Hyperbaric oxygen induces rapid protection against focal cerebral ischemia

Veltkamp, Roland; Siebing, Dirk A.; Heiland, Sabine; Schoenffeldt-Varas, Philip; Veltkamp, Claudia; Schwaninger, Markus; Schwab, Stefan

doi:10.1016/j.brainres.2005.01.006

Cited by 43 publications

(35 citation statements)

References 29 publications

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“…EF-5 distribution was examined after 2-hour MCAO in the present study because we had previously found an improvement of in vivo MRI surrogate parameters in this setting. 28 We and others have also shown that administration of HBO in the acute phase reduces infarct growth in transient ischemia in rats and mice. [27][28][29][30] Remarkably, hypoxic area of EF-5 fluorescence was significantly smaller in HBO-treated mice compared with air and NBO.…”

Section: Sun Et Al Hyperbaric Oxygen Reduces Ischemia-induced Hypoxiamentioning

confidence: 84%

“…28 We and others have also shown that administration of HBO in the acute phase reduces infarct growth in transient ischemia in rats and mice. [27][28][29][30] Remarkably, hypoxic area of EF-5 fluorescence was significantly smaller in HBO-treated mice compared with air and NBO. Topographic analysis of EF-5 fluorescence revealed that hypoxic regions were decreased in medial striatum and cortical ischemic border areas in HBO-treated mice, which represent the penumbra in this model.…”

Section: Sun Et Al Hyperbaric Oxygen Reduces Ischemia-induced Hypoxiamentioning

confidence: 84%

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Hyperbaric Oxygen Reduces Tissue Hypoxia and Hypoxia-Inducible Factor-1α Expression in Focal Cerebral Ischemia

Sun

Marti

Veltkamp

2008

Stroke

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Background and Purpose-The usefulness of hyperbaric oxygen (HBO) and normobaric hyperoxia in acute ischemic stroke is being reexplored because both improve outcome in experimental cerebral ischemia. However, even the basic mechanisms underlying oxygen therapy are poorly understood. We investigated the effect of both oxygen therapies on tissue hypoxia and on the transcription factor hypoxia-inducible factor-1␣. Methods-Mice were subjected to filament-induced middle cerebral artery occlusion for 2 hours. Twenty-five minutes after filament introduction, mice breathed normobaric air, normobaric 100% O 2 (normobaric hyperoxia), or 100% O 2 at 3 ata (HBO) for 95 minutes. Hypoxic regions were mapped on tissue sections after preischemic infusion of the in vivo hypoxia marker EF-5. Hypoxia-inducible factor-1␣ protein was measured after 2-hour middle cerebral artery occlusion using immunofluorescence and immunoblotting. Vascular endothelial growth factor expression was analyzed using in situ mRNA hybridization. Results-Severity of ischemia did not differ among groups. HBO (35.2Ϯ10.4 mm2 ) significantly reduced the area of EF-5-stained hypoxic regions in focal cerebral ischemia compared with normobaric hyperoxia (46.4Ϯ11.2 mm 2 ) and air (49.1Ϯ8 mm 2 , PϽ0.05, analysis of variance). Topographically, EF-5 fluorescence was decreased in medial striatum and in cortical ischemic border areas. Immunohistochemistry and immunoblotting revealed lower hypoxia-inducible factor-1␣ protein in the ischemic hemisphere of HBO-treated mice. Moreover, mRNA in situ hybridization showed lower expression of vascular endothelial growth factor in HBO and normobaric hyperoxia groups. Conclusions-Measurement of extrinsic and intrinsic markers of hypoxia revealed that HBO improves penumbral oxygenation in focal ischemia. Modification of the transcription factor hypoxia-inducible factor-1␣ and its downstream targets may be involved in effects of HBO.

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Section: Sun Et Al Hyperbaric Oxygen Reduces Ischemia-induced Hypoxiamentioning

confidence: 84%

Section: Sun Et Al Hyperbaric Oxygen Reduces Ischemia-induced Hypoxiamentioning

confidence: 84%

Hyperbaric Oxygen Reduces Tissue Hypoxia and Hypoxia-Inducible Factor-1α Expression in Focal Cerebral Ischemia

Sun

Marti

Veltkamp

2008

Stroke

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“…Animal experiments and clinical trials have shown that HBO performed within 6 h after HIBD may achieve favorable outcomes and promote the long-term neurological recovery (3)(4)(5)(6). In addition, numerous studies in China have demonstrated that HBO can reduce the disability and mortality of HIBD (7).…”

Section: Introductionmentioning

confidence: 99%

Effect of hyperbaric oxygen on lipid peroxidation and visual development in neonatal rats with hypoxia-ischemia brain damage

