Comparison of Aqueous and Vitreous Lymphocyte Populations From Two Rat Models of Experimental Uveitis

Pepple, Kathryn L.; Wilson, Leslie; Gelder, Russell N. Van

doi:10.1167/iovs.18-24192

Cited by 23 publications

(14 citation statements)

References 36 publications

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“…Our data suggests that the CD19-cre:ROSA-LUC could be a useful tool for studying B cells dynamics in the EAU model. Furthermore the strong bioluminescence signal from PMU CD19-cre:ROSA-LUC eyes suggests that B cells play a more general role in uveitis than previously appreciated since PMU and EAU are mechanistically distinct forms of EAU 2 . Further studies to understand the role of B cells, and possibly plasma cells, in the mechanisms of PMU are warranted.…”

Section: Discussionmentioning

confidence: 95%

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Bioluminescence for in vivo detection of cell-type-specific inflammation in a mouse model of uveitis

John

Rolnick

Wilson

et al. 2020

Sci Rep

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This study reports the use of cell-type-specific in vivo bioluminescence to measure intraocular immune cell population dynamics during the course of inflammation in a mouse model of uveitis. Transgenic lines expressing luciferase in inflammatory cell subsets (myeloid cells, T cells, and B cells) were generated and ocular bioluminescence was measured serially for 35 days following uveitis induction. Ocular leukocyte populations were identified using flow cytometry and compared to the ocular bioluminescence profile. Acute inflammation is neutrophilic (75% of ocular CD45 + cells) which is reflected by a significant increase in ocular bioluminescence in one myeloid reporter line on day 2. By day 7, the ocular T cell population increases to 50% of CD45 + cells, leading to a significant increase in ocular bioluminescence in the T cell reporter line. While initially negligible (< 1% of CD45 + cells), the ocular B cell population increases to > 4% by day 35. This change is reflected by a significant increase in the ocular bioluminescence of the B cell reporter line starting on day 28. Our data demonstrates that cell-type-specific in vivo bioluminescence accurately detects changes in multiple intraocular immune cell populations over time in experimental uveitis. This assay could also be useful in other inflammatory disease models.

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Section: Discussionmentioning

confidence: 95%

“…Post mortem assays such as histology, immunohistochemistry, and flow cytometry are the current gold standard assays for identifying immune cells in eyes with experimental uveitis [1][2][3] . While these are powerful tools and can provide high resolution information about the infiltrating cell populations, they are all terminal assays.…”

mentioning

confidence: 99%

Bioluminescence for in vivo detection of cell-type-specific inflammation in a mouse model of uveitis

John

Rolnick

Wilson

et al. 2020

Sci Rep

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“…Experimental autoimmune uveitis (EAU) is the most widely studied disease model for human non-infectious uveitis (24). In EAU, immune responses targeting the neural retina and related tissue are mainly induced by T cells (25,26).…”

Section: Experimental Models For Non-infectious Uveitismentioning

confidence: 99%

A Review of the Various Roles and Participation Levels of B-Cells in Non-Infectious Uveitis

Zhu

Chen

2021

Front. Immunol.

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Non-infectious uveitis is an inflammatory disorder of the eye that accounts for severe visual loss without evident infectious agents. While T cells are supposed to dominate the induction of inflammation in non-infectious uveitis, the role of B cells in the pathogenesis of this disease is obscure. Therefore, this review aimed to discuss diverse B-cell participation in different non-infectious uveitides and their roles in the pathogenesis of this disease as well as the mechanism of action of rituximab. Increasing evidence from experimental models and human non-infectious uveitis has suggested the participation of B cells in non-infectious uveitis. The participation levels vary in different uveitides. Furthermore, B cells play multiple roles in the pathogenic mechanisms. B cells produce autoantibodies, regulate T cell responses via antibody-independent functions, and constitute ectopic lymphoid structures. Regulatory B cells perform pivotal anti-inflammatory functions in non-infectious uveitis. Rituximab may work by depleting pro-inflammatory B cells and restoring the quantity and function of regulatory B cells in this disease. Identifying the levels of B-cell participation and the associated roles is beneficial for optimizing therapy. Diversified experimental model choices and emerging tools and/or methods are conducive for future studies on this topic.

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“…The role of innate immune cells in autoimmune uveitis has been intensely investigated in the eyes of rodent EAU models ( 57 – 60 ) and infiltration of these cells in eyes of ERU horses, although to a lesser extent, has also been observed ( 3 ). The involvement of monocytes, which might promote pro-inflammatory behavior, as well as destruction of the targeted retinal cells in ERU could be shown by expression of CD68 on infiltrating peripheral immune cells in the diseased retina ( 61 ).…”

Section: Peripherymentioning

confidence: 99%

Immunological Insights in Equine Recurrent Uveitis

Degroote¹,

Deeg²

2021

Front. Immunol.

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Horses worldwide suffer from equine recurrent uveitis (ERU), an organ-specific, immune-mediated disease with painful, remitting-relapsing inflammatory attacks alternating with periods of quiescence, which ultimately leads to blindness. In course of disease, both eyes can eventually be affected and since blind horses pose a threat to themselves and their surroundings, these animals have to be killed. Therefore, this disease is highly relevant for veterinary medicine. Additionally, ERU shows strong clinical and pathological resemblance to autoimmune uveitis in man. The exact cause for the onset of ERU is unclear to date. T cells are believed to be the main effector cells in this disease, as they overcome the blood retinal barrier to invade the eye, an organ physiologically devoid of peripheral immune cells. These cells cause severe intraocular inflammation, especially in their primary target, the retina. With every inflammatory episode, retinal degeneration increases until eyesight is completely lost. In ERU, T cells show an activated phenotype, with enhanced deformability and migration ability, which is reflected in the composition of their proteome and downstream interaction pathways even in quiescent stage of disease. Besides the dysregulation of adaptive immune cells, emerging evidence suggests that cells of the innate immune system may also directly contribute to ERU pathogenesis. As investigations in both the target organ and the periphery have rapidly evolved in recent years, giving new insights on pathogenesis-associated processes on cellular and molecular level, this review summarizes latest developments in ERU research.

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Comparison of Aqueous and Vitreous Lymphocyte Populations From Two Rat Models of Experimental Uveitis

Cited by 23 publications

References 36 publications

Bioluminescence for in vivo detection of cell-type-specific inflammation in a mouse model of uveitis

Bioluminescence for in vivo detection of cell-type-specific inflammation in a mouse model of uveitis

A Review of the Various Roles and Participation Levels of B-Cells in Non-Infectious Uveitis

Immunological Insights in Equine Recurrent Uveitis

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