Hyperbranched poly(glycidol)/poly(ethylene oxide) crosslinked hydrogel for tissue engineering scaffold using e‐beams

Abstract

A microporous hydrogel scaffold was developed from hyperbranched poly(glycidol) (HPG) and poly(ethylene oxide) (PEO) using electron beam (e-beam) induced cross-linking for tissue engineering applications. In this study, HPG was synthesized from glycidol using trimethylol propane as a core initiator and cross-linked hydrogels were made using 0, 10, 20, and 30% HPG with respect to PEO. The effects of %-HPG on the swelling ratio, cross-linking density, mechanical properties, morphology, degradation, and cytotoxicity of the hydrogel scaffolds were then investigated. Increasing the HPG content increased the pore size of the hydrogel scaffold, as well as the porosity, elongation at break, degree of degradation and swelling ratio. In contrast, the presence of HPG decreased the cross-linking density of the hydrogel. There was no significant difference in compressive modulus and tensile strength of all compositions. The pore size of hydrogel scaffolds could be easily tailored by controlling the content of HPG in the polymer blend. Evaluation of the cytotoxicity demonstrated that HPG/PEO hydrogel scaffold has potential for use as a matrix for cellular attachment and proliferation. These results indicate that cross-linked HPG/PEO hydrogel can function as a potential material for tissue engineering scaffolds. Moreover, a facile method to prepare hydrogel microporous scaffolds for tissue engineering by e-beam irradiation was developed.

A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine

“…Haryanto fabricated a hyerbranched poly (glycido)/poly (ethylene oxide) crosslinked hydrogel for tissue engineering scaffold. The hydrogel's tensile strength was measured using a tensile test machine (Instron 2710–105, USA) with a constant extension rate of 10 mm min −1 at room temperature . As mentioned above, these conventional detection methods are easy to handle and low cost.…”

“…The hydrogel's tensile strength was measured using a tensile test machine (Instron 2710-105, USA) with a constant extension rate of 10 mm min 21 at room temperature. 71 As mentioned above, these conventional detection methods are easy to handle and low cost. However, these methods also have certain limitations, such as hydrogel water loss and slippage often occurs during testing.…”

Self-Healable, Injectable Hydrogel with Enhanced Clotrimazole Solubilization as a Potential Therapeutic Platform for Gynecology

“…Unimolecular micelles, i.e., dendrimer-type, − star-shaped, or hyperbranched , amphiphilic macromolecules, in which all chains are covalently bound, overcome the limitations of standard micelles. Among hyperbranched polymers, hyperbranched polyglycidol, HbPGL, thanks to its biocompatibility, − low viscosity, high hydrophilicity, and numerous functional groups, according to us, deserves a particular interest in view of the formation of hydrogel-based carriers for gynecology applications. Diol groups present in the terminal units (approx.…”

Cross-linkable star-hyperbranched unimolecular micelles for the enhancement of the anticancer activity of clotrimazole

“…diagnostics combined with treatment. 35 The high biomedical potential of HbPGL results from its excellent biocompatibility [36][37][38][39][40][41][42] and low toxicity. 43 In addition, thanks to its better thermal and oxidative stability, HbPGL is considered an alternative to PEG.…”