Fluorescein conjugates of 9- and 10-hydroxystearic acids: synthetic strategies, photophysical characterization, and confocal microscopy applications

Boga, Carla; Puggioli, Silvia; Gherpelli, Marica; Farruggia, Giovanna; Pagnotta, Eleonora; Masotti, Lanfranco; Neyroz, Paolo

doi:10.1016/j.ab.2004.09.014

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…37 A decrease in potential would signal that there is a drop in the energy status of the cells possibly linked to the induction of apoptosis. 38 The expression of GFP also was documented in the experimental discs (AdBMP-7; AdBMP-10 and AdGFP); this confirms the sustained expression of a transduced gene. Specifically, on the last day of organ culture, the discs with their adjacent endplates were incubated with fresh medium containing 0.25 g/mL CellTracker Red CMTPX for 1 hour.…”

Section: Evaluation Of Implanted Cell Survival By Fluorescent Microscopymentioning

confidence: 52%

Cell Therapy Using Articular Chondrocytes Overexpressing BMP-7 or BMP-10 in a Rabbit Disc Organ Culture Model

Zhang

Phillips²,

Thonar³

et al. 2008

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Section: Evaluation Of Implanted Cell Survival By Fluorescent Microscopymentioning

confidence: 52%

Cell Therapy Using Articular Chondrocytes Overexpressing BMP-7 or BMP-10 in a Rabbit Disc Organ Culture Model

Zhang

Phillips²,

Thonar³

et al. 2008

Spine

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“…11 A decrease in membrane potential would signal that there is a drop in the energy status of the cells possibly linked to the induction of apoptosis. 29 Subsequently, the discs were incubated overnight with medium without dye to remove unbound dye. The NP tissues then were scooped out with a scalpel and mounted on glass slides.…”

Section: Methodsmentioning

confidence: 99%

Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells in the Cultured Rabbit Intervertebral Disc

Anderson

Markova

et al. 2013

American Journal of Physical Medicine &Amp; Rehabilitation

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Objective Back pain associated with symptomatic disc degeneration is a common clinical condition. Intervertebral disc (IVD) cell apoptosis and senescence increase with aging and degeneration. Repopulating the IVD with cells that could produce and maintain extracellular matrix would be an alternative therapy to surgery. The objective of this study was to determine the potential of human umbilical cord blood–derived mesenchymal stem cells (hUCB-MSCs) as a novel cell source for disc repair. In this study, we intended to confirm the potential for hUCB-MSCs to differentiate and display a chondrocyte-like phenotype after culturing in micromass and after injection into the rabbit IVD explant culture. We also wanted to confirm hUCB-MSC survival after transplantation into the IVD explant culture. Design This study consisted of micromass cultures and in vitro rabbit IVD explant cultures to assess hUCB-MSC survival and differentiation to display chondrocyte-like phenotype. First, hUCB-MSCs were cultured in micromass and stained with Alcian blue dye. Second, to confirm cell survival, hUCB-MSCs were labeled with an infrared dye and a fluorescent dye before injection into whole rabbit IVD explants (host). IVD explants were then cultured for 4 wks. Cell survival was confirmed by two independent techniques: an imaging system detecting the infrared dye at the organ level and fluorescence microscopy detecting fluorescent dye at the cellular level. Cell viability was assessed by staining the explant with CellTracker green, a membrane-permeant tracer specific for live cells. Human type II collagen gene expression (from the graft) was assessed by polymerase chain reaction. Results We have shown that hUCB-MSCs cultured in micromass are stained blue with Alcian blue dye, which suggests that proteoglycan-rich extracellular matrix is produced. In the cultured rabbit IVD explants, hUCB-MSCs survived for at least 4 wks and expressed the human type II collagen gene, suggesting that the injected hUCB-MSCs are differentiating into a chondrocyte-like lineage. Conclusions This study demonstrates the abiity of hUBC-MSCs to survive and assume a chondrocyte-like phenotype when injected into the rabbit IVD. These data support the potential for hUBC-MSCs as a cell source for disc repair. Further measures of the host response to the injection and studies in animal models are needed before trials in humans.

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“…It can be also used to monitor the localization of these biologically active molecules in living cells and provide a useful tool for linking biochemical investigation with optical visualization methods. [28][29][30][31] The combination of 5-AF and maleic anhydride with bioactive glasses has generated a large range of novel hybrid materials tailored to applications in localized drug delivery systems for bone diseases.…”

Section: Introductionmentioning

confidence: 99%

Conjugation of amino-bioactive glasses with 5-aminofluorescein as probe molecule for the development of pH sensitive stimuli-responsive biomaterials

Aina

Malavasi

Magistris

et al. 2014

J Mater Sci: Mater Med

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Bioceramics, such as silica-based glasses, are widely used in bone and teeth restoration. Nowadays, the association between nanotechnology and pharmacology is one of the most promising research fields in cancer therapy. The advanced processing methods and new chemical strategies allow the incorporation of drugs within them or on their functionalized surfaces. Bioceramics can act as local drug delivery systems to treat bone and teeth diseases. The present paper reports data related to the development of a pH-stimuli responsive bioactive glass. The glass conjugation with 5-aminofluorescein (5-AF), through a pH-sensitive organic spacer, allows to produce a pH-responsive bioactive biomaterial: when it is exposed to specific pH changes, it can favour the release of 5-AF directly at the target site. 5-AF has been chosen as a simple, low cost, non toxic model to simulate doxorubicin, an anticancer drug. As doxorubicin, 5-AF contains an amino group in its structure in order to form an amide bond with the carboxylic functionalities of the glass. Raman spectroscopy and thermal analysis confirm the glass conjugation of 5-AF by means of an amide bond; the amount of 5-AF loaded was very high (≈ 65 and 44 wt%). The release tests at two different pH (4.2 and 7.4) show that the amount of released 5-AF is higher at acid pH with respect to physiological one. This preliminary datum evidenced that a pH-sensitive drug delivery system has been developed. The low amount of 5-AF released (<1 wt% of the total 5-AF) is due to the very low solubility of 5-AF in aqueous medium. This disadvantage, may be overcome in a dynamic environment (physiological conditions), where it is possible to obtain a drug release system ensuring an effective therapeutic dose for long times and, at the same time, avoiding the drug toxicity.

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Fluorescein conjugates of 9- and 10-hydroxystearic acids: synthetic strategies, photophysical characterization, and confocal microscopy applications

Cited by 5 publications

References 15 publications

Cell Therapy Using Articular Chondrocytes Overexpressing BMP-7 or BMP-10 in a Rabbit Disc Organ Culture Model

Cell Therapy Using Articular Chondrocytes Overexpressing BMP-7 or BMP-10 in a Rabbit Disc Organ Culture Model

Human Umbilical Cord Blood–Derived Mesenchymal Stem Cells in the Cultured Rabbit Intervertebral Disc

Conjugation of amino-bioactive glasses with 5-aminofluorescein as probe molecule for the development of pH sensitive stimuli-responsive biomaterials

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