Hyperforin Suppresses Oncogenic Kinases and Induces Apoptosis in Colorectal Cancer Cells

Abstract

Background/Aim: Signal transducer and activator of transcription 3 (STAT3), Janus Kinase 1 (JAK1), extracellular signal-regulated kinase (ERK), and protein kinase B (AKT) are essential for malignant transformation and progression in colorectal cancer (CRC) and can be considered as targets for therapeutic interventions. Hyperforin, an active constituent from Hypericum perforatum, has been reported to inhibit inflammation. However, whether hyperforin may suppress CRC progression via inactivation of JAK/STAT3, ERK or AKT signaling remains unclear. Materials and Methods: Human CRC cells were used to identify the treatment efficacy of hyperforin and its underlying mechanisms of action by MTT, flow cytometry, wound healing, and western blotting assays. Results: Hyperforin not only induced cytotoxicity, extrinsic/intrinsic apoptosis signaling, but also suppressed the invasion/migration ability of CRC. The phosphorylation of STAT3, JAK1, ERK and AKT was found to be decreased by hyperforin. Conclusion: Hyperforin inactivates multiple oncogenic kinases and induces apoptosis signaling in CRC cells.

Accessing Apoptosis Induction and Metastasis Inhibition Effect of Magnolol on Triple Negative Breast CancerIn Vitro

“…MDA-MB-231and 4T1 cells were seeded in 96-well plates, at a density of 5×10 3 cells/well overnight and treated with magnolol 0, 20, 40, 60, 80, and 100 μM for 48 h. The medium was replaced with 100 μl MTT reagent (0.5 mg/ml) for 2 h. Then, the MTT medium was replaced with 100 μl DMSO. The absorbance was measured using a Multiskan FC microplate reader (Thermo Fisher Scientific) at 570 nm (19,20).…”

“…MDA-MB-231and 4T1 cells were plated in 6 well plates at a density of 2×10 5 cells/well, incubated overnight and treated with 0, 80, and 100 μM magnolol for 48 h. MDA-MB-231 and 4T1 cells were stained using cleaved caspase-3, caspase-8, and caspase-9 staining kit (CaspGLOW™ fluorescein staining kit, BioVision, Milpitas, CA, USA) incubate for 0.5 h at 37˚C incubator following treatment. The activation of cleaved caspase-3, caspase-8, and caspase-9 after treatment were analyzed in the FL-1 channel using the NovoCyte flow cytometer with NovoExpress ® system (Agilent Technologies Inc., Santa Clara, CA, USA) and the FlowJo (version 7.6.1) software for quantification (19,21).…”