Hydroxysafflor yellow A suppresses inflammatory responses of BV2 microglia after oxygen–glucose deprivation

Li, Jie; Zhang, Shenyang; Lu, Mengru; Chen, Zhibin; Chen, Cong; Han, Lijuan; Zhang, Meijuan; Xu, Yun

doi:10.1016/j.neulet.2012.12.056

Cited by 61 publications

(38 citation statements)

References 20 publications

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“…To this end, three different paradigms were applied to activated microglia in vitro; i.e., LPS stimulation, direct OGD stimulation and stimulation with OGD-conditioned neuronal buffer. Specifically, the latter two in vitro models have been used to mimic the in vivo activation of microglia following cerebral ischemia (Hu et al, 2012;Li et al, 2013). In accordance with the in vivo results, let-7c-5p levels were remarkably decreased in the activated microglia, and let-7c-5p overexpression blunted the activation-induced production of proinflammatory mediators.…”

Section: Discussionsupporting

confidence: 53%

MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation

Wang

Chen

et al. 2015

Brain, Behavior, and Immunity

148

104

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Section: Discussionsupporting

confidence: 53%

MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation

Wang

Chen

et al. 2015

Brain, Behavior, and Immunity

148

104

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“…[14][15][16][17] It has been reported that HSYA inhibits the phosphorylation of NF-κB and p38 MAPK in BV2 cells in OGD/reoxygeneration (OGD/R) conditions, accompanied by the downregulation of inflammatory IL-1β, IL-6, and TNF-α. 18 Human epithelial cell strain A549 has been stimulated by LPS to activate the TLR4/ Myd88/NF-κB inflammatory pathway, while HYSA also reduces TLR4 expression on mRNA and protein levels. 19 In many CNS disorders, microglia are the cells principally responsible for the development of a neuroinflammatory microenvironment.…”

Section: Safflower Yellow Regulates Microglial Polarization and Inhibmentioning

confidence: 99%

Safflower Yellow regulates microglial polarization and inhibits inflammatory response in LPS-stimulated Bv2 cells

Yang

Zhang

et al. 2015

Int J Immunopathol Pharmacol

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Activated microglia, especially polarized M1 cells, produce pro-inflammatory cytokines and free radicals, thereby contributing directly to neuroinflammation and various brain disorders. Given that excessive or chronic neuroinflammation within the central nervous system (CNS) exacerbates neuronal damage, molecules that modulate neuroinflammation are candidates as neuroprotective agents. In this study, we provide evidence that Safflor yellow (SY), the main active component in the traditional Chinese medicine safflower, modulates inflammatory responses by acting directly on BV2 microglia. LPS stimulated BV2 cells to upregulate expression of TLR4-Myd88 and MAPK-NF-κB signaling pathways and to release IL-1β, IL-6, TNF-α, and COX-2. However, SY treatment inhibited expression of TLR4-Myd88 and p-38/p-JNK-NF-κB, downregulated expression of iNOS, CD16/32, and IL-12, and upregulated CD206 and IL-10. In conclusion, our results demonstrate that SY exerts an anti-inflammatory effect on BV2 microglia, possibly through TLR-4/p-38/p-JNK/ NF-κB signaling pathways and the conversion of microglia from inflammatory M1 to an anti-inflammatory M2 phenotype.

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“…According to the biology process of up and down-regulated coding genes, leukocytes stored at RT possibly underwent apoptosis and neutrophils activation. Blood cells preserved at EDTA tubes are under glucose and hypoxia deprivation that promotes cascades of biological alterations, causing inflammation [34, 35] and triggering neutrophils apoptosis [36-38]. As RT has a major impact on leukocyte transcriptome, the influence of temperature should not be ignored when dealing with samples stored at such a condition.…”

Section: Discussionmentioning

confidence: 99%

Effect of storage conditions on peripheral leukocytes transcriptome

Xing

Yang

Chen

et al. 2020

Preprint

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Introduction: Peripheral blood leukocytes are essential components of the innate and adaptive immune responses. Their transcriptome reflects an individual's physiological and pathological state, consequently bulk and single-cell RNA sequencing have been used to assess health and disease. As RNA is dynamic and could be affected by ex vivo conditions before RNA stabilization, we have assessed the influence of temporary storage on the transcriptome. Methods:We collected peripheral blood from six healthy donors and processed it immediately or stored it either at 4 or room temperature (RT,(18)(19)(20)(21)(22) for 2h, 6h and 24h. Total cellular RNA was extracted from leukocytes after red blood cells lysis and the transcriptome analyzed using RNA sequencing. Results:We identified 152 up-regulated and 12 down-regulated coding genes in samples stored at 4 for 24h with most of the up-regulated genes related to nucleosome assembly. More coding genes changed expression at RT, with 1218 increased and 1480 decreased. The increased genes were particularly related to mRNA processing and apoptosis, while the decreased genes were associated with neutrophil activation and cytokine production, implying a reduced proportion of neutrophils, which was confirmed by leukocyte subsets analysis. Most house-keeping genes changed expression during storage, but genes such as TUBB and C1orf43 3 / 2 1 were relatively stable and could serve as reference genes. Conclusion: Temporary storage conditions profoundly affect leukocyte gene expression profiles and change leukocyte subset proportions. Blood samples stored at 4 for 6h largely maintain their original transcriptome. / 2 1 Materials and Methods Sample collectionPeripheral blood samples were obtained from three healthy women and three healthy men and were processed immediately or stored either at RT or 4 for 2h, 6h and 24h ( Fig. 1A). Leukocytes were subsequently isolated for RNA-seq. RNA isolation and sequencingRNA from leukocytes was extracted by the TRIzol Reagent (Thermo Fisher, 10296028) following standard procedures as previously described [13]. RNA concentration and integrity were measured and calculated by Agilent 2100 bioanalyzer (Agilent Technologies, G2939A) and accompanying software. All RNA samples were with amounts greater than 200ng and RIN values of greater than 6 underwent library construction. Libraries were built using RNase H method [14] and sequenced on BGISEQ-500RS (single-end 50bp). Data preprocessingRibosomal ribonucleic acid (rRNA) was removed by SOAP2 (Version 2.21t) [15] and reads with low quality and adaptors was filtered by SOAPnuke (Version 1.5.6) [16] from raw data based on default parameters. Clean data were obtained in the FASTQ format. Kallisto [17] was used to align all clean reads to transcriptome references and obtain estimated reads counts and transcripts per million mapped reads (TPM) 7 / 2 1 results. Transcriptome references were the refMrna.fa.gz file from the UCSC database with the removal of NR_RNA [18] and the NONCODEv5_human.fa.gz file from the...

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Hydroxysafflor yellow A suppresses inflammatory responses of BV2 microglia after oxygen–glucose deprivation

Cited by 61 publications

References 20 publications

MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation

MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation

Safflower Yellow regulates microglial polarization and inhibits inflammatory response in LPS-stimulated Bv2 cells

Effect of storage conditions on peripheral leukocytes transcriptome

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