Hyperbaric oxygen treatment suppresses withdrawal signs in morphine-dependent mice

Abstract

Hyperbaric oxygen (HBO2) therapy reportedly reduces opiate withdrawal in human subjects. The purpose of this research was to determine whether HBO2 treatment could suppress physical signs of withdrawal in opiate-dependent mice. Male NIH Swiss mice were injected s.c. with morphine sulfate twice a day for 4 days, the daily dose gradually increasing from 50 mg/kg on day 1 to 125 mg/kg on day 4. On day 5, withdrawal was precipitated by i.p. injection of 5.0 mg/kg naloxone. Mice were observed for physical withdrawal signs, including jumping, forepaw tremor, wet-dog shakes, rearing and defecation for 30 min. Sixty min prior to the naloxone injection, different groups of mice received either a 30-min or 60-min HBO2 treatment at 3.5 atmospheres absolute. HBO2 treatment significantly reduced naloxone-precipitated jumping, forepaw tremor, wet-dog shakes, rearing and defecation. Based on these experimental findings, we concluded that treatment with HBO2 can suppress physical signs of withdrawal syndrome in morphine-dependent mice.

The Potential of Hyperbaric Oxygen Therapy Against Codeine Addiction Reduction

“…On the other hand, it was discovered that HBOT therapy inhibited NOS, which resulted in the suppression of addiction symptoms although the nociceptive effects remained. This difference could imply that the effect of NO on the emergence of addiction is balanced, similar to the role of NO-induced pro-nociception and anti-nociception, anxiogenic and anxiolytic effects, and their involvement in neurotoxicity and neuroprotection [29]. Another study found that administering HBO decreases withdrawal effects in the rat brain through regulating monoaminergic neurotransmitters and the NO-cGMP signaling pathway, suggesting that HBO could be used as a therapeutic alternative for opioid addiction [28].…”

“…This difference balances the effects of NO on the development of addiction and is significant with their involvement in NO-induced nociception and anti-nociception, anxiogenic and anxiolytic functions, and neurotoxicity and neuroprotection. May suggest that it does not differ [29]. Another study suggests that administration of HBO involves the regulation of monoamine neurotransmitters and NOcGMP signaling pathways that suppress withdrawal symptoms in rat brain and may be a therapeutic option for opioid addiction [28].…”

The Chemical Profile and Potency of Albertisia papuana Leaves in Suppressing Withdrawal Symptoms of Morphine Addiction in Mice

“…Mice showing no signs of addiction were excluded from the study. Ethanol extracts of A. papuana leaves and a placebo were given for 14 days and on the last day of the study, the mice were observed using psychomotor tests, ear nerve sensitivity tests, light sensitivity tests, curiosity tests, and coordination behaviour tests [12][13][14][15][16][17][18][19][20]. RESULTS 79 g of A. papuana leaves were macerated and extracted with 96% ethanol.…”

Hyperbaric Oxygenation in the Treatment of Traumatic Brain Injury

“…Of no small importance is groundbreaking research from Washington State University. Researchers found that HBOT can halve the pain and symptoms of opiate withdrawal/detox [37].…”