Hyperbaric oxygen therapy improves age induced bone dyshomeostasis in non-obese and obese conditions

Imerb, Napatsorn; Thonusin, Chanisa; Pratchayasakul, Wasana; Arunsak, Busarin; Nawara, Wichwara; Aeimlapa, Ratchaneevan; Charoenphandhu, Narattaphol; Chattipakorn, Nipon

doi:10.1016/j.lfs.2022.120406

Cited by 6 publications

(15 citation statements)

References 52 publications

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“…These findings suggested that D-gal-induced aging and obesity extensively trigger the proinflammatory cascades, and therefore leading to increased inflammatory cytokines. Consistently, other previous studies also showed that both D-gal induced-aging rats and HFD-induced obese rats had higher levels of TNF-α, IL-1β, and IL-6 expression than those of the control group 35 – 37 . Taken together, all of these findings suggested that D-gal and HFD both independently and synergistically caused increased oxidative stress and bone inflammation, resulting in aging- and obesity-related bone metabolic diseases.…”

Section: Discussionsupporting

confidence: 90%

“…Aging and bone turnover markers in serum, including AGEs, P1NP, and CTX-I were measured using standard ELISA kits according to the manufacturer’s instructions. The details of each assay were described in a prior study 59 .…”

Section: Methodsmentioning

confidence: 99%

“…Serum sRAGE protein expression was determined using Western blot analysis. Each step of Western blot was detailed in a previous study 59 .…”

Section: Methodsmentioning

confidence: 99%

“…Serum and bone MDA levels were measured using high performance liquid chromatography (HPLC) as described in prior studies 59 – 62 .…”

Section: Methodsmentioning

confidence: 99%

“…Bone histomorphometry was performed in the right tibiae. The details of bone preparation and analysis were described in a previous study 59 , 65 .…”

Section: Methodsmentioning

confidence: 99%

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D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis

Imerb

Thonusin

Pratchayasakul

et al. 2022

Sci Rep

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We aimed to compare the time-course effect of D-galactose (D-gal)-induced aging, obesity, and their combined effects on bone homeostasis. Male Wistar rats were fed with either a normal diet (ND; n = 24) or a high-fat diet (HFD; n = 24) for 12 weeks. All rats were then injected with either vehicle or 150 mg/kg/day of D-gal for 4 or 8 weeks. Blood was collected to measure metabolic, aging, oxidative stress, and bone turnover parameters. Bone oxidative stress and inflammatory markers, as well as bone histomorphometry were also evaluated. Additionally, RAW 264.7 cells were incubated with either D-gal, insulin, or D-gal plus insulin to identify osteoclast differentiation capacity under the stimulation of receptor activator of nuclear factor κB ligand. At week 4, D-gal-induced aging significantly elevated serum malondialdehyde level and decreased trabecular thickness in ND- and HFD-fed rats, when compared to the control group. At week 8, D-gal-induced aging further elevated advanced glycation end products, increased bone inflammation and resorption, and significantly impaired bone microarchitecture in HFD-fed rats. The osteoclast number in vitro were increased in the D-gal, insulin, and combined groups to a similar extent. These findings suggest that aging aggravates bone dyshomeostasis in the obese condition in a time-dependent manner.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

“…Serum sRAGE protein expression was determined using Western blot analysis. Each step of Western blot was detailed in a previous study 59 .…”

Section: Methodsmentioning

confidence: 99%

“…Serum and bone MDA levels were measured using high performance liquid chromatography (HPLC) as described in prior studies 59 – 62 .…”

Section: Methodsmentioning

confidence: 99%

“…Bone histomorphometry was performed in the right tibiae. The details of bone preparation and analysis were described in a previous study 59 , 65 .…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations