Hyperacute Form of Experimental Autoimmune Uveo-Retinitis in Lewis Rats; Electron Microscopic Study

Interphotoreceptor Retinoid Binding Protein Peptide-induced Uveitis in B10.RIII Mice: Characterization of Disease Parameters and Immunomodulation

“…This immunization protocol requires no PTX to achieve reliable disease and is consistent with a previous study, which reports that PTX is not necessary for EAU induction in the B10.RIII (Silver et al, 1999). The B10.RIII model of EAU described here has similarities with S-Ag induced EAU in the Lewis rat, namely the acute nature of disease and retinal damage (de Kozak, Thillaye and Faure, 1978;de Kozak et al, 1981). The peptide model in the B10.RIII mice at a dose of 25 mg appears to be of an acute and highly damaging nature, which is largely uncharacteristic of human forms of posterior uveitic conditions.…”

“…Further demonstrations of this have been shown in the rat models of EAU e.g. Lewis, PVG and Lister black hooded rats, whereby varying doses of retinal antigens, results in different forms of disease, ranging from acute to a more moderate chronic form (de Kozak et al, 1978(de Kozak et al, , 1981Marak and Rao, 1982;Fox et al, 1987). However, it appears from this present study and previous reports (Silver et al, 1995) that B10.RIII mice have a predilection to develop acute disease with the IRBP 161±180 peptide.…”

Comparison of Aqueous and Vitreous Lymphocyte Populations From Two Rat Models of Experimental Uveitis

“…As a model of autoimmune uveitis, EAU is dependent on T cells 11 and generates an intraocular infiltrate that is T-cell and macrophage predominant. 12 – 16 As a mixed model of inflammation, PMU eyes shows CD68 + macrophages as the predominant cell type on histologic sections, with a relatively small T-cell (CD3 + ) infiltrate. 8 However, these analyses were histochemical and did not utilize flow cytometric analysis of live cells to quantitate the relative proportions of these populations.…”