Hyperbranched polymers for controlled release of cisplatin

Haxton, Katherine J.; Burt, Helen M.

doi:10.1039/b809949a

Cited by 56 publications

(53 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spectra also exhibited two pronounced peaks at 3292 and 1385 cm -1 , revealing the absorptions of N-H stretching from cisplatin ligands (Pt-NH 2 ), and the chelation of the COO-Pt stretch, respectively [47,48]. In addition, a reduction in absorbance of the carbonyl (C=O) stretch of COOH at 1704 cm -1 relative to the amide C=O band at 1633 cm -1 (the difference between relative intensity in (a) and (b) in Figure 11) is consistent with the prediction that some of the -COOH groups would react with the Pt atoms in a ligand exchange reaction [49]. Dynamic light scattering was also employed to confirm size changes in the polymer induced by the conjugation (Figure 12).…”

Section: Preparation Of the Cisplatin-loadedsupporting

confidence: 72%

Microwave-assisted synthesis of thermo- and pH-responsive antitumor drug carrier through reversible addition–fragmentation chain transfer polymerization

Wang¹,

Zheng²,

Chang³

et al. 2017

Express Polym. Lett.

View full text Add to dashboard Cite

Abstract. This paper reports the rapid synthesis of a dual-responsive copolymer through reversible addition-fragmentation chain transfer (RAFT) polymerization under microwave irradiation. Through use of 2-ethoxycarbonothioylthio acetic acid (ECTA) as a RAFT agent, the microwave-assisted polymerization rate of N-isopropylacrylamide (NIPAM) was approximately 150 times faster than that observed under conventional heating conditions, and the resulting homopolymer can be reactivated as a macroinitiator to produce poly(N-isopropylacrylamide-block-methacrylic acid) (PNIPAM-b-PMAA) block copolymers through a similar method. Research into the detailed polymerization kinetics of the PNIPAM and PNIPAM-b-PMAA revealed living characteristics that included a linear relationship between M n and conversion, controlled molecular weights, and a relatively narrow molecular weight distribution. The solution of the block copolymers in phosphate-buffered saline buffer displayed a phase transition at a lower critical solution temperature transition of 42°C, and altering the pH from 7 to 3.5 resulted in various degrees of polymer aggregation in the solution. Cisplatin was loaded to the polymeric carrier through a ligand exchange to form a macromolecular prodrug. The observed critical micelle concentration was 0.25 mg/mL. Overall, these polymers offer considerable potential for developing a new multifunctional drug delivery system.

show abstract

Section: Preparation Of the Cisplatin-loadedsupporting

confidence: 72%

Microwave-assisted synthesis of thermo- and pH-responsive antitumor drug carrier through reversible addition–fragmentation chain transfer polymerization

Wang¹,

Zheng²,

Chang³

et al. 2017

Express Polym. Lett.

View full text Add to dashboard Cite

show abstract

“…Moreover, such system can deliver simultaneously two or more drugs for the combination therapy [16,17]. It was reported that linear water-soluble polymers, block copolymers able to form micelles [13][14][15][16][17], and branched polymers have been studied as nanocarriers for chemotherapeutic agents [18]. Such nanosystems containing cis-Pt can be efficiently accumulated in the tumors, thereby enhancing the antitumor action.…”

Section: Introductionmentioning

confidence: 99%

Aggregation Processes in Hybrid Nanosystem Polymer/Nanosilver/Cisplatin

Kutsevol¹,

Naumenko²,

Chumachenko³

et al. 2018

Ukr. J. Phys.

View full text Add to dashboard Cite

AGGREGATION PROCESSES IN HYBRID NANOSYSTEM POLYMER/NANOSILVER/CISPLATINHybrid nanosystems consisting of star-like copolymer Dextran-graft-Polyacrylamide in the anionic form (D-g-PAA(PE)), silver nanoparticles (AgNPs), and cisplatin (cis-Pt) have been synthesized in water and characterized by TEM, DLS, FTIR, and UV-Vis spectroscopies. It is shown that cis-Pt forms a complex with carboxylate groups of the polymer. For the ternary system Polymer/AgNPs/cis-Pt, a change in the hydrophilic-hydrophobic balance of a polymer molecule (due to the complexation with cis-Pt) and the aggregation of macromolecules, as well as to some agglomeration AgNPs, are revealed. The decrease of the antitumor efficiency of the hybrid ternary nanosystem Polymer/AgNPs/cis-Pt in comparison with the Polymer/cis-Pt system is discussed.K e y w o r d s: silver nanoparticles, branched polymer, polyelectrolyte, cisplatin, aggregation.

