MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation

Ni, Jingen; Wang, Xiaoyu; Chen, Shuangshuang; Liu, Huihui; Wang, Yun; Xu, Xingshun; Cheng, Jian; Jia, Jia; Zhen, Xuechu

doi:10.1016/j.bbi.2015.04.014

Cited by 141 publications

(102 citation statements)

References 31 publications

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“…Decreased levels of let-7c-5p was observed in the plasma of stroke patients as well as in the plasma and brain of mice subjected to transient focal ischemia (Ni et al, 2015). When let-7c-5p was overexpressed, levels of caspase-3 were decreased leading to reduced microglial activation, decreased infarct volume and neurological deficits after focal ischemia (Ni et al, 2015) (Fig. 1).…”

Section: Micrornas and Post-stroke Pathophysiologymentioning

confidence: 99%

Non-coding RNAs and neuroprotection after acute CNS injuries

Chandran

Mehta

2017

Neurochemistry International

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Accumulating evidence indicates that various classes of non-coding RNAs (ncRNAs) including microRNAs (miRNAs), PIWI-interacting RNAs (piRNAs) and long non-coding RNAs (lncRNAs) play important roles in normal state as well as the diseases of the CNS. Interestingly, ncRNAs have been shown to interact with messenger RNA, DNA and proteins, and these interactions could induce epigenetic modifications and control transcription and translation, thereby adding a new layer of genomic regulation. The ncRNA expression profiles are known to be altered after acute CNS injuries including stroke, traumatic brain injury and spinal cord injury that are major contributors of morbidity and mortality worldwide. Hence, a better understanding of the functional significance of ncRNAs following CNS injuries could help in developing potential therapeutic strategies to minimize the neuronal damage in those conditions. The potential of ncRNAs in blood and CSF as biomarkers for diagnosis and/or prognosis of acute CNS injuries has also gained importance in the recent years. This review highlighted the current progress in the understanding of the role of ncRNAs in initiation and progression of secondary neuronal damage and their application as biomarkers after acute CNS injuries.

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Section: Micrornas and Post-stroke Pathophysiologymentioning

confidence: 99%

Non-coding RNAs and neuroprotection after acute CNS injuries

Chandran

Mehta

2017

Neurochemistry International

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“…Long et al found that the circulating let-7b was lower in IS patients with large vessel atherosclerosis but had higher level in patients with other kinds of IS until 24 weeks [29]. Ni et al reported that let-7c-5p in serum was significantly decreased in ischemic stroke patients as well as in experimental animals [32]. However, let-7e in serum was upregulated in young stroke patients, and the expression is relatively high in IS patients with high NIHSS score [16].…”

Section: Discussionmentioning

confidence: 96%

“…In our early animal and cellular studies, we found that let-7e was associated with cellular apoptosis in ischemic brain and hypoxia PC12 cells by regulation of caspase 3 expression. Similarly, let-7c-5p was suggested likely contributing to stroke pathogenesis by regulation of caspase 3 activation [32]. Moreover, activation of Toll-like receptor 7 by extracellular let-7 may induce neuronal cell death [34].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA let-7e Is a Potential Circulating Biomarker of Acute Stage Ischemic Stroke

Peng

Yuan

et al. 2015

Transl. Stroke Res.

