Hydroxystearic Acid Effects on CDC2/Histone H1 Kinase Activity in C108 Carcinoma Cells

Casali, Emanuela; Gesmundo, N.; Farruggia, Giovanna; Spisni, Alberto; Masotti, Lanfranco

doi:10.1006/bbrc.1994.2338

Cited by 9 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In HT29 colon cancer cells the inhibitory effect resulted in a powerful alteration of cell distribution in the cycle phases, accumulation of cells inG0/G1, and inhibition of histone H1 kinase activity 4,. 5…”

mentioning

confidence: 99%

Gas chromatography/mass spectrometric assay of endogenous cellular lipid peroxidation products: quantitative analysis of 9‐ and 10‐hydroxystearic acids

Bertucci

Hudaib

Boga

et al. 2002

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

A sensitive, specific, accurate and reproducible gas chromatography/mass spectrometry method was developed for the assay of 9- and 10-hydroxystearic acids in samples obtained as cell extracts. The preparation of the samples required specific procedures to allow the analysis of both the free and the conjugated hydroxy acids as the corresponding methyl esters. The quantification used propyl-paraben as the internal standard and monitoring of a specific fragment of each isomeric hydroxy acid methyl ester, and allowed quantification of the conjugate and the free fractions of both 9- and 10-hydroxystearic acids. This method is suitable for identification and quantification (LOQ 1.8 and 4.4 ng, respectively) of these important metabolites of lipid peroxidation. In particular the development of an assay for the free 9-hydroxystearic acid methyl ester makes the method a reliable analytical tool for investigations of the role of this metabolite in the mechanisms of tumour cell proliferation.

show abstract

mentioning

confidence: 99%

Gas chromatography/mass spectrometric assay of endogenous cellular lipid peroxidation products: quantitative analysis of 9‐ and 10‐hydroxystearic acids

Bertucci

Hudaib

Boga

et al. 2002

Rapid Comm Mass Spectrometry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Despite the relatively weak activity of aliphatic acids as HDACi, long-chain monohydroxyl fatty acids have gained increasing interest due to their biological effect on DNA synthesis and cell proliferation. Among these, 9-hydroxystearic acid (9-HSA), an endogenous lipid, whose content is strongly diminished in cancer cells, demonstrated to inhibit HDAC1 activity at micromolar concentrations, leading to the histone hyperacetylation with consequent inhibition of cancer cell growth and differentiation. − In HT29 human colorectal adenocarcinoma cells, the inhibitory effect of 9-HSA is mediated by an arrest in the G0/G1 phase cell cycle and by a direct activation of the p21 WAF1 gene …”

Section: Introductionmentioning

confidence: 99%

Unprecedented Behavior of (9R)-9-Hydroxystearic Acid-Loaded Keratin Nanoparticles on Cancer Cell Cycle

et al. 2019

View full text Add to dashboard Cite

Histone deacetylases, HDACs, have been demonstrated to play a critical role in epigenetic signaling and were found to be overexpressed in several type of cancers; therefore, they represent valuable targets for anticancer therapy. 9-Hydroxystearic acid has been shown to bind the catalytic site of HDAC1, inducing G0/G1 phase cell cycle arrest and activation of the p21WAF1 gene, thus promoting cell growth inhibition and differentiation in many cancer cells. Despite the (R) enantiomer of 9-hydroxystearic acid (9R) displaying a promising in vitro growth-inhibitory effect on the HT29 cell line, its scarce water solubility and micromolar activity require novel solutions for improving its efficacy and bioavailability. In this work, we describe the synthesis and in vitro biological profiling of 9R keratin nanoparticles (9R@ker) obtained through an in-water drug-induced aggregation process. The anticancer activity of 9R@ker was investigated in the HT29 cell line; the results indicate an increased fluidity of cell membrane and a higher intracellular ROS formation, resulting in an unexpected S phase cell cycle arrest (25% increase as compared to the control) induced by 9R@ker with respect to free 9R and an induction of cell death.

show abstract

Fluorescein conjugates of 9- and 10-hydroxystearic acids: synthetic strategies, photophysical characterization, and confocal microscopy applications

Boga¹,

Puggioli²,

Gherpelli³

et al. 2004

Analytical Biochemistry

View full text Add to dashboard Cite

Hydroxystearic Acid Effects on CDC2/Histone H1 Kinase Activity in C108 Carcinoma Cells

Cited by 9 publications

References 0 publications

Gas chromatography/mass spectrometric assay of endogenous cellular lipid peroxidation products: quantitative analysis of 9‐ and 10‐hydroxystearic acids

Gas chromatography/mass spectrometric assay of endogenous cellular lipid peroxidation products: quantitative analysis of 9‐ and 10‐hydroxystearic acids

Unprecedented Behavior of (9R)-9-Hydroxystearic Acid-Loaded Keratin Nanoparticles on Cancer Cell Cycle

Fluorescein conjugates of 9- and 10-hydroxystearic acids: synthetic strategies, photophysical characterization, and confocal microscopy applications

Contact Info

Product

Resources

About