show abstract

“…These dendrimers have included PAMAM, polyglycerol [22], poly(propyleneimine) [23], and thiol-yne derived [21] dendrimers with the active components of both cisplatin [17] and oxaliplatin [16] and a novel DNA intercalating platinum complex [15]. In some cases the platinum drugs are attached non-reversibly to the dendrimer through coordination to the amine groups, but in most cases the drugs were attached either by electrostatic attraction [15], or through reversible but direct coordination of the platinum atom to the terminal carboxylate groups [16][17].…”

Section: Introductionmentioning

confidence: 99%

“…Previously dendrimers have been examined sporadically as delivery vehicles for several different types of platinum drugs and complexes [15][16][17][18][19][20][21][22]. These dendrimers have included PAMAM, polyglycerol [22], poly(propyleneimine) [23], and thiol-yne derived [21] dendrimers with the active components of both cisplatin [17] and oxaliplatin [16] and a novel DNA intercalating platinum complex [15].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of anionic half generation 3.5–6.5 poly(amidoamine) dendrimers as delivery vehicles for the active component of the anticancer drug cisplatin

Kirkpatrick

Plumb

Sutcliffe

et al. 2011

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

Title: Evaluation of anionic half generation 3.5-6.5 poly(amidoamine) dendrimers as delivery vehicles for the active component of the anticancer drug cisplatin Article Type: Regular Paper Keywords: cancer; platinum; PAMAM dendrimer; cisplatin; guanosine; cytotoxicity; diffusion NMR. Abstract: Aquated cisplatin was added to half-generation PAMAM dendrimers and the resultant complexes were purified by centrifuge. The drug-dendrimer complexes were then characterised by 1-D and diffusion 1H NMR y and ICP-AES. The amount of drug bound was found to increase in proportion with dendrimer size: G3.5, 22 cis-{Pt(NH3)2}molecules per dendrimer; G4.5, 37; G5.5, 54; and G6.5, 94, which represent only a fraction of the available binding sites on each dendrimer (68, 58, 42 and 37%, respectively). Drug release studies showed that some drug remains bound to the dendrimer even after prolonged incubation with 5'GMP at temperatures of 60 oC for over a week (percentage of drug released 18, 30, 35 and 63%, respectively). Attachment of the drug was found to decrease the radius of the dendrimers. Finally, the effect of the dendrimer on drug cytotoxicity was determined using in vitro assays with the A2780, A2780cis and A2780cp ovarian cancer cell lines. The free dendrimers display no cytotoxicity whilst the drug-dendrimer complexes showed moderate activity. In vivo activity was examined using an A2780 tumour xenograft. Cisplatin, at its maximum tolerated dose of 6 mg/kg, reduced tumour size by 33% compared to an untreated control group. The G6.5 cisplatin-dendrimer complex was administered at two doses (6 and 8 mg/kg equivalent of cisplatin). Both were well tolerated by the mice. The lower dose displayed comparable activity to cisplatin with a tumour volume reduction of 32%, but the higher dose was significantly more active than free cisplatin with a tumour reduction of 45%. We have revised the manuscript taking into account all your listed corrections and stylistic requirements.As siDNA and PBS were used only once in the manuscript we have now written these in full and not used them as abbreviations.For simplicity, we have made figure 5 just black and white and have changed the figure caption to reflect this. G4.5, 37; G5.5, 54; and G6.5, 94, which represent only a fraction of the available binding sites on each dendrimer (68, 58, 42 and 37%, respectively). Drug release studies showed that some drug remains bound to the dendrimer even after prolonged incubation with 5'GMP at temperatures of 60 o C for over a week (percentage of drug released 18, 30, 35 and 63%, respectively). Attachment of the drug was found to decrease the radius of the dendrimers. Finally, the effect of the dendrimer on drug cytotoxicity was determined using in vitro assays with the A2780, A2780cis and A2780cp ovarian cancer cell lines. The free dendrimers display no cytotoxicity whilst the drug-dendrimer complexes showed moderate activity. In vivo activity was examined using an A2780 tumour xenograft. Cisplatin, at its maximum tolerated dose of 6 mg/kg, reduced tu...

show abstract

Hyperbranched polymers for controlled release of cisplatin

Abstract: Hyperbranched polymers based on a polyether and polyester have been modified and used as macromolecular ligands for the anticancer drug cisplatin, giving controlled release of the drug over 7 days.

Cited by 56 publications

References 14 publications

Microwave-assisted synthesis of thermo- and pH-responsive antitumor drug carrier through reversible addition–fragmentation chain transfer polymerization

Microwave-assisted synthesis of thermo- and pH-responsive antitumor drug carrier through reversible addition–fragmentation chain transfer polymerization

Aggregation Processes in Hybrid Nanosystem Polymer/Nanosilver/Cisplatin

Evaluation of anionic half generation 3.5–6.5 poly(amidoamine) dendrimers as delivery vehicles for the active component of the anticancer drug cisplatin

Contact Info

Product

Resources

About