Chen

et al. 2016

Biomedical Reports

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Abstract. The aim of the present study was to investigate the effect of hyperbaric oxygen (HBO) on lipid peroxidation and visual development in a neonatal rat model of hypoxic-ischemic brain damage (HIBD). The rat models of HIBD were established by delayed uterus dissection and were divided randomly into two groups (10 rats each): HIBD and HBO-treated HIBD (HIBD+HBO) group. Another 20 rats that underwent sham-surgery were also divided randomly into the HBO-treated and control groups. The rats that underwent HBO treatment received HBO (0.02 MPa, 1 h/day) 24 h after the surgery and this continued for 14 days. When rats were 4 weeks old, their flash visual evoked potentials (F-VEPs) were monitored and the ultrastructures of the hippocampus were observed under transmission electron microscope. The levels of superoxide dismutase (SOD) and malonyldialdehyde (MDA) in the brain tissue homogenate were detected by xanthine oxidase and the thiobarbituric acid colorimetric method. Compared with the control group, the ultrastructures of the pyramidal neurons in the hippocampal CA3 area were distorted, the latencies of F-VEPs were prolonged (P<0.01) and the SOD activities were lower while the MDA levels were higher (P<0.01) in the HIBD group. No significant differences in ultrastructure, the latency of F-VEPs or SOD/MDA levels were identified between the HBO-treated HIBD group and the normal control group (P>0.05). HBO enhances antioxidant capacity and reduces the ultrastructural damage induced by hypoxic-ischemia, which may improve synaptic reconstruction and alleviate immature brain damage to promote the habilitation of brain function. IntroductionHypoxic-ischemic brain damage (HIBD) occurs at a rate of 3-5/1,000 full-term live births in developed countries and ≤10-fold higher in developing countries (1). Following hypoxia-ischemia (HI), ~45% of newborns succumb or have permanent cognitive impairments, potentially including cerebral palsy, and there are currently no effective therapies (2).Hyperbaric oxygen (HBO) therapy has been identified as a clinical therapy for HIBD for a number of years. Animal experiments and clinical trials have shown that HBO performed within 6 h after HIBD may achieve favorable outcomes and promote the long-term neurological recovery (3-6). In addition, numerous studies in China have demonstrated that HBO can reduce the disability and mortality of HIBD (7). However, there are concerns regarding the safety and validity of HBO treatment due to oxygen toxicity, which may increase the infarct area (3). A previous study demonstrated an increase in lipid and protein oxidation products in the lung tissues of rats accompanied by increased antioxidant enzyme activities when HBO was continued for >20 sessions (8). In addition to the lung being the entering site of hyperoxic injuries, CNS is mainly accepted as another important target for oxygen exposure in toxic amounts (9). However, whether HBO will exacerbate lipid peroxidation causing secondary injury to the brain or lead to neonatal pulmonary and retina...

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“…Most showed a benefit to animals treated with HBO. Models of stroke examined cerebral ischemia with and without reperfusion through either temporary [7, 10, 11, 13, 14, 16, 17,59,60,61,62,63,64,65] (table 2) or permanent [6, 49, 50,65,66,67,68] (table 3) occlusion of cerebral arteries. Timing of temporary occlusions ranged from 20 min [11] to 24 h [59].…”

Section: Animal Studiesmentioning

confidence: 99%

Hyperbaric Oxygen Therapy of Cerebral Ischemia

Helms

Whelan

Torbey³

2005

Cerebrovasc Dis

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Background: Hyperbaric oxygen (HBO) therapy of cerebral ischemia has been evaluated in a number of human and animal studies; however, there is presently no consensus on its efficacy. Methods: We present a review of animal and human studies on HBO therapy of cerebral ischemia as well as present potential mechanisms of action of HBO. Results: Animal studies of HBO have shown promise by reducing infarct size and improving neurologic outcome. HBO has also been shown to inhibit inflammation and apoptosis after cerebral ischemia. Early reports in humans also suggested benefit in stroke patients treated with HBO. Recent randomized, controlled human studies, however, have not shown benefit, although all were limited by small sample size. Important differences between animal and human studies suggest HBO might be more effective in stroke within the first few hours and at a pressure of 2–3 ATA. Conclusions: The clinical usefulness of HBO in the treatment of cerebral ischemia is not yet certain. Attention to emerging pathophysiologic data should be taken into consideration in design of any future clinical trials of HBO in acute ischemic stroke.

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Hyperbaric oxygen induces rapid protection against focal cerebral ischemia

Cited by 43 publications

References 29 publications

Hyperbaric Oxygen Reduces Tissue Hypoxia and Hypoxia-Inducible Factor-1α Expression in Focal Cerebral Ischemia

Hyperbaric Oxygen Reduces Tissue Hypoxia and Hypoxia-Inducible Factor-1α Expression in Focal Cerebral Ischemia

Effect of hyperbaric oxygen on lipid peroxidation and visual development in neonatal rats with hypoxia-ischemia brain damage

Hyperbaric Oxygen Therapy of Cerebral Ischemia

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