Hypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX

Tomczyńska, Iga; Jupe, Florian; Hein, Ingo; Marczewski, Waldemar; Śliwka, Jadwiga

doi:10.1007/s11032-014-0050-2

Cited by 24 publications

(18 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Differences were assumed to be statistically significant at p value ≤ 0.05. dpi: days post-inoculation; hpi: hours post-inoculation; post-inoculation; 168a: stu-miR168a; 162: stu-miR162 (PotatoMir1005244514_x19366); 482_16170: PotatoMir1005658171_x16170; 482_15854: PotatoMir1005353123_x15854; 172e: stu-miR172e; AGO1-2 (PGSC0003DMC400054073): isoform 2 of Argonaute 1; DCL1 (GSC0003DMT400029301): endoribonuclease Dicer homologue 1; TOE3 (CK265044): apetala2-like ethylene-responsive transcription factor TOE3-like; Gpa2 (PGSC0003DMT400050510): disease resistance protein Gpa2; Cc-nbs-lrr (PGSC0003DMT400019599): cc-nbs-lrr resistance protein, JHL06P13.14 protein. *Statistically significant differences compared with the control (p value ≤ 0.05) both 20 and 28°C (Tomczyńska et al 2014). In this study, the potato diploid clone DG 81-68 exhibited necrotic lesions in both the inoculated leaves and the non-inoculated upper leaves to PVY infection at 28°C.…”

Section: Discussionmentioning

confidence: 44%

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

2019

Self Cite

View full text Add to dashboard Cite

We demonstrate temporal-, spatial-and temperature-dependent expression of five host microRNAs and target mRNAs in potato leaves after potato virus Y (PVY) inoculation. A novel gene, Ny-DG, conferring resistance to PVY was mapped on chromosome IX in the diploid potato clone DG 81-68. At 20°C, the PVY NTN strain was localized in the inoculated leaves without symptoms. At 28°C, necrotic lesions were observed in both the inoculated leaves and the non-inoculated upper leaves and the virus spread systemically, which indicates overcoming the resistance. At 0.5 h post-inoculation and 3-day postinoculation (dpi), PVY led to significant changes in the levels of the tested miRNAs and their targets only in a small number of cases. At 6 dpi, in the inoculated leaves at 28°C, the increased expression of stu-miR162, stu-miR168a and stu-miR482 (PotatoMir1005353123_x15854) promoted the downregulation of their targets DCL1, AGO1-2 and Cc-nbs-lrr, respectively. The expression of stu-miR482 (PotatoMir1005658171_x16170) and stu-miR172e remained unchanged, whereas their targets Gpa2 and TOE3 were downregulated. However, in the inoculated leaves at 20°C, all five tested miRNAs and their targets showed parallel downregulation. In the noninoculated upper leaves, changes in gene expression levels, namely downregulation, were only detected for the tested five miRNAs at 28°C. We conclude that the expression patterns of the tested miRNAs and targets were altered differently not only at different time points post-inoculation but also in the inoculated and upper leaves, and their accumulation levels were related to the type of reaction, which in turn was dependent on temperature.

show abstract

Section: Discussionmentioning

confidence: 44%

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…This protocol was used for identifying recombinant genotypes in the CL segregating population developed to accomplish the fine mapping of the Ry adg locus (Jara Vidalon 2010). However, the specificity of this multiplex Ry adg markers should be characterized using cultivars with other PVY resistance genes from S stoloniferum such as those mentioned in Van Eck et al (2017), Tomczyńska et al (2014), Mori et al (2011), and Szajko et al (2008, 2014). …”

Section: Discussionmentioning

confidence: 99%

“…Ny - Smira gene from cultivar Sarpo Mira was mapped to a location on the chromosome IX corresponding to Ry chc and Ny - 1 genes for PVY resistance. (Tomczyńska et al 2014). Recently, Ny (o,n)sto gene from S. stoloniferum was identified in potato cultivars in a position corresponding to the Ry sto on chromosome XI described by Brigneti et al (1997) (Van Eck et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Molecular and genetic characterization of the Ryadg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y

