Predictive Modeling of the In Vitro Responses of Preosteoblastic MC3T3-E1 Cells on Polymeric Surfaces Using Fourier Transform Infrared Spectroscopy

Abstract

Biomaterials' surface properties elicit diverse cellular responses in biomedical and biotechnological applications. Predicting the cell behavior on a polymeric surface is an ongoing challenge due to its complexity. This work proposes a novel modeling methodology based on attenuated total reflection−Fourier transform infrared (ATR−FTIR) spectroscopy. Spectra were collected on wetted polymeric surfaces to incorporate both surface chemistry and information on water−polymer interactions. Results showed that predictive models built with spectra from wetted surfaces ("wet spectra") performed much better than models built using spectra acquired from dry surfaces ("dry spectra"), suggesting that the water−polymer interaction is critically important to the prediction of subsequent cell behavior. The best model was seen to predict total area of focal adhesions with coefficient of determination for prediction (R 2 P ) of 0.94 and root-mean-square errors of prediction (RMSEP) of 4.03 μm 2 when tested on an independent experimental set. This work offers new insights into our understanding of cell−biomaterial interactions. The presence of carboxyl groups in polymers promoted larger adhesion areas, yet the formation of carbonyl-to-water interaction decreased adhesion areas. Surface wettability, which was related to the water−polymer interaction, was proven to highly influence cell adhesion. The good predictive ability opens new possibilities for high throughput monitoring of cell attachment on polymeric substrates.

Systematically engineered graphene sheets with electrostatic Au-reinforcement to strengthen 2D nanofibrous scaffolds for improved bone regeneration

“…Typically, nano-fibrous mats have prepared using bio-compatible materials that help in the growth of tissues, cell proliferation, and differentiation. There are many natural polymers like chitosan, cellulose, collagen [10], chiton, and synthetic polymers like polycaprolactone (PCL), polyvinyl chloride, polypropylene, and polyurethanes are used in bone tissue engineering as per their bio-compatibility, water uptake ratio, mechanical strength, and other properties [11]. Among all, PCL is a semi-crystalline non-toxic polymer that is easily soluble in almost all kinds of aromatic polar solvents [12] and has excellent mechanical strength, biocompatibility, biodegradability, and easy availability which leads to its extensive use in the bio-medical field [13,14].…”

Physicochemical and Biological Characterisation of Diclofenac Oligomeric Poly(3-hydroxyoctanoate) Hybrids as β-TCP Ceramics Modifiers for Bone Tissue Regeneration

“…Complex interactions occurring at the cell-cell and cell-substrate levels affect the ability to adsorb proteins responsible for the regeneration of the appropriate tissue. However, an undoubted role in the abovementioned interactions is played by parameters relating to the surface characteristics-the as-mentioned wettability, surface energy, but also surface topography, its roughness as well as porosity and pore size distribution [66]. Taking into account only the topographic parameters of materials, i.e., roughness, it may have a different impact on the behaviour of living cells in the tested models [21].…”

Characterisation and Classification of Foodborne Bacteria Using Reflectance FTIR Microscopic Imaging

“…In a modern food-processing scenario, real-time monitoring procedures are desired to make timely corrective action. Vibrational spectroscopy coupled with machine learning algorithms has the potential to meet the requirements of being rapid, sensitive, and non-destructive [ 6 ]. Fourier transforms infrared spectroscopy (FTIR) probes structural information of biological molecules including carbohydrates, proteins, and lipids; therefore it has been proven to be useful in the analysis of bacteria [ 7 , 8 ].…”