surfaces ("dry spectra"), suggesting that the water−polymer interaction is critically important to the prediction of subsequent cell behavior. The best model was seen to predict total area of focal adhesions with coefficient of determination for prediction (R 2 P ) of 0.94 and root-mean-square errors of prediction (RMSEP) of 4.03 μm 2 when tested on an independent experimental set. This work offers new insights into our understanding of cell−biomaterial interactions. The presence of carboxyl groups in polymers promoted larger adhesion areas, yet the formation of carbonyl-to-water interaction decreased adhesion areas. Surface wettability, which was related to the water−polymer interaction, was proven to highly influence cell adhesion. The good predictive ability opens new possibilities for high throughput monitoring of cell attachment on polymeric substrates.

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“…As 30–40% of patients do not have a viable vein for replacement [15], new replacement strategies may be important. Biomaterials, though, are subject to surface degradation [16]; however, surface properties are so far mostly ignored.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Mechanical Overloading on Surface Roughness of the Coronary Arteries

Burton

Espino

2019

Applied Bionics and Biomechanics

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Background. Surface roughness can be used to identify disease within biological tissues. Quantifying surface roughness in the coronary arteries aids in developing treatments for coronary heart disease. This study investigates the effect of extreme physiological loading on surface roughness, for example, due to a rupture of an artery. Methods. The porcine left anterior descending (LAD) coronary arteries were dissected ex vivo. Mechanical overloading was applied to the arteries in the longitudinal direction to simulate extreme physiological loading. Surface roughness was calculated from three-dimensional reconstructed images. Surface roughness was measured before and after damage and after chemical processing to dehydrate tissue specimens. Results. Control specimens confirmed that dehydration alone results in an increase of surface roughness in the circumferential direction only. No variation was noted between the hydrated healthy and damaged specimens, in both the longitudinal (0.91±0.26 and 1.05±0.25 μm) and circumferential (1.46±0.38 and 1.47±0.39 μm) directions. After dehydration, an increase in surface roughness was noted for damaged specimens in both the longitudinal (1.28±0.33 μm) and circumferential (1.95±0.56 μm) directions. Conclusions. Mechanical overloading applied in the longitudinal direction did not significantly affect surface roughness. However, when combined with chemical processing, a significant increase in surface roughness was noted in both the circumferential and longitudinal directions. Mechanical overloading causes damage to the internal constituents of the arteries, which is significantly noticeable after dehydration of tissue.

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Hyperspectral chemical imaging reveals spatially varied degradation of polycarbonate urethane (PCU) biomaterials

Cited by 9 publications

References 38 publications

Hard Block Degradable Polycarbonate Urethanes: Promising Biomaterials for Electrospun Vascular Prostheses

Hard Block Degradable Polycarbonate Urethanes: Promising Biomaterials for Electrospun Vascular Prostheses

Predictive Modeling of the In Vitro Responses of Preosteoblastic MC3T3-E1 Cells on Polymeric Surfaces Using Fourier Transform Infrared Spectroscopy

The Effect of Mechanical Overloading on Surface Roughness of the Coronary Arteries

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