et al. 2018

View full text Add to dashboard Cite

Key messageWe have elucidated the Andigena origin of the potato Ryadg gene on chromosome XI of CIP breeding lines and developed two marker assays to facilitate its introgression in potato by marker-assisted selection.AbstractPotato virus Y (PVY) is causing yield and quality losses forcing farmers to renew periodically their seeds from clean stocks. Two loci for extreme resistance to PVY, one on chromosome XI and the other on XII, have been identified and used in breeding. The latter corresponds to a well-known source of resistance (Solanum stoloniferum), whereas the one on chromosome XI was reported from S. stoloniferum and S. tuberosum group Andigena as well. To elucidate its taxonomic origin in our breeding lines, we analyzed the nucleotide sequences of tightly linked markers (M45, M6) and screened 251 landraces of S. tuberosum group Andigena for the presence of this gene. Our results indicate that the PVY resistance allele on chromosome XI in our breeding lines originated from S. tuberosum group Andigena. We have developed two marker assays to accelerate the introgression of Ryadg gene into breeding lines by marker-assisted selection (MAS). First, we have multiplexed RYSC3, M6 and M45 DNA markers flanking the Ryadg gene and validated it on potato varieties with known presence/absence of the Ryadg gene and a progeny of 6,521 individuals. Secondly, we developed an allele-dosage assay particularly useful to identify multiplex Ryadg progenitors. The assay based on high-resolution melting analysis at the M6 marker confirmed Ryadg plex level as nulliplex, simplex and duplex progenitors and few triplex progenies. These marker assays have been validated and can be used to facilitate MAS in potato breeding.

show abstract

“…Маркер RAPD38-530 гена Rychc может проявлять специфичность не только к этому гену, но и в отношении генов гиперчувствительного типа (Ny). Созданный на его основе маркер Ry186 оказался пригодным для выявления гена Nysmira, происходящего, скорее всего, от S. tuberosum и впервые выявленного в сорте Sarpo Mira (Tomczyńska et al, 2014).…”

Section: Discussionunclassified

“…Также редким оказался маркер Ry186, способный выявлять гены устойчивости к PVY Rychc от S. chacoense и Ny-smira от S. tuberosum ssp. tuberosum (Tomczyńska et al, 2014). Он обнаружен лишь в восьми СЛ (2,1% коллекции).…”

Section: результатыunclassified

Marker assisted selection of potato breeding lines with combination of PVY resistance genes from different wild species

Воронкова

RUSETSKIY

Лукша

et al. 2020

Biotehnologiâ i selekciâ rastenij

View full text Add to dashboard Cite

Potato virus Y (PVY) is considered as one of the most harmful virus infections of this crop. Thus, it is a topical problem to breed potato varieties resistant against a wide range of PVY strains and to create initial breeding material that will have a combination of resistance genes from different species. The aim of the study was: (1) to genotype a collection of 376 breeding lines (BL), developed from complex interspecific hybrids, using DNA markers of PVY resistance genes, (2) to identify accessions with markers of resistance genes from different species for subsequent use in marker assisted selection (MAS), (3) to evaluate the suitability of DNA markers of PVY resistance genes for genotyping BL developed through interspecific hybridization. It was ascertained that the markers most widely represented in the collection were RYSC3 of the Ryadg gene (49.7%), Ry364 and RAPD38-530 of the Rychc gene (50.5% and 45.2%, respectively), and Yes3-3A of the Rysto gene (29.8%). The markers Ry186 of Rychc and GP122/EcoRV780 of Ryf-sto were found only in some accessions. The frequency of occurrence of BL that had markers of PVY resistance genes from two different species varied between 2.7% (Yes3-3a marker of Rysto and both two markers of Rychc) and 8.5-9.0% (RYSC3 marker of Ryadg and both two markers of Rychc, or only Ry364 marker of this gene). In total, the collection was found to contain 134 BL (47.6%) with markers of resistance genes from two different species. A combination of four markers for three genes of different origin (Ryadg, Rysto and Rychc) was found in 27 BL (7.2%). Extreme resistance to PVY of most BL (302 out of 357) was obviously determined by the presence in them of the currently used resistance genes detected by DNA markers applied in the study. Nevertheless, a significant part of accessions (55 of 61) that did not have any markers was resistant to PVY. At the same time, 13 BL (3.5%) with the markers were susceptible to the virus. Such a level of discrepancies is considered as acceptable for the initial MAS of breeding material. The obtained data on the presence of the markers of PVY resistance genes of different origin and their combination in BL ensures a more effective use of such BL in breeding in comparison with the BL resistant to the virus, though lacking corresponding markers.

show abstract

Hypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX

Cited by 24 publications

References 43 publications

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

Molecular and genetic characterization of the Ryadg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y

Marker assisted selection of potato breeding lines with combination of PVY resistance genes from different wild species

Contact Info

Product

Resources

About