Molecular and genetic characterization of the Ryadg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y

Herrera, Mariana; Vidalon, Laura Jara; Montenegro, Juan D.; Riccio, Cinzia; Guzmán, Frank; Bartolini, Ida; Ghislain, Marc

doi:10.1007/s00122-018-3123-5

Cited by 35 publications

(23 citation statements)

References 49 publications

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“…Emma genotyping as resistant using both markers yet is phenotypically classified as susceptible to PVY. Low association between RYSC3 marker alleles and Ry adg mediated resistance was also reported by Dalla Rizza et al [26] and Herrera et al [27]. To this effect, use of the RYSC3 marker has been replaced by alternative markers such as M45 and M6 [23,27].…”

Section: Introductionmentioning

confidence: 87%

“…Low association between RYSC3 marker alleles and Ry adg mediated resistance was also reported by Dalla Rizza et al [26] and Herrera et al [27]. To this effect, use of the RYSC3 marker has been replaced by alternative markers such as M45 and M6 [23,27]. There is currently no formal evidence in the literature to suggest that the M6 marker incorrectly genotypes any samples, however, it has only recently been reported and has not been subjected to close scrutiny or evaluation.…”

Section: Introductionmentioning

confidence: 96%

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Genome Enhanced Marker Improvement for Potato Virus Y Disease Resistance in Potato

et al. 2021

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Potato is an important food crop worldwide and is grown in a large number of countries. As such, the crop is under disease pressures and the need for selecting disease resistance genes during breeding programs is essential. Of particular importance within Australia and other parts of the world is the potyvirus, Potato virus Y (PVY). In this paper, three commonly used PVY resistance markers, M45, RYSC3 and M6, were evaluated using existing genomic resources and phenotypic data from the Australian potato breeding program to identify a region where the PVY resistance gene, Ryadg may reside. A region of Chromosome XI was investigated, and a cluster of disease resistance genes was identified that the resistance gene Ryadg is suspected to reside within. Protein characterization was also performed on the putative resistant gene. A specific variant that had complete association with the resistance gene was identified and a single nucleotide polymorphism (SNP) assay was designed to avoid dissociation of marker and gene in future breeding programs. This SNP marker (SNP37279) was validated as a Kompetitive Allele-specific PCR (KASP) genotyping assay and was found to perform more accurately than all previously used markers for detecting Ryadg.

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Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 96%

Genome Enhanced Marker Improvement for Potato Virus Y Disease Resistance in Potato

et al. 2021

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“…Wild potato varieties and landraces are sources of PVY-specific NLR resistance genes that can be introgressed into commercial potato cultivars. Loci conferring extreme resistance to PVY have been mapped in Solanum chacoense (Ry chc ), S. tuberosum group Andigena (Ry adg ), and S. stoloniferum (Ry sto ; Herrera et al, 2018). Grech-Baran et al (2020) employed resistance enrichment sequencing (RenSeq) to isolate the gene conferring Ry sto -mediated extreme resistance from the commercial potato cultivar, Alicja.…”

Section: Genes Conferring Extreme Resistance To Potato Virus Y Rely On Conserved Proteins That Are Also Necessary For Hrmentioning

confidence: 99%

Extreme Resistance to Viruses in Potato and Soybean

2021

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Plant pathogens, including viruses, negatively impact global crop production. Plants have evolved complex immune responses to pathogens. These responses are often controlled by nucleotide-binding leucine-rich repeat proteins (NLRs), which recognize intracellular, pathogen-derived proteins. Genetic resistance to plant viruses is often phenotypically characterized by programmed cell death at or near the infection site; a reaction termed the hypersensitive response. Although visualization of the hypersensitive response is often used as a hallmark of resistance, the molecular mechanisms leading to the hypersensitive response and associated cell death vary. Plants with extreme resistance to viruses rarely exhibit symptoms and have little to no detectable virus replication or spread beyond the infection site. Both extreme resistance and the hypersensitive response can be activated by the same NLR genes. In many cases, genes that normally provide an extreme resistance phenotype can be stimulated to cause a hypersensitive response by experimentally increasing cellular levels of pathogen-derived elicitor protein(s). The molecular mechanisms of extreme resistance and its relationship to the hypersensitive response are largely uncharacterized. Studies on potato and soybean cultivars that are resistant to strains of Potato virus Y (PVY), Potato virus X (PVX), and Soybean mosaic virus (SMV) indicate that abscisic acid (ABA)-mediated signaling and NLR nuclear translocation are important for the extreme resistance response. Recent research also indicates that some of the same proteins are involved in both extreme resistance and the hypersensitive response. Herein, we review and synthesize published studies on extreme resistance in potato and soybean, and describe studies in additional species, including model plant species, to highlight future research avenues that may bridge the gaps in our knowledge of plant antiviral defense mechanisms.

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“…Marker assisted selection can also screen at early stage major genes and Quantitative Trait Loci (QTL) with large effects (Gebhardt 2013;Sharma et al 2014). In recent years, markers flanking major genes and QTL were developed for resistance to viruses (Mihovilovich et al 2014;del Rosario et al 2018), tuber starch and yield (Schönhals et al 2016), and other important traits (Ramakrishnan et al 2015). If applied at the early clonal generation stage and multiplexed, marker-assisted selection can be cost-effective (Slater et al 2013).…”

Section: The Numerous Challenges Of Tetraploid Potato Breedingmentioning

confidence: 99%

The Genes and Genomes of the Potato

Ghislain

Douches

2020

The Potato Crop

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During the last decade, genomics research has generated new insights into potato genetics and made possible new strategies for varietal improvement. The most commonly grown and eaten potato is an autotetraploid, highly heterozygote crop suffering from rapid inbreeding depression. The genetic improvement of the potato presents numerous challenges using conventional tetraploid breeding techniques. However, novel breeding technologies are now available to increase precision and gains for varietal improvement. The public availability of the first potato genome sequence has created new ways to identify the genetic determinants of key traits of the potato as well as ways to use this knowledge for speeding up variety development. Genomic selection applied to tetraploid breeding promises to increase prediction of progeny performance by a more efficient selection of parents. Diploid hybrid breeding is finally making its way two decades after discovering a suppressor gene of the self-incompatibility locus of diploid potatoes. Direct gene transfer into existing varieties of major genes for key traits has been successful but biotech potato development has been constrained by public perception and issues related to the regulation of the technology. Although genome or gene editing is still in its primary stage in potato, it has already been successful in modifying gene expression in a controlled way, and it might face a lower regulatory burden and easier adoption than biotech, transgenic potatoes. Concluding on an optimistic note, we have many reasons, and evidence is starting to mount, that potato crop improvement is finally benefiting from decades of investment in molecular genetics and that the future hold the promises of faster releases of more robust varieties to pest, disease, and climatic extremes, as well as nutritionally enhanced varieties to feed an ever-growing world population.

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Molecular and genetic characterization of the Ryadg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y

Cited by 35 publications

References 49 publications

Genome Enhanced Marker Improvement for Potato Virus Y Disease Resistance in Potato

Genome Enhanced Marker Improvement for Potato Virus Y Disease Resistance in Potato

Extreme Resistance to Viruses in Potato and Soybean

The Genes and Genomes of the Potato

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