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The aim of this study is to determine the expression levels and clinical significance of circulating microRNAs (miRNAs), let-7e and miR-338 at different stages following ischemic stroke (IS). Seventy-two patients with IS at the acute stage were enrolled and monitored at different stages, and 51 healthy volunteers were served as the normal controls. Expression of let-7e and miR-338 in serum and cerebral spinal fluid (CSF) samples was analyzed by real-time quantitative PCR. The relationship between expression levels of let-7e and miR-338, National Institutes of Health Stroke Scale (NIHSS) scores, and the levels of serum CRP was analyzed, respectively. Compared to healthy controls, serum let-7e expression levels were significantly increased, while serum miR-338 expression levels were slightly increased in IS patients. Expression levels of Let-7e in serum varied at different stages in IS patients with the lowest expression in the recover stage and highest expression in the acute stage. However, serum miR-338 expression in IS patients was not significantly different in any stage. Compared to healthy controls and nonacute stages of IS groups, let-7e expression in CSF was markedly upregulated in IS patients at the acute stage. Different from that of let-7e, miR-338 expression in CSF was upregulated in IS patients only at the subacute stage but not in the acute stage. Meanwhile, let-7e, which was not significantly correlated with NIHSS scores (r = 0.29, P > 0.05), was positively correlated with the serum CRP levels (r = 0.67, P = 0.033). There is no significant correlation between the miR-338 expression levels and NIHSS scores or serum CRP levels. Moreover, let-7e, but not miR-338, had a high consistency in expression when tested both in CSF and serum samples. Finally, serum let-7e showed a specificity up to 73.4 % and a sensitivity of 82.8 % in IS patients at the acute stage, whereas serum miR-338 in IS patients showed a specificity up to 53.2 % and a sensitivity of 71.9 % in the acute stage. Expression levels of let-7e in serum may serve as a useful noninvasive circulating biomarker for the acute stage of ischemic stroke.

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“…Neural stem/progenitor cells Promote proliferation miR-25 [12]; miR-137 [13]; miR-184 [14]; miR-195 [15] Induce differentiation miR-9, siRNA-TLX [16]; let-7d [17]; miR-137 [13]; miR-184 [14]; miR-195 [15]; miR-34a [18]; lncRNA-BDNF-AS, siRNA-BDNF-AS [19] Mesenchymal stem cells Induce differentiation miR-9 [20]; miR-124 [21] Reduce differentiation miR-128 [22] Neuronal cells Inhibit cell death miR-223 [23]; miR-181c [24]; miR-592 [25]; miR-424 [26]; miR-23a-3p [27]; miR-23a/b, miR-27a/b, siRNA-Apaf-1 [28] Promote cell death miR-134 [29]; miR-200c [30]; miR-30a/b [31][32][33]; miR-124 [34]; miR-711 [35] Regulate degeneration and apoptosis miR-20a [36]; miR-29b [37]; miR-146a, siRNA-miR146a [38] Promote neurite outgrowth miR-7 [39]; miR-21 [40]; miR-222, siRNA-PTEN [41]; miR-8 [42]; miR-431 [43]; miR-145 [44]; lncRNA-uc.217 [45]; miR-138, siRNA-SIRT1 [46] Microglial cells Inhibit inflammation let-7c [47]; miR-124, siRNA-C/EBP-α [48] Promote pro-inflammation miR-155 [49] Inhibit activation let-7c-5p [50] Astrocytes P...…”

Section: Nervementioning

confidence: 99%

Noncoding RNAs and Their Potential Therapeutic Applications in Tissue Engineering

Qian

Wang

et al. 2017

Engineering

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Tissue engineering is a relatively new but rapidly developing field in the medical sciences. Noncoding RNAs (ncRNAs) are functional RNA molecules without a protein-coding function; they can regulate cellular behavior and change the biological milieu of the tissue. The application of ncRNAs in tissue engineering is starting to attract increasing attention as a means of resolving a large number of unmet healthcare needs, although ncRNA-based approaches have not yet entered clinical practice. In-depth research on the regulation and delivery of ncRNAs may improve their application in tissue engineering. The aim of this review is: to outline essential ncRNAs that are related to tissue engineering for the repair and regeneration of nerve, skin, liver, vascular system, and muscle tissue; to discuss their regulation and delivery; and to anticipate their potential therapeutic applications.

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MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation

Cited by 141 publications

References 31 publications

Non-coding RNAs and neuroprotection after acute CNS injuries

Non-coding RNAs and neuroprotection after acute CNS injuries

MicroRNA let-7e Is a Potential Circulating Biomarker of Acute Stage Ischemic Stroke

Noncoding RNAs and Their Potential Therapeutic Applications in Tissue Engineering

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