Hydroxyurea-induced inhibition of DNA puff development in the salivary gland chromosomes of Bradysia hygida

5-Bromo-2’-deoxyuridine induces visible morphological alteration in the DNA puffs of the anterior salivary gland region of Bradysia hygida (Diptera, Sciaridae)

“…After dissection, the S1 salivary gland regions were fixed for 2 h at room temperature in 7% perchloric acid (PCA) (13) and then squashed in a drop of 50% acetic acid using gelatinized slides and siliconized coverslips. Alternatively, this material was maintained in 7% PCA from hours to weeks at -18°C, and then it was squashed.…”

“…About 20 h later, at age E7, the first group of DNA puffs becomes transcriptionally active; 6 to 7 h after that, these puffs have already receded. At E7+12h, the second group of DNA puffs starts to expand and these puffs remain transcriptionally active until about E7+24h, near the time of the pupal molt (13,14). Additional DNA synthesis is very intense at these sites.…”

A 28-fold increase in secretory protein synthesis is associated with DNA puff activity in the salivary gland of Bradysia hygida (Diptera, Sciaridae)

“…In all experiments 1 µl of a solution of tritiated leucine ([ 3 H]-Leu) from Amersham (159 Ci/mmol) was injected into the larval hemolymph as described by Sauaia et al (22).…”

“…rated into their different regions S1, S2 and S3 by dissection (22) and at least 6 regions were dissolved in sample buffer (5 µl/region) and kept at -20 o C until the time for application to a gel.…”

Caracterização do gene CDK7 e análise de sua possível atuação nos endociclos de Rhynchosciara americana.

“…Inicialmente, sua alimentação era baseada em folhas de batata doce (Lara et al, 1965), porém esta foi modificada para erva mate (Ilex paraguariensis), conhecida como erva de chimarrão/tereré; a erva é umidificada e deixada próxima a uma estufa de secagem, o que proporciona condições de temperaturas favoráveis ao crescimento de fungos (Sauaia et al, 1971). Após alguns dias, essa erva é congelada para matar qualquer organismo que possa contaminar a cultura das larvas (nematoides e pulgões, por exemplo) e sua temperatura é restabelecida.…”