A complex assessment of nutrition-related health risks of the working-age population of the Samara region with application of the questionnaire method is carried out. Actual nutrition was studied by a method of 24-hour reproduction of a diet with use of the program complex «Nutri-prof» (version 2.4). We conducted anthropometric studies, evaluated component analysis of body composition, studied biochemical parameters of nutritional status. According to the results of the work it is shown that the diets of the surveyed persons are excessive in calories (due to the content of fats, added sugar), in the content of table salt, contain insufficient amounts of a number of vitamins and minerals, diets are deficient in the content of fruits, vegetables, milk and dairy products, fish, excessive in the content of animal fats, confectionery. In the group of patients examined, the dietary regimen and nature were violated, a correlation was established between the actual intake of nutrients and the level of physical development, bioimpedance measurement, biochemical parameters of nutritional status, and the relationship between nutritional status and health was established. The obtained results formed the basis for the development of a program of individual prevention of alimentary-dependent diseases, as well as one in organized labor